Amplification of self-ligated, circularized cDNA for expression profiling
    32.
    发明授权
    Amplification of self-ligated, circularized cDNA for expression profiling 有权
    用于表达谱的自连接的环化cDNA的扩增

    公开(公告)号:US07309571B2

    公开(公告)日:2007-12-18

    申请号:US10770657

    申请日:2004-02-03

    申请人: John Nelson

    发明人: John Nelson

    IPC分类号: C12Q1/68 C12P19/34

    摘要: This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.

    摘要翻译: 本发明提出了一种新的cDNA扩增方法。 RNA首先转化为cDNA。 cDNA的合成可以包括启动子标记的寡核苷酸,然后将该cDNA连接形成环(或可能是连接体)。 然后使用基于Phi29 DNA聚合酶的滚动圆和链置换扩增来扩增。 本发明允许将RNA启动子序列连接到cDNA,以便通过从扩增的cDNA产生RNA来进一步扩增。 所得产物可用于制备用于基因表达研究或其他RNA分析程序的材料。

    INSECT CONTROL COMPOSITIONS
    33.
    发明申请
    INSECT CONTROL COMPOSITIONS 审中-公开
    防护组合物

    公开(公告)号:US20070264294A1

    公开(公告)日:2007-11-15

    申请号:US11382574

    申请日:2006-05-10

    IPC分类号: A01N25/00

    摘要: Disclosed herein are sprayable insect repellent compositions that provide improved skin feel through the incorporation of a powder ingredient. The powder is essentially permanently suspended in the composition by a thickener (e.g. carbomer thickener) so that no shaking to resuspend settled out powder is necessary immediately before the composition is applied to the skin. This is achieved in an aerosol or spritz spray formulation, while avoiding clogging problems. One composition contains ethanol, DEET or picaridin, carbomer thickener, a neutralizer, corn starch powder, water, and gas propellant.

    摘要翻译: 本文公开了可喷雾的驱虫剂组合物,其通过掺入粉末成分来提供改善的皮肤感觉。 该粉末通过增稠剂(例如卡波姆增稠剂)基本上永久地悬浮在组合物中,使得在将组合物施用于皮肤之前不需要立即振荡以重悬沉淀出的粉末。 这可以在气雾剂或喷雾剂喷雾配方中实现,同时避免堵塞问题。 一种组合物含有乙醇,DEET或氨基吡啶,卡波姆增稠剂,中和剂,玉米淀粉粉末,水和气体推进剂。

    Stone fabrication positioning system
    34.
    发明申请
    Stone fabrication positioning system 审中-公开
    石材制造定位系统

    公开(公告)号:US20070161331A1

    公开(公告)日:2007-07-12

    申请号:US11595378

    申请日:2006-11-10

    申请人: John Nelson

    发明人: John Nelson

    IPC分类号: B24B51/00 B24B7/30

    CPC分类号: B24B41/068 B24B9/06 B28D7/04

    摘要: A stone cutting system effective for mechanically delineating defined datum points in a Cartesian coordinate system by positioning at least one datum point defining means per coordinate comprising a combination blocking and clamping device, a table of a computer numerically controlled (CNC) stoneworking machine, a locating means positioned on the table, the blocking and clamping device being in the form of a parallelepiped including a bottom surface having a plurality of apertures adapted to enable positioning about the locating means, a top surface having a first blocking edge and a second clamping edge, including at least one means for clamping a workpiece within said top surface second clamping edge, a left side surface, a right side surface, a front surface, a rear surface, and at least one means for fixation of said device on said table.

    摘要翻译: 一种石材切割系统,其有效地通过定位至少一个基准点来定义笛卡尔坐标系中的定义的基准点,每个坐标包括组合阻塞和夹紧装置,计算机数字控制(CNC)制作机的工作台,定位 位于桌子上的装置,阻塞和夹紧装置为平行六面体形式,包括底表面,底面具有多个适于能够围绕定位装置定位的孔口,顶表面具有第一阻挡边缘和第二夹紧边缘, 包括用于将工件夹持在所述顶表面第二夹紧边缘,左侧表面,右侧表面,前表面,后表面中的至少一个装置以及用于将所述装置固定在所述工作台上的至少一个装置。

    Microreplicated article with moire reducing surface
    36.
    发明申请
    Microreplicated article with moire reducing surface 审中-公开
    具有莫尔条纹表面的微复制品

    公开(公告)号:US20060209428A1

    公开(公告)日:2006-09-21

    申请号:US11369482

    申请日:2006-03-06

    IPC分类号: G02B3/08

    摘要: A microreplicated article having a moiré reducing surface and method of manufacturing the same, are disclosed. A microreplicated article includes a flexible substrate having first and second opposed surfaces, a first coated microreplicated pattern on the first surface, and a second coated microreplicated pattern on the second surface. The first coated microreplicated pattern and the second coated microreplicated pattern are registered to within 10 micrometers in a machine direction and a transverse direction and the first coated microreplicated pattern and second coated microreplicated pattern form a plurality of lens segments. Each lens segment includes a plurality of lens elements each having an optical axis where all of the lens element optical axes are parallel to each other and lens elements within a first lens segment have optical axes that are offset from optical axes of lens elements within an adjacent second lens segment.

    摘要翻译: 公开了具有莫尔面还原表面的微复制品及其制造方法。 微复制品包括具有第一和第二相对表面的柔性基底,第一表面上的第一涂覆的微复制图案和在第二表面上的第二涂覆的微复制图案。 第一涂布的微复制图案和第二涂布的微复制图案在机器方向和横向方向上记录在10微米内,并且第一涂布的微复制图案和第二涂布的微复制图案形成多个透镜段。 每个透镜段包括多个透镜元件,每个透镜元件具有其中所有透镜元件光轴彼此平行的光轴,并且第一透镜段内的透镜元件具有偏离相邻的透镜元件的透镜元件的光轴的光轴 第二透镜段。

    Methods and systems for testing materials
    38.
    发明申请
    Methods and systems for testing materials 审中-公开
    测试材料的方法和系统

    公开(公告)号:US20060153739A1

    公开(公告)日:2006-07-13

    申请号:US11034566

    申请日:2005-01-13

    IPC分类号: G01N21/62

    CPC分类号: G01N22/00

    摘要: Methods and systems for evaluating a material sample is provided. The system includes a material sample holder including a first flange having an aperture therethrough, a second flange having an aperture therethrough, the first and second flanges configured to frictionally hold a material sample sandwiched therebetween, a waveguide coupled to a first end of each of the first flange and the second flange, each waveguide configured to direct electromagnetic waves through respective apertures, a waveguide adapter communicatively coupled to a second end of each waveguide, and a control unit electrically coupled to the wave source, the control unit configured to control the waveguide adapter to transmit and receive electromagnetic wave signals.

    摘要翻译: 提供了用于评估材料样品的方法和系统。 该系统包括材料样品保持器,其包括具有穿过其中的孔的第一凸缘,具有穿过其中的孔的第二凸缘,所述第一和第二凸缘被配置为摩擦地夹持夹在其间的材料样品,耦合到每个的第一端 第一凸缘和第二凸缘,每个波导被配置成将电磁波引导通过相应的孔,通信地耦合到每个波导的第二端的波导适配器和电耦合到波源的控制单元,该控制单元被配置为控制波导 适配器发送和接收电磁波信号。

    Landfill cover composition and method
    39.
    发明申请
    Landfill cover composition and method 审中-公开
    垃圾填埋覆盖物的组成和方法

    公开(公告)号:US20050220542A1

    公开(公告)日:2005-10-06

    申请号:US10814439

    申请日:2004-03-31

    摘要: A landfill cover composition comprising a concentrate formulated to include a hardening agent, a thickener and emulsifier, and a super absorbent polymer, mixable with water and a filler material, such as cellulose or wood fiber mulch. A mixture of water, filler material and concentrate is sprayed on the surface of a landfill refuse layer and hardened to provide at least a temporary cover as an alternative to a significantly thicker or deeper layer of conventional soil or earth cover. The biodegradable composition preferably includes Plaster of Paris and guar gum compositions and cross-linked sodium acrylate polymer fines, mixed with the filler material and water at a landfill site for reducing transportation and handling costs.

    摘要翻译: 一种填埋覆盖物组合物,其包含配制成包含硬化剂,增稠剂和乳化剂以及与水和填料如纤维素或木纤维覆盖物混合的高吸水性聚合物的浓缩物。 将水,填充材料和浓缩物的混合物喷洒在垃圾填埋物垃圾层的表面上并硬化以提供至少一个临时盖,作为常规土壤或土壤覆盖物的较厚或较深层的替代物。 可生物降解的组合物优选包括巴黎石膏和瓜尔胶组合物和交联的丙烯酸钠聚合物细粉,与填埋材料和水在填埋场混合以降低运输和处理成本。

    Capsule filling device and method of operation
    40.
    发明授权
    Capsule filling device and method of operation 失效
    胶囊灌装装置及其操作方法

    公开(公告)号:US06901972B1

    公开(公告)日:2005-06-07

    申请号:US10726444

    申请日:2003-12-03

    申请人: John Nelson

    发明人: John Nelson

    IPC分类号: A61J3/07 B65B1/04 B67C3/02

    CPC分类号: A61J3/074

    摘要: A device and method of operation for filling medicine capsules, in which a fixed quantity of open capsules to be filled are supported in a circular array by a holder plate beneath the periphery of a rotatable distribution drum. In use, a measured supply of filling medication, in dry granular form, is dispensed into the center of the rotating drum where it is distributed evenly by centrifugal force along the periphery of the drum. As the drum stops rotating, the medication flows by gravity into the capsules, which are then inspected, closed and packaged. The distribution of filler material is more even than if the capsules were filled by hand, and the average quantity (both by volume and by weight) of the filling material per capsule in the batch is variable to suit an individual patient's needs, and very accurately known. In addition, two or more compatible filler medications may be mixed in the same capsules to suit the specialized needs of a particular user. Because the provided quantity of medication is completely distributed into each batch of capsules, inadvertent double-filling is prevented.

    摘要翻译: 一种用于填充药胶囊的装置和操作方法,其中固定量的待填充的开放胶囊通过可旋转分配鼓的外围下方的保持板以圆形阵列支撑。 在使用中,以干燥颗粒形式的测量的填充药物供应被分配到旋转鼓的中心,其中通过离心力沿鼓的周边均匀分布。 当鼓停止旋转时,药物通过重力流入胶囊中,然后检查,关闭和包装。 填充材料的分布比手动填充胶囊更均匀,并且批次中每个胶囊的填充材料的平均数量(以体积计和重量)是可变的,以适应个别患者的需要,并且非常精确 已知 此外,两种或多种相容的填充药物可以混合在相同的胶囊中以适应特定用户的特殊需要。 由于所提供的药物量完全分配到每批胶囊中,因此无意中进行双重填充。