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31.发明申请公开(公告)号：US20220074858A1
公开(公告)日：2022-03-10
申请号：US17400741
申请日：2021-08-12
Applicant: Becton, Dickinson and Company
Inventor： Bing Shan , Jizuo Zou , Svitlana Berezhna , Eric D. Diebold , An-Dien Nguyen , Fedor Ilkov , Mark Dorighi
Abstract: Aspects of the disclosure include systems for irradiating a sample in a flow stream. Systems according to certain embodiments include a light propagator having a first fiber optic operably coupled to a first laser and configured to receive light from the first laser at a proximal end and to convey the laser light from a distal end to a first position on the flow stream and a second fiber optic operably coupled to a second laser and configured to receive light from the second laser at a proximal end and to convey the laser light from a distal end to a second position on the flow stream. Methods for irradiating a sample in a flow stream with the subject systems and detecting light from two or more positions on irradiated flow stream are also provided.
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公开(公告)号：US20200209140A1
公开(公告)日：2020-07-02
申请号：US16814394
申请日：2020-03-10
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：USD872154S1
公开(公告)日：2020-01-07
申请号：US29638541
申请日：2018-02-27
Applicant: Becton, Dickinson and Company
Designer： Eric D. Diebold , Jorge Manzarraga
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34.发明申请公开(公告)号：US20190383725A1
公开(公告)日：2019-12-19
申请号：US16422630
申请日：2019-05-24
Applicant: Becton, Dickinson and Company
Inventor： Timothy Petersen , Eric D. Diebold , Bob Cao , Bernard Johanna Verwer
IPC: G01N15/14
Abstract: Systems for differentially detecting light (e.g., in a flow stream) are described. Light detection systems according to certain embodiments include a plurality of photodetectors, an amplifier component and an electronic switch component having a plurality of switches in electrical communication with the plurality of photodetectors and the amplifier component. Systems and methods for differentially detecting light and optimizing the measurement of light emitted by a sample (e.g., in a flow stream) are also described. Kits having a photodetector array, an amplifier component and an electronic switch component are also provided.
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公开(公告)号：US20180275042A1
公开(公告)日：2018-09-27
申请号：US15992097
申请日：2018-05-29
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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Patent Agency Ranking
公开(公告)号：US20240110859A1
公开(公告)日：2024-04-04
申请号：US18529323
申请日：2023-12-05
Applicant: Becton, Dickinson and Company
Inventor： Timothy Wayne Petersen , Pierce O. Norton , Henry J. Lankila , Eric D. Diebold
CPC classification number: G01N15/1434 , G01R27/02 , H03F3/08 , G01N2015/149
Abstract: Aspects of the present disclosure include methods for adjusting sensitivity of a photodiode in a light detection system. Methods according to certain embodiments include detecting light with a light detection system having a photodiode and an amplifier, determining responsivity of the photodiode over a plurality of wavelengths of light and adjusting one or more parameters of the amplifier in response to the responsivity of the photodiode over the plurality of wavelengths of light. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodiode and an amplifier for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.
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公开(公告)号：US11940369B2
公开(公告)日：2024-03-26
申请号：US18214317
申请日：2023-06-26
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/14 , G01N15/1425 , G01N15/1429 , G01N15/147 , G01N15/1475 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
Abstract: Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.
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公开(公告)号：US11692926B2
公开(公告)日：2023-07-04
申请号：US17745249
申请日：2022-05-16
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/14 , G01N15/147 , G01N15/1425 , G01N15/1429 , G01N15/1475 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US20220268687A1
公开(公告)日：2022-08-25
申请号：US17745249
申请日：2022-05-16
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US11366052B2
公开(公告)日：2022-06-21
申请号：US17153527
申请日：2021-01-20
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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