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    Feeder layer and serum independent embryonic stem cells
    34.
    发明授权
    Feeder layer and serum independent embryonic stem cells 失效
    饲养层和血清独立胚胎干细胞

    公开(公告)号：US07641897B2

    公开(公告)日：2010-01-05

    申请号：US11286088

    申请日：2005-11-23

    申请人： Irving L. Weissman ,  Toshiyuki Yamane ,  Scott Dylla

    发明人： Irving L. Weissman ,  Toshiyuki Yamane ,  Scott Dylla

    IPC分类号： A61K48/00 ,  C12N5/16 ,  C12N15/00

    CPC分类号： C12N5/0606 ,  C12N2500/90 ,  C12N2501/235 ,  C12N2501/48 ,  C12N2510/00

    摘要： Undifferentiated primordial stem cells are manipulated to permit their long term growth in defined media lacking serum and feeder layer cells by shifting the apoptotic balance of the cells, through increasing the activity of Bcl-2 family anti-apoptotic proteins or decreasing the activity of Bcl-2 family pro-apoptotic proteins. In some embodiments of the invention, the Bcl family protein is Bcl-2. The ES cells sustain the characteristics of undifferentiated, pluripotent stem cells during long-term serum- and feeder layer cell-free conditions, including the ability to be expanded in vitro, but maintain their potential to differentiate into mature cell types.

    摘要翻译： 通过改变细胞的凋亡平衡，通过增加Bcl-2家族抗凋亡蛋白的活性或降低Bcl-2家族的抗凋亡蛋白的活性，操作未分化的原始干细胞以允许其在缺乏血清和饲养层细胞的限定培养基中长期生长， 2家庭促细胞凋亡蛋白。 在本发明的一些实施方案中，Bcl家族蛋白是Bcl-2。 ES细胞在长期血清和饲养层无细胞条件下维持未分化，多能干细胞的特征，包括体外扩增能力，但保持其分化成成熟细胞类型的潜力。

    Mammalian common lymphoid progenitor cell
    35.
    发明授权
    Mammalian common lymphoid progenitor cell 有权
    哺乳动物常见淋巴祖细胞

    公开(公告)号：US07297329B2

    公开(公告)日：2007-11-20

    申请号：US11093951

    申请日：2005-03-29

    申请人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    发明人： Koichi Akashi ,  Irving L. Weissman ,  Motonari Kondo

    IPC分类号： A01N65/00

    CPC分类号： C12N5/0635 ,  A61K38/00 ,  C12N5/0647 ,  C12N2501/125 ,  C12N2501/23 ,  C12N2501/26 ,  C12N2510/00

    摘要： A substantially enriched mammalian hematopoietic cell subpopulation is provided, which is characterized by progenitor cell activity for lymphoid lineages, but lacking the potential to differentiate into myeloid and erythroid lineages. Methods are provided for the isolation and culture of this common lymphoid progenitor cell (CLP). The cell enrichment methods employ reagents that specifically recognize CDw127 (IL-7 receptor α); CD117 (c-kit) protein, in conjunction with other markers expressed on lineage committed cells. The murine cells are also characterized as expressing low levels of sca-1 (Ly-6E and Ly-6A). The CLPs are predominantly cycling, blast cells. These cells give rise to B cells, T cells and natural killer cells, as evidenced by their growth and differentiation in vitro and in vivo.

    摘要翻译： 提供了基本上富集的哺乳动物造血细胞亚群，其特征在于淋巴谱系的祖细胞活性，但缺乏分化为骨髓和红系谱系的潜力。 提供了用于分离和培养这种常见淋巴祖细胞（CLP）的方法。 细胞富集方法使用特异性识别CDw127（IL-7受体α）的试剂; CD117（c-kit）蛋白，与在谱系确定细胞上表达的其他标志物结合。 鼠细胞也被表征为表达低水平的sca-1（Ly-6E和Ly-6A）。 CLP主要是循环，胚细胞。 这些细胞产生B细胞，T细胞和自然杀伤细胞，这通过其体外和体内的生长和分化证明。

    Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors
    36.
    发明申请
    Comprehensive Methylome Map of Myeloid and Lymphoid Commitment from Hematopoietic Proenitors 审中-公开
    来自造血干细胞的骨髓和淋巴细胞承诺的综合甲基化图

    公开(公告)号：US20130281304A1

    公开(公告)日：2013-10-24

    申请号：US13814460

    申请日：2011-08-12

    申请人： Andrew P. Feinberg ,  Irving L. Weissman

    发明人： Andrew P. Feinberg ,  Irving L. Weissman

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6881 ,  C12Q2600/154

    摘要： Provided herein are differentially methylated regions (DMRs) of multipotent progenitor cells (MPPs) and oligopotent progenitor cells and methods of use thereof. The invention provides methods for detecting and analyzing alterations in the methylation status of DMRs in such progenitor cells as well as methods for differentiating such cells.

    摘要翻译： 本文提供了多能祖细胞（MPP）和寡能祖细胞的差异甲基化区域（DMR）及其使用方法。 本发明提供了用于检测和分析这些祖细胞中DMR的甲基化状态的变化的方法以及用于分化这些细胞的方法。

    Methods and Compositions for Treating Carcinoma Stem Cells
    38.
    发明申请
    Methods and Compositions for Treating Carcinoma Stem Cells 审中-公开
    治疗癌细胞干细胞的方法和组合

    公开(公告)号：US20110124032A1

    公开(公告)日：2011-05-26

    申请号：US12865092

    申请日：2009-01-30

    申请人： Maximilian Diehn ,  Michael Clarke ,  Irving L. Weissman

    发明人： Maximilian Diehn ,  Michael Clarke ,  Irving L. Weissman

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/57496 ,  G01N33/5011 ,  G01N33/57407 ,  G01N33/57415 ,  G01N33/57419 ,  G01N2800/52

    摘要： Cancer stem cells (CSCs) have been prospectively isolated or identified from primary tumor samples, and shown to possess the unique properties of self-renewal and differentiation, and can form unique histological microdomains useful in cancer diagnosis. Such cancer stem cells are shown herein to have the phenotype of containing decreased levels of reactive oxygen species (ROS) relative to non-tumorigenic (non-stem cell) cancer cells, as well as expression of other protective pathways. The CSCs are further shown to be more resistant to ionizing radiation (IR) and certain chemotherapies and to express high levels of ROS genes.

    摘要翻译： 已经从原发性肿瘤样品前瞻性分离或鉴定了癌干细胞（CSCs），并显示其具有自我更新和分化的独特性质，并且可以形成用于癌症诊断的独特的组织学微区域。 这种癌干细胞在本文中显示为具有相对于非致瘤性（非干细胞）癌细胞降低的活性氧（ROS）水平以及其它保护途径的表达的表型。 进一步证明CSCs对电离辐射（IR）和某些化学疗法更具抗性，并表达高水平的ROS基因。

    Identification and Isolation of Acute Myeloid Leukemia Stem Cells
    39.
    发明申请
    Identification and Isolation of Acute Myeloid Leukemia Stem Cells 有权
    鉴定和分离急性骨髓性白血病干细胞

    公开(公告)号：US20100255575A1

    公开(公告)日：2010-10-07

    申请号：US12817105

    申请日：2010-06-16

    申请人： Irving L. Weissman ,  Naoki Hosen

    发明人： Irving L. Weissman ,  Naoki Hosen

    IPC分类号： C12N5/09

    CPC分类号： G01N33/57426 ,  C12Q1/34 ,  G01N33/5011

    摘要： Acute myeloid leukemia stem cells (AMLSC) are identified. The cells can be prospectively isolated or identified from patient samples, and are shown to possess the unique properties of cancer stem cells in functional assays for cancer stem cell self-renewal and differentiation, and in cancer diagnosis.

    摘要翻译： 确定急性骨髓性白血病干细胞（AMLSC）。 可以从患者样品前瞻性分离或鉴定细胞，并且在癌症干细胞自我更新和分化以及癌症诊断的功能测定中显示具有癌干细胞的独特性质。