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65 条记录 (耗时 0.043 秒)

    Scanning system and method for imaging and sequencing
    31.
    发明授权
    Scanning system and method for imaging and sequencing 有权
    扫描系统和成像和测序方法

    公开(公告)号：US08834797B2

    公开(公告)日：2014-09-16

    申请号：US12384516

    申请日：2009-04-06

    申请人： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

    发明人： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

    IPC分类号： C12Q1/68 ,  G01N33/52

    CPC分类号： G01N21/6486 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456

    摘要： A scanning detection system is provided in which emissions from locations in a flow cell are detected. In some embodiments, the system can include an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

    摘要翻译： 提供了一种扫描检测系统，其中检测到流动池中的位置的发射。 在一些实施例中，系统可以包括激发源，光切割源和调制光学器件，其被配置为使得由激发源产生的激发光束照射第一组固定位置并引起由光切割源产生的光切割束 以照射与第一组固定位置分开并分开的第二组固定位置。 还提供了使用这种系统检测测序反应的方法。

    METHODS, SYSTEMS AND APPARATUS FOR LIGHT CONCENTRATING MECHANISMS
    32.
    发明申请
    METHODS, SYSTEMS AND APPARATUS FOR LIGHT CONCENTRATING MECHANISMS 审中-公开
    用于光浓缩机制的方法，系统和装置

    公开(公告)号：US20080066549A1

    公开(公告)日：2008-03-20

    申请号：US11857419

    申请日：2007-09-18

    申请人： Mark F. OLDHAM ,  Eric S. Nordman ,  Charles R. Connell ,  Timothy M. Woudenberg

    发明人： Mark F. OLDHAM ,  Eric S. Nordman ,  Charles R. Connell ,  Timothy M. Woudenberg

    IPC分类号： G01N33/00

    CPC分类号： G01N21/648 ,  G01N2021/6432

    摘要： An embodiment relates generally to a method for analysis of a nucleic acid. The method includes providing for a resonant structure configured to couple with one or more fluorescently labeled nucleic acids and directing an excitation light from a source on the resonant structure. The method also includes generating plasmons on the surface of the resonant structure where the analyte is fixed at a point of energy concentration of the resonant structure.

    摘要翻译： 实施方案一般涉及核酸分析方法。 该方法包括提供构造成与一个或多个荧光标记的核酸耦合并将来自源的激发光引导到谐振结构上的谐振结构。 该方法还包括在谐振结构的表面上产生等离子体激元，其中分析物固定在谐振结构的能量集中点处。

    Time-delay integration in electrophoretic detection systems
    36.
    发明授权
    Time-delay integration in electrophoretic detection systems 有权

    公开(公告)号：US06856390B2

    公开(公告)日：2005-02-15

    申请号：US10205028

    申请日：2002-07-25

    申请人： Eric S. Nordman ,  Richard T. Reel ,  John Shigeura

    发明人： Eric S. Nordman ,  Richard T. Reel ,  John Shigeura

    IPC分类号： G01N21/64 ,  G01N27/447 ,  G01N21/00

    CPC分类号： G01N27/44721

    摘要： An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.

    Time-delay integration in electrophoretic detection systems
    38.
    发明授权
    Time-delay integration in electrophoretic detection systems 有权
    电泳检测系统中的时延整合

    公开(公告)号：US07468793B2

    公开(公告)日：2008-12-23

    申请号：US11012664

    申请日：2004-12-15

    申请人： Eric S. Nordman ,  Richard T. Reel ,  John S. Shigeura

    发明人： Eric S. Nordman ,  Richard T. Reel ,  John S. Shigeura

    IPC分类号： G01N21/00

    CPC分类号： G01N27/44721

    摘要： An apparatus for detecting analytes in a sample is provided. The apparatus includes: one or more channels having a detection zone; one or more irradiation sources disposed for irradiating the detection zone with non-coherent radiation; a detector array disposed for collecting light signals emitted from markers in the detection zone excited by the radiation, the detector array having an output; and a system coupled to the detector array for effecting time delay integration of the charges on the detector array corresponding to the light signals by accumulating the charges before reading the charges at the output of the detector array. Other apparatus and methods for detecting analytes in a sample are also provided.

    摘要翻译： 提供了一种用于检测样品中分析物的装置。 该装置包括：具有检测区域的一个或多个通道; 设置用于以非相干辐射照射检测区域的一个或多个照射源; 检测器阵列，被设置用于收集由所述辐射激发的所述检测区域中的标记物发出的光信号，所述检测器阵列具有输出; 以及耦合到检测器阵列的系统，用于通过在读取检测器阵列的输出端的电荷之前累积电荷来实现对与光信号对应的检测器阵列上的电荷的时间延迟积分。 还提供了用于检测样品中分析物的其它装置和方法。

    DNA sequencing methods and detectors and systems for carrying out the same
    40.
    发明授权
    DNA sequencing methods and detectors and systems for carrying out the same 有权
    DNA测序方法和检测器和系统进行相同

    公开(公告)号：US08969090B2

    公开(公告)日：2015-03-03

    申请号：US12984269

    申请日：2011-01-04

    申请人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    发明人： Hongye Sun ,  Eric S. Nordman ,  Mark F. Oldham ,  John R. O'Neill ,  Charles Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theofilos Kotseroglou ,  Kenneth J. Livak

    IPC分类号： G01N27/447 ,  G01N33/543 ,  C12Q1/68 ,  G01N27/414 ,  G01Q60/24 ,  C08G69/42 ,  B01L3/00

    CPC分类号： C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

    摘要： In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

    摘要翻译： 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极布置以及设置在纳米通道中的多个纳米器件器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔是制备它们的方法。 在一些实施方案中，提供了用于减缓通过纳米孔的单链DNA（ssDNA）易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。