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    REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY
    34.
    发明申请
    REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY 有权
    具有改善热稳定性的反向转录酶

    公开(公告)号：US20140045244A1

    公开(公告)日：2014-02-13

    申请号：US13984746

    申请日：2012-02-07

    申请人： Han Oh Park ,  Sung Jun Yang ,  Sung Mo Joo ,  Byoung Oh Hwang

    发明人： Han Oh Park ,  Sung Jun Yang ,  Sung Mo Joo ,  Byoung Oh Hwang

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1276 ,  C12N1/20 ,  C12N9/12 ,  C12N15/70 ,  C12P19/34 ,  Y02P20/52

    摘要： The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.

    摘要翻译： 本发明涉及具有改善的热稳定性的逆转录酶，更准确地说，具有通过取代选自第63位谷氨酰胺（Q63），第264位赖氨酸（K264）， 第309位赖氨酸（K295），第306位苏氨酸（T306），第346位谷氨酸（E346），第408位脯氨酸（P408），第438位组氨酸（H438）和第454位天门冬酰胺（N454） MLV起源于SEQ ID NO： ID。 NO：1与其他氨基酸。 与野生型逆转录酶相比，本发明的突变型逆转录酶表现出优异的热稳定性。 因此，即使在能够在高温下形成稳定的二级结构的RNA的存在下，也可以获得具有稳定的逆转录活性的靶cDNA。

    AUTOMATIC NECLEIC ACID PURIFICATION APPARATUS AND METHOD FOR AEROSOL-PROTECTING
    35.
    发明申请
    AUTOMATIC NECLEIC ACID PURIFICATION APPARATUS AND METHOD FOR AEROSOL-PROTECTING 有权
    自动酸性净化装置及其保护方法

    公开(公告)号：US20130236903A1

    公开(公告)日：2013-09-12

    申请号：US13885556

    申请日：2011-11-18

    申请人： Han Oh Park ,  Byung Rae Jeong ,  Kwon Sic Kim

    发明人： Han Oh Park ,  Byung Rae Jeong ,  Kwon Sic Kim

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1013 ,  C12Q1/68

    摘要： Disclosed is an automatic nucleic acid purification apparatus which can prevent pollution due to aerosol generated from a biological sample containing high concentration target nucleic acid when the biological sample containing the high concentration target nucleic acid is mixed with other biological sample containing low concentration target nucleic acid or not containing the target nucleic acid. Further, disclosed is an automatic nucleic acid purification apparatus which can be applied to all kinds of nucleic acid purification equipments for purifying a plurality of biological samples using a magnet rode or a multi-pipette block moving in two or three axial directions, and which can minimize pollution due to the aerosol generated from the biological sample containing high concentration target nucleic acid and also can obtain accurate results.

    摘要翻译： 公开了一种自动核酸纯化装置，当含有高浓度靶核酸的生物样品与含有低浓度靶核酸的其他生物样品混合时，可以防止由含有高浓度靶核酸的生物样品产生的气溶胶引起的污染，或 不含靶核酸。 此外，公开了一种自动核酸纯化装置，其可以应用于使用磁珠或多个移液管块在两个或三个轴向移动的多个生物样品的各种核酸纯化设备，并且其可以 尽可能减少由含有高浓度靶核酸的生物样品产生的气溶胶引起的污染，并可获得准确的结果。

    Lactic acid bacteria isolated from mother's milk with probiotic activity and inhibitory activity against body weight augmentation
    37.
    发明授权
    Lactic acid bacteria isolated from mother's milk with probiotic activity and inhibitory activity against body weight augmentation 有权
    从母乳分离的乳酸菌具有益生菌活性和对体重增加的抑制活性

    公开(公告)号：US08309076B2

    公开(公告)日：2012-11-13

    申请号：US12376368

    申请日：2007-05-14

    申请人： Ji Hee Kang ,  Byeung Il You ,  Sung Il Yun ,  Han Oh Park

    发明人： Ji Hee Kang ,  Byeung Il You ,  Sung Il Yun ,  Han Oh Park

    IPC分类号： A61K35/74 ,  C12N1/20

    CPC分类号： C12R1/225 ,  A23C9/1234 ,  A23L33/135 ,  A23L33/18 ,  A23Y2220/37 ,  A61K38/00 ,  C07K14/335 ,  C12N1/20

    摘要： The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body. Therefore, the strain of the invention, owing to such beneficiary effects, can be effectively used not only for the production of fermented milk, other fermented food products and animal feeds but also for the production of live cell products and food additives for preventing weight gaining.

    摘要翻译： 本发明涉及从人母乳分离的乳酸菌，更准确地说是从韩国母乳中分离的加氏乳杆菌BNR17菌株，具有优异的益生菌活性，包括耐酸性，耐胆汁酸性和抗微生物活性和增重抑制作用 好。 此外，本发明的加氏乳杆菌BNR17除了通过从包含在食物中的单糖合成难消化的多糖而获得的重量增加抑制作用外，还具有优异的耐酸性，耐胆汁酸性，肠吸收活性和抗病原微生物的抗微生物活性， 合成多糖出体外。 因此，由于这种受益效应，本发明的菌株不仅可以有效地用于生产发酵乳，其他发酵食品和动物饲料，而且可用于生产活细胞产品和食品添加剂，以防止体重增加 。

    SAMPLE PRECONCENTRATOR
    38.
    发明申请
    SAMPLE PRECONCENTRATOR 有权
    样品预处理器

    公开(公告)号：US20120216597A1

    公开(公告)日：2012-08-30

    申请号：US13504815

    申请日：2010-03-31

    申请人： Han Oh Park ,  Yang-Won Lee ,  Young-Mi Koo ,  Kwang-Woo Jung

    发明人： Han Oh Park ,  Yang-Won Lee ,  Young-Mi Koo ,  Kwang-Woo Jung

    IPC分类号： G01N1/40 ,  B82Y35/00

    CPC分类号： G01N1/405 ,  G01N35/1097

    摘要： There is provided a sample preconcentrator. The sample preconcentrator in which a sample gas injection port is coupled to a dried gas supply source and a gas analysis system to concentrate a sample gas comprises a sample concentrating unit containing an absorbent that is composed of carbon nanotube-metal nanocomplexes; a conduit switching valve for selectively coupling the sample gas injection port to the dried gas supply source and the gas analysis system and controlling the absorption and desorption of the sample gas from the sample concentrating unit; and a plurality of conduits for connecting the sample gas injection port, the dried gas supply source, the gas analysis system, the sample concentrating unit and the conduit switching valve.

    摘要翻译： 提供了一个样本预浓缩器。 样品预浓缩器，其中样品气体注入口连接到干燥气体供应源和用于浓缩样品气体的气体分析系统包括含有由碳纳米管 - 金属纳米复合物构成的吸收剂的样品浓缩单元; 用于选择性地将样品气体注入口连接到干燥气体供应源和气体分析系统并控制来自样品浓缩单元的样品气体的吸收和解吸的导管切换阀; 以及用于连接样品气体注入口，干燥气体供给源，气体分析系统，样品浓缩单元和导管切换阀的多个导管。