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公开(公告)号：US07306917B2
公开(公告)日：2007-12-11
申请号：US11222565
申请日：2005-09-09
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。
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公开(公告)号：US06214545B1
公开(公告)日：2001-04-10
申请号：US08851588
申请日：1997-05-05
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133 , C12Q2565/501
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US5422253A
公开(公告)日：1995-06-06
申请号：US986330
申请日：1992-12-07
IPC分类号： C07H21/00 , C07K14/47 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , G01N33/53 , C12P19/34
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6853 , C12Q1/686 , C12Q1/705
摘要： A method of cleaving a target nucleic acid molecule is disclosed. A cleavage structure is formed that comprises the target nucleic acid and a pilot nucleic acid. A first region of the target nucleic acid is annealed to the pilot nucleic acid to form a duplex structure. A second region of the target nucleic acid contiguous to the duplex is not annealed to the pilot nucleic acid, thus forming a junction site between the duplex region and the non-annealed region. The cleavage structure is exposed to a cleavage agent capable of preferentially cleaving the cleavage structure at a target site in a manner independent of the sequence of the cleavage structure. The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
摘要翻译： 公开了切割靶核酸分子的方法。 形成包含靶核酸和导向核酸的切割结构。 靶核酸的第一区域与引导核酸退火以形成双链体结构。 与双链体相邻的靶核酸的第二区域不与引导核酸退火，从而在双相区域和非退火区域之间形成连接位点。 裂解结构暴露于能够以与切割结构的序列无关的方式在靶位点优先切割切割结构的切割剂。 裂解结构和切割剂在可以发生切割的条件下孵育。
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公开(公告)号：US06780585B1
公开(公告)日：2004-08-24
申请号：US09676768
申请日：2000-10-02
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133 , C12Q2565/501
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
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公开(公告)号：US20080187926A1
公开(公告)日：2008-08-07
申请号：US11929878
申请日：2007-10-30
申请人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的改进方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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36.发明授权Method of inhibition of nucleo-cytoplasmic transport by M protein of vesicular stomatitis virus 失效抑制水泡性口炎病毒M蛋白核 - 细胞质运输的方法
公开(公告)号：US5888727A
公开(公告)日：1999-03-30
申请号：US861104
申请日：1997-05-21
申请人： Elsebet Lund , James E. Dahlberg , Lu-Shiun Her , Yan Cheng , Christian Grimm
发明人： Elsebet Lund , James E. Dahlberg , Lu-Shiun Her , Yan Cheng , Christian Grimm
CPC分类号： C12N15/1086 , C07K14/005 , C12N15/11 , C12N15/63 , C12N2760/20222
摘要： A method of inhibiting transport of nucleic acids between the nucleus and cytoplasm of a cell is disclosed. In one embodiment, this method comprises the step of exposing a cell to a sufficient amount of vesicular stomatitis virus M protein such that the transport of RNA and protein through the nuclear envelope of the cell is impeded. A chimeric RNA capable of nuclear export is also disclosed.
摘要翻译： 公开了一种抑制核酸在细胞核和细胞质之间的转运的方法。 在一个实施方案中，该方法包括将细胞暴露于足够数量的水泡性口炎病毒M蛋白的步骤，使得RNA和蛋白质通过细胞核包膜的转运受到阻碍。 还公开了能够进行核出口的嵌合RNA。
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公开(公告)号：US20120149018A1
公开(公告)日：2012-06-14
申请号：US13368191
申请日：2012-02-07
申请人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T. Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn C. Olson-Munoz , LuAnne Chehak , Sarah M. Olson
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to improved methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的改进方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US08609336B2
公开(公告)日：2013-12-17
申请号：US13368191
申请日：2012-02-07
申请人： James E. Dahlberg , Hatim T Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah M. Olson
发明人： James E. Dahlberg , Hatim T Allawi , Victor Lyamichev , Bruce P. Neri , Marilyn Olson-Munoz , LuAnne Chehak , Sarah M. Olson
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/1015 , C12Q2561/109 , C12Q2525/207
摘要： The present invention relates to compositions and methods for the detection and characterization of interfering RNAs such as micro RNAs (miRNAs) and small interfering RNAs (siRNAs) and other short nucleic acid molecules. More particularly, the present invention relates to methods for the detection and quantitation of interfering RNA expression. The present invention further provides for the detection of variants and types of miRNAs and siRNAs.
摘要翻译： 本发明涉及用于检测和表征干扰RNA如微RNA（miRNA）和小干扰RNA（siRNA）和其他短核酸分子的组合物和方法。 更具体地，本发明涉及用于检测和定量干扰RNA表达的方法。 本发明还提供了miRNA和siRNA的变体和类型的检测。
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公开(公告)号：US07700288B2
公开(公告)日：2010-04-20
申请号：US11352837
申请日：2006-02-13
申请人： James E. Dahlberg , Peggy S. Eis , Wayne Tam , Elsebet Lund
发明人： James E. Dahlberg , Peggy S. Eis , Wayne Tam , Elsebet Lund
IPC分类号： C12Q1/68
CPC分类号： G01N33/5052 , C12Q1/6886 , C12Q2600/112 , C12Q2600/136 , C12Q2600/178 , G01N33/5091 , G01N33/57426
摘要： The invention provides methods for diagnosing B-cell lymphoma in an animal. In particular, the invention provides methods for distinguishing an animal having diffuse large B-cell lymphoma (DLBCL) with an activated B-cell (ABC) phenotype from an animal having DLBCL with a non-activated germinal-center (GC) phenotype. The invention also provides methods for identifying compounds for treating B-cell lymphoma. The invention further provides reagents and methods for determining the amount of miR-155 in sample isolated from an animal. In this regard, the invention provides a set of oligonucleotides for determining the amount of miR-155 in sample isolated from an animal.
摘要翻译： 本发明提供了用于诊断动物中B细胞淋巴瘤的方法。 特别地，本发明提供了从具有非活化生发中心（GC）表型的DLBCL的动物区分具有弥漫性大B细胞淋巴瘤（DLBCL）的动物与活化的B细胞（ABC）表型的方法。 本发明还提供鉴定用于治疗B细胞淋巴瘤的化合物的方法。 本发明还提供用于测定从动物分离的样品中miR-155的量的试剂和方法。 在这方面，本发明提供了一组用于确定从动物分离的样品中miR-155的量的寡核苷酸。
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40.发明授权Inhibition of nucleocytoplasmic transport by vesicular stomatitis virus M protein-like polypeptides 失效抑制水泡性口炎病毒M蛋白样多肽的核细胞质转运公开(公告)号：US06653103B2
公开(公告)日：2003-11-25
申请号：US10112802
申请日：2002-03-29
IPC分类号： C12P2106
CPC分类号： G01N33/5076 , A61K38/00 , C07K14/005 , C07K2319/00 , C07K2319/09 , C12N2760/20222 , C12N2760/20233
摘要： A fragment of vesicular stomatitis virus (VSV) matrix protein (M protein) and M proteins of other viral species that can inhibit nucleocytoplasmic transport of RNA, proteins and RNA-protein complexes are disclosed. These polypeptide products and related polypeptides can be used to inhibit nucleocytoplasmic transport. Further disclosed are fragments of the VSV M protein that can enter into the nucleus of a cell. These fragments and the full length of the VSV M protein can be used to introduce other polypeptides into the nucleus of a cell.
摘要翻译： 公开了可抑制RNA，蛋白质和RNA-蛋白复合物的核胞质转运的其他病毒物种的水泡性口炎病毒（VSV）基质蛋白（M蛋白）和M蛋白的片段。 这些多肽产物和相关多肽可用于抑制核细胞质转运。 进一步公开的是能够进入细胞核的VSV M蛋白的片段。 这些片段和全长的VSV M蛋白可用于将其他多肽引入细胞核。
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