摘要:
The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for seed-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the seed-preferred regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要:
Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要:
Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要:
The invention provides isolated nucleic acids and their encoded proteins that act as cell death inhibitors and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.
摘要:
A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
摘要:
Compositions and methods relating to the use of tetracycline repressor in plants are provided. Compositions include a polynucleotide modified for expression in a plant, wherein the polynucleotide encodes a tetracycline repressor protein, as well as constructs, vectors, cells, plants and seeds comprising the polynucleotide, an/or produced by the methods. Also provided are methods to provide tetracycline repressor to a cell, and to regulate expression of a polynucleotide of interest in a cell, including a plant cell.
摘要:
Compositions and methods relating to the use of tetracycline repressor in plants are provided. Compositions include a polynucleotide modified for expression in a plant, wherein the polynucleotide encodes a tetracycline repressor protein, as well as constructs, vectors, cells, plants and seeds comprising the polynucleotide, an/or produced by the methods. Also provided are methods to provide tetracycline repressor to a cell, and to regulate expression of a polynucleotide of interest in a cell, including a plant cell.
摘要:
Compositions and methods relating to the use of sulfonylurea-mediated control of gene expression are provided. Compositions include sulfonylurea responsive chemical switches wherein the gene expression is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to regulate expression of a polynucleotide of interest in a cell or organism, and methods to modify a genome, including in a plant or plant cell.
摘要:
Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译:提供了调节植物发育的方法和组合物。 提供编码Ovule Development Protein 2(ODP2)蛋白的核苷酸序列和氨基酸序列。 这些序列可以用于各种方法,包括调节发育,发育途径,改变植物中的油含量,提高转化效率,调节胁迫耐受性和调节植物的再生能力。 还提供了转化的植物,植物细胞,组织和种子。
摘要:
Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译:提供了调节植物发育的方法和组合物。 提供编码Ovule Development Protein 2(ODP2)蛋白的核苷酸序列和氨基酸序列。 这些序列可以用于各种方法,包括调节发育,发育途径,改变植物中的油含量,提高转化效率,调节胁迫耐受性和调节植物的再生能力。 还提供了转化的植物,植物细胞,组织和种子。