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公开(公告)号：US20070136840A1
公开(公告)日：2007-06-14
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl Peters , Jill Hinson , Philip Hammer , Brian Vande Berg , Laura Schouten , Brian Carr
发明人： Cheryl Peters , Jill Hinson , Philip Hammer , Brian Vande Berg , Laura Schouten , Brian Carr
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。 本发明另外提供了使用荧光底物测量酶动力学活性的方法。
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公开(公告)号：US09045765B2
公开(公告)日：2015-06-02
申请号：US11760570
申请日：2007-06-08
申请人： Brian Vande Berg , Cheryl Peters , Brian Carr , Daniel John Tomso
发明人： Brian Vande Berg , Cheryl Peters , Brian Carr , Daniel John Tomso
CPC分类号： C12N15/8275 , C12N9/1092 , C12N15/8261
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS:2, 4, 6, 8, 10, 12, or 14 or the nucleotide sequence set forth in SEQ ID NOS:1, 3, 5, 7, 9, 11, 13, 28, 29, 30, 31, 32, 33, or 34.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：2,4,6,8,10,12或14所示氨基酸序列的核苷酸序列或SEQ ID NOS ：1，3，5，7，9，11，13，28，29，30，31，32，33或34。
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公开(公告)号：US08692070B2
公开(公告)日：2014-04-08
申请号：US12916854
申请日：2010-11-01
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10 , C12N1/21 , C12N15/00 , C12N15/29 , C12N15/54 , C07H21/04
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻增加产量的限制的转基因植物，或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物 谷氨酸激酶（NAGK）基因，调节植物中的氮利用。 本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。 在各种实施方案中，载体构建体包括一个或多个包含SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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34.发明授权公开(公告)号：US08309332B2
公开(公告)日：2012-11-13
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。 本发明另外提供了使用荧光底物测量酶动力学活性的方法。
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公开(公告)号：US08252980B2
公开(公告)日：2012-08-28
申请号：US12856021
申请日：2010-08-13
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 31, or the nucleotide sequence set forth in SEQ ID NO: 30.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了含有编码SEQ ID NO：31所示的氨基酸序列的核苷酸序列或SEQ ID NO：30所示的核苷酸序列的分离的多核苷酸。
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36.发明授权公开(公告)号：US08003854B2
公开(公告)日：2011-08-23
申请号：US11769255
申请日：2007-06-27
申请人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
发明人： Cheryl Peters , Brian Vande Berg , Brian Carr , Daniel John Tomso
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include nucleic acid molecules encoding herbicide resistance or tolerance polypeptides, vectors comprising those nucleic acid molecules, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, the present invention provides for isolated nucleic acid molecules comprising the nucleotide sequence set forth in SEQ ID NO:1 or 14, a nucleotide sequence encoding the amino acid sequence shown in SEQ ID NO:2, the herbicide resistance nucleotide sequence deposited in a bacterial host as Accession Nos. NRRL B-30931, as well as variants and fragments thereof.
摘要翻译： 提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的核酸分子，包含那些核酸分子的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，本发明提供了分离的核酸分子，其包含SEQ ID NO：1或14所示的核苷酸序列，编码SEQ ID NO：2所示氨基酸序列的核苷酸序列， 作为登录号NRRL B-30931的细菌宿主，以及其变体和片段。
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37.发明授权公开(公告)号：US07989679B2
公开(公告)日：2011-08-02
申请号：US11681285
申请日：2007-03-02
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译： 提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。 组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。 还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 此外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。 还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。
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公开(公告)号：US20100166723A1
公开(公告)日：2010-07-01
申请号：US12638591
申请日：2009-12-15
申请人： Cheryl L. Peters , Brian Vande Berg , Brian Carr , Julia T. Daum , Vadim Beilinson , Sandra Volrath , Candace Poutre , Kimberly Sampson , Theodore Kahn
发明人： Cheryl L. Peters , Brian Vande Berg , Brian Carr , Julia T. Daum , Vadim Beilinson , Sandra Volrath , Candace Poutre , Kimberly Sampson , Theodore Kahn
CPC分类号： C12N15/8241 , C12N9/0059 , C12N9/0071 , C12N15/8285 , C12Y110/03001 , C12Y114/18001 , Y02A40/164
摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.
摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。 提供了包含杀线虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀线虫剂核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列 在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。
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公开(公告)号：US07674958B2
公开(公告)日：2010-03-09
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。 本发明另外提供了使用荧光底物测量酶动力学活性的方法。
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公开(公告)号：US20090227771A1
公开(公告)日：2009-09-10
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C07K14/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。 本发明另外提供了使用荧光底物测量酶动力学活性的方法。
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