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公开(公告)号：US20110252503A1
公开(公告)日：2011-10-13
申请号：US12916854
申请日：2010-11-01
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻增加产量的限制的转基因植物，或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物 谷氨酸激酶（NAGK）基因，调节植物中的氮利用。 本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。 在各种实施方案中，载体构建体包括一个或多个包含SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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公开(公告)号：US08692070B2
公开(公告)日：2014-04-08
申请号：US12916854
申请日：2010-11-01
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10 , C12N1/21 , C12N15/00 , C12N15/29 , C12N15/54 , C07H21/04
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻增加产量的限制的转基因植物，或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物 谷氨酸激酶（NAGK）基因，调节植物中的氮利用。 本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。 在各种实施方案中，载体构建体包括一个或多个包含SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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公开(公告)号：US20120144520A1
公开(公告)日：2012-06-07
申请号：US13309308
申请日：2011-12-01
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。 在各种实施方案中，载体构建体包含选自SEQ ID NO： 34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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公开(公告)号：US20100162433A1
公开(公告)日：2010-06-24
申请号：US11977768
申请日：2007-10-26
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Alissa Schawalder , Vadim Beilinson , Jill Hinson
CPC分类号： C12N15/8271 , C12N15/8216 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译： 本发明涉及具有增加的氮利用效率，胁迫耐受性或两者的转基因植物，并且已经使用包含调节植物中氮利用的核酸序列的新型载体构建体进行了转化。 在各种实施方案中，载体构建体包含一个或多个选自SEQ ID NO： 32,34,36或38.本发明还涉及用于转化植物的分离载体和用于检测转化植物的抗体。 本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。
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5.发明申请ELECTROCHEMICAL-BASED ANALYTICAL TEST STRIP WITH FILL-SPEED CONFIGURED REAGENT LAYER 审中-公开基于电化学的分析测试条带快速配置的试剂层公开(公告)号：US20130199942A1
公开(公告)日：2013-08-08
申请号：US13367648
申请日：2012-02-07
申请人： Neil WHITEHEAD , Stuart Phillips , David Morris , Joanne McIlrath , Robert MacLeod , Lynsey Whyte , Karn Campbell , Ramsay Darling , James McLaren , Russell Bain
发明人： Neil WHITEHEAD , Stuart Phillips , David Morris , Joanne McIlrath , Robert MacLeod , Lynsey Whyte , Karn Campbell , Ramsay Darling , James McLaren , Russell Bain
IPC分类号： G01N27/327 , G01N33/66
摘要： An electrochemical-based analytical test strip (“EBAT”) for the determination of an analyte in a bodily fluid sample includes an electrically insulating substrate layer with a distal end and a patterned conductor layer that is disposed over the electrically-insulating substrate layer and has a working electrode (“WE”) and a counter/reference electrode (“C/RE”). The EBAT also includes a patterned insulation layer with an electrode exposure window configured to expose a WE exposed portion and a C/RE exposed portion, an enzymatic reagent layer; and a patterned spacer layer. The patterned insulation layer and the patterned spacer layer define a sample receiving chamber with a sample-receiving opening (“SRO”) at the distal end of the electrically insulating substrate layer and that extends across the WE exposed portion and the C/RE exposed portion. Furthermore, the enzymatic reagent layer is disposed over the WE and C/RE exposed portions and extends no more than 400 μm toward the SRO.
摘要翻译： 用于测定体液样品中的分析物的基于电化学的分析测试条（“EBAT”）包括具有远端的电绝缘基底层和设置在电绝缘基底层上的图案化导体层，并且具有 工作电极（“WE”）和计数器/参考电极（“C / RE”）。 EBAT还包括具有电极曝光窗口的图案化绝缘层，其被配置为暴露WE暴露部分和C / RE暴露部分，即酶试剂层; 和图案化间隔层。 图案化绝缘层和图案化间隔层限定了在电绝缘基底层的远端处具有样品接收开口（“SRO”）的样品接收室，并且延伸穿过WE暴露部分和C / RE暴露部分 。 此外，酶试剂层设置在WE和C / RE暴露部分上方，朝向SRO延伸不超过400μm。
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公开(公告)号：US4101413A
公开(公告)日：1978-07-18
申请号：US771314
申请日：1977-02-23
IPC分类号： C10G1/04
CPC分类号： C10G1/04 , Y10S585/943
摘要： Coal can be extracted with high boiling aromatic oils produced by extracting the aromatic constituents of a heavy petroleum material, e.g. a bitumen residue, using a selective solvent, mixing the extract with a low-boiling aromatic solvent, adding water and separating the aromatic-containing phase and recovering said aromatic constituents. The resulting coal extract is particularly suitable for the production of electrode coke.
摘要翻译： 煤可以用通过提取重质石油材料的芳族成分制备的高沸点芳香油提取，例如， 沥青残余物，使用选择性溶剂，将提取物与低沸点芳族溶剂混合，加入水并分离含芳族相并回收所述芳族成分。 所得到的煤提取物特别适用于生产电极焦炭。
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