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公开(公告)号：US4954618A
公开(公告)日：1990-09-04
申请号：US280963
申请日：1989-03-15
IPC分类号： C07K14/315 , C12N15/31 , C12N15/70 , C12N15/75 , C12P21/02
CPC分类号： C12N15/70 , C07K14/315 , C12N15/75 , C12P21/02 , Y10S530/811 , Y10S530/861
摘要： A cloned gene encoding Protein G, or functionally active portions thereof, vectors containing the cloned gene, and microorganisms transformed by those vectors are disclosed.
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公开(公告)号：US07427656B2
公开(公告)日：2008-09-23
申请号：US11516362
申请日：2006-09-05
CPC分类号： A61K8/64 , A61K2800/94 , A61Q3/00 , A61Q5/10 , A61Q17/005 , A61Q17/04 , A61Q19/00 , A61Q19/04 , C07K2319/00
摘要： Peptide tags, referred to here as inclusion body tags, are disclosed and are useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
摘要翻译： 肽标签，这里称为包涵体标签，并且可用于产生不溶性融合肽。 融合肽包含可操作地连接到目的肽的至少一种包涵体标签。 融合肽在宿主细胞中的表达产生不溶性并且包含在细胞和/或细胞裂解物中的包涵体内的产物。 然后可以纯化包涵体，并且在从包涵体标签切割后可以分离感兴趣的蛋白质。
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33.发明申请Zein-based peptide tags for the expression and purification of bioactive peptides 失效用于表达和纯化生物活性肽的基于Zein的肽标签公开(公告)号：US20080096246A1
公开(公告)日：2008-04-24
申请号：US11641936
申请日：2006-12-19
CPC分类号： C07K14/425 , C07K2319/00
摘要： Zein-based peptide tags, referred to here as inclusion body tags (IBTs), are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
摘要翻译： 公开了用于产生不溶性融合肽的基于Zein的肽标签，这里称为包涵体标签（IBT）。 融合肽包含可操作地连接到目的肽的至少一种包涵体标签。 融合肽在宿主细胞中的表达产生不溶性并且包含在细胞和/或细胞裂解物中的包涵体内的产物。 然后可以纯化包涵体，并且在从包涵体标签切割后可以分离感兴趣的蛋白质。
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34.发明申请Cystatin-based peptide tags for the expression and purification of bioactive peptides 失效用于表达和纯化生物活性肽的基于半胱氨酸蛋白酶抑制剂的肽标签公开(公告)号：US20080096245A1
公开(公告)日：2008-04-24
申请号：US11641273
申请日：2006-12-19
CPC分类号： C07K14/4702 , C07K2319/00
摘要： Cystatin-based peptide tags, referred to here as inclusion body tags (IBTs), are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.
摘要翻译： 公开了用于产生不溶性融合肽的基于半胱氨酸蛋白酶抑制剂的肽标签，这里称为包涵体标签（IBT）。 融合肽包含可操作地连接到目的肽的至少一种包涵体标签。 融合肽在宿主细胞中的表达产生不溶性并且包含在细胞和/或细胞裂解物中的包涵体内的产物。 然后可以纯化包涵体，并且在从包涵体标签切割后可以分离感兴趣的蛋白质。
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公开(公告)号：US06268169B1
公开(公告)日：2001-07-31
申请号：US08556978
申请日：1995-12-11
IPC分类号： C12N121
CPC分类号： C07K14/43518 , F02B2075/027
摘要： The invention relates to novel spider silk protein analogs derived from the amino acid consensus sequence of repeating units found in the natural spider dragline of Nephila clavipes. More specifically, synthetic spider dragline has been produced from E. coli and Bacillus subtilis recombinant expression systems wherein expressions from E. coli is at levels greater than 1 mg full-length polypeptide per gram of cell mass.
摘要翻译： 本发明涉及衍生自Nephila clavipes天然蜘蛛牵引线中重复单元的氨基酸共有序列的新型蜘蛛丝蛋白类似物。 更具体地，已经从大肠杆菌和枯草芽孢杆菌重组表达系统产生了合成蜘蛛牵引线，其中来自大肠杆菌的表达水平每克细胞质量大于1mg全长多肽。
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36.发明授权Cloned streptococcal genes encoding protein G and their use to construct recombinant microorganisms to produce protein G 失效编码蛋白G的克隆链球菌基因及其用于构建重组微生物以产生蛋白G的用途
公开(公告)号：US5229492A
公开(公告)日：1993-07-20
申请号：US540169
申请日：1990-06-19
IPC分类号： C07K14/315 , C12N15/70 , C12N15/75
CPC分类号： C07K14/315 , C12N15/70 , C12N15/75
摘要： Protein G variants are disclosed. These protein G variants have the immunoglobulin binding properties of protein G. Also disclosed is the preparation of the protein G variants by expression from hosts transformed with a gene encoding the protein G variants.
摘要翻译： 公开了蛋白G变体。 这些蛋白G变体具有蛋白G的免疫球蛋白结合特性。还公开了通过用编码蛋白G变体的基因转化的宿主的表达来制备蛋白G变体。
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公开(公告)号：US4828994A
公开(公告)日：1989-05-09
申请号：US733379
申请日：1985-05-13
CPC分类号： C12N9/54 , C12N15/75 , C12N15/90 , Y10S435/839
摘要： Bacillus strains having reduced levels of extracellular protease are produced by replacing the native chromosomal DNA sequence comprising the gene for an extracellular protease, such as subtilisin, with a partially homologous DNA sequence having an inactivating DNA segment inserted therein. The strains are useful as hosts for the expression and secretion of heterologous polypeptides or proteins.
摘要翻译： 通过用包含插入其中的失活DNA片段的部分同源DNA序列替换包含细胞外蛋白酶基因例如枯草杆菌蛋白酶的天然染色体DNA序列来产生细胞外蛋白酶水平降低的芽孢杆菌菌株。 该菌株可用作异源多肽或蛋白质的表达和分泌的宿主。
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公开(公告)号：US08287845B2
公开(公告)日：2012-10-16
申请号：US12629069
申请日：2009-12-02
摘要： Hair-binding peptides were isolated for their use in a variety of personal care formulations and applications. The isolation of hair-binding peptides was accomplished by enrichment using mRNA-display selection technology. Hair care compositions comprising peptide-based reagents prepared comprising the hair-binding peptides are also provided.
摘要翻译： 分离发粘结肽用于各种个人护理制剂和应用。 通过使用mRNA显示选择技术的富集来实现毛发结合肽的分离。 还提供了包含由毛发结合肽制备的包含基于肽的试剂的护发组合物。
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39.发明授权Ketosteroid isomerase inclusion body tag engineered to be acid-resistant by replacing aspartates with glutamate 失效通过用谷氨酸取代天冬氨酸，工程化为耐酸的类固醇异构酶包含体标签公开(公告)号：US07829311B2
公开(公告)日：2010-11-09
申请号：US12172385
申请日：2008-07-14
CPC分类号： C12P21/02 , C07K2319/50 , C12N9/90
摘要： An acid-resistant peptide solubility tag (an “inclusion body tag”) is provided that is effective in producing peptides of interest in an insoluble form. Fusion peptide constructs comprising the inclusion body tag fused to a peptide of interest are provided. An acid cleavable peptide moiety separates the inclusion body tag from the peptide of interest so that acid hydrolysis can be used during subsequent processing steps to separate the tag from the desired peptide of interest. The present inclusion body tag's resistance to acid hydrolysis facilitates easier and cleaner separation of the peptide of interest after acid hydrolysis. Specifically, a ketosteroid isomerase-derived inclusion body tag is provided that has been engineered to be more resistant to acid hydrolysis.
摘要翻译： 提供了一种耐酸肽溶解性标签（“包含体标签”），其有效地生产不溶形式的感兴趣的肽。 提供了包含与感兴趣的肽融合的包含体标签的融合肽构建体。 可酸切割肽部分将包涵体标签与感兴趣的肽分开，使得在随后的加工步骤中可以使用酸水解，以将标签与期望的目标肽分离。 本发明的包涵体标签对酸水解的抗性促进了酸水解后感兴趣的肽的更容易和更清洁的分离。 具体地说，提供了一种经过工程改造以耐酸解作用的酮类异构酶衍生的包含体标签。
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40.发明申请DYED-HAIR-BINDING PEPTIDES AND PEPTIDE-BASED HAIR REAGENTS FOR PERSONAL CARE 失效用于个人护理的染发头发胶原和基于肽的头发试剂公开(公告)号：US20090074694A1
公开(公告)日：2009-03-19
申请号：US12198382
申请日：2008-08-26
CPC分类号： C07K14/001 , A61K8/64 , A61K2800/57 , A61Q5/065 , A61Q5/10 , A61Q5/12
摘要： Peptides have been identified that bind with high affinity to dyed hair. Peptide-based hair reagents formed by coupling a dyed-hair-binding peptide to a benefit agent are described. The peptide-based hair reagents include peptide-based hair conditioners and hair colorants. The peptide-based hair conditioners and hair colorants are comprised of a dyed-hair-binding peptide coupled to a hair conditioning agent or a coloring agent, respectively. The peptide-based hair reagents or the dyed-hair-binding peptides may also be used as a protective sealant to improve color retention of dyed hair.
摘要翻译： 已经鉴定出以高亲和力结合染色的头发的肽。 描述了通过将染色结合肽偶联到有益剂形成的基于肽的毛发试剂。 基于肽的毛发试剂包括基于肽的护发素和头发着色剂。 基于肽的护发素和毛发着色剂分别由与头发调理剂或着色剂偶联的染色结合肽组成。 基于肽的毛发试剂或染色结合肽也可以用作保护性密封剂，以改善染色头发的保色性。
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