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公开(公告)号：US06947849B2
公开(公告)日：2005-09-20
申请号：US10356349
申请日：2003-01-31
摘要： The invention relates to methods of identifying and characterizing properties of polymers to provide information about the polymer such as the charge of the polymer, the number and types or characteristics of units of the polymer and the sequence of the polymers. The invention also relates to methods of sequencing polymers such as nucleic acids, polypeptides and polysaccharides and methods for identifying a polysaccharide-protein interaction.
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公开(公告)号：US08173384B2
公开(公告)日：2012-05-08
申请号：US12509774
申请日：2009-07-27
申请人： Ram Sasisekharan , Ganesh Venkataraman , Zachary Shriver , Dongfang Liu , Mallikarjun Sundaram , Yiwei Qi
发明人： Ram Sasisekharan , Ganesh Venkataraman , Zachary Shriver , Dongfang Liu , Mallikarjun Sundaram , Yiwei Qi
IPC分类号： C12Q1/56
CPC分类号： G01N33/66 , A61K31/727 , C08B37/0075 , C12P19/26 , G01N33/86 , G01N2400/10 , G01N2400/40 , Y10T436/143333
摘要： The invention relates to methods and products for analyzing or processing a heparin sample. In one embodiment, the methods comprise contacting the sample with heparinase I, heparinase II and heparinase III, determining the amount of a signature component in the sample using a separation method, and making a determination about the sample based upon a comparison of the amount of the signature component in the sample to a reference database for a heparin.
摘要翻译： 本发明涉及用于分析或加工肝素样品的方法和产品。 在一个实施方案中，所述方法包括使样品与肝素酶I，肝素酶II和肝素酶III接触，使用分离方法确定样品中的特征成分的量，并且基于对样品的量 样本中的签名组件到肝素的参考数据库。
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33.发明授权公开(公告)号：US07951560B2
公开(公告)日：2011-05-31
申请号：US12616764
申请日：2009-11-11
申请人： James R. Myette , Zachary Shriver , Ganesh Venkataraman , Ram Sasisekharan , Maitland W. McLean
发明人： James R. Myette , Zachary Shriver , Ganesh Venkataraman , Ram Sasisekharan , Maitland W. McLean
CPC分类号： C12Y302/01056 , A61K38/00 , C07H21/04 , C12N9/2402 , C12Q1/34 , C12Y302/01166 , Y02A50/481
摘要： The invention relates to Δ4,5 glycuronidase, related compositions, and methods of use thereof.
摘要翻译： 本发明涉及＆amp; 4,5g糖醛酸糖苷酶，相关组合物及其使用方法。
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公开(公告)号：US07737692B2
公开(公告)日：2010-06-15
申请号：US12261000
申请日：2008-10-29
申请人： Ram Sasisekharan , Ganesh Venkataraman , Rahul Raman , Benito Casu , Giangiacomo Torri , Marco Guerrini
发明人： Ram Sasisekharan , Ganesh Venkataraman , Rahul Raman , Benito Casu , Giangiacomo Torri , Marco Guerrini
IPC分类号： G01V3/00
CPC分类号： G01N24/08 , Y10T436/143333
摘要： The invention relates to methods for analyzing polysaccharides. In particular, compositional and sequence information about the polysaccharides are derived. Some methods use NMR in conjunction with another experimental method, such as, capillary electrophoretic techniques for the analysis.
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公开(公告)号：US07575886B2
公开(公告)日：2009-08-18
申请号：US10386402
申请日：2003-03-11
IPC分类号： C12Q1/34 , A61K31/727 , C08B37/10
CPC分类号： G01N33/66 , G01N33/86 , G01N2400/10 , G01N2400/40 , Y10T436/143333 , Y10T436/17 , Y10T436/24
摘要： The invention relates to methods and products associated with analyzing and monitoring heterogeneous populations of sulfated polysaccharides. In particular therapeutic heparin products including low molecular weight heparin products and methods of analyzing and monitoring these products are described.
摘要翻译： 本发明涉及与分析和监测硫酸多糖的异质种群相关的方法和产品。 特别是包括低分子量肝素产品的治疗性肝素产品以及分析和监测这些产品的方法进行了描述。
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36.发明授权Rationally designed heparinases derived from heparinase I and II and methods of sequencing therewith 有权合理设计源自肝素酶I和II的肝素酶及其测序方法公开(公告)号：US07560106B2
公开(公告)日：2009-07-14
申请号：US11191144
申请日：2005-07-27
CPC分类号： A61K38/47 , C08B37/0078 , C12N9/88 , C12N9/99
摘要： Modified heparinases having altered binding specificity and activity are provided. Isolated nucleic acids encoding the same as well as vectors and host cells are provided. Methods for using the modified heparinases are also provided.
摘要翻译： 提供具有改变的结合特异性和活性的修饰的肝素酶。 提供编码相同的分离的核酸以及载体和宿主细胞。 还提供了使用改性肝素酶的方法。
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公开(公告)号：US20090081635A1
公开(公告)日：2009-03-26
申请号：US12261004
申请日：2008-10-29
申请人： DONGFANG LIU , Kevin Pojasek , Zachary Shriver , Kristine Holley , Yosuf El-Shabrawi , Ganesh Venkataraman , Ram Sasisekharan
发明人： DONGFANG LIU , Kevin Pojasek , Zachary Shriver , Kristine Holley , Yosuf El-Shabrawi , Ganesh Venkataraman , Ram Sasisekharan
IPC分类号： C12Q1/00
CPC分类号： C12P19/26 , A61K31/726 , A61K38/51 , C08B37/0075 , C12N9/88 , C12Q1/527 , G01N2333/988 , G01N2500/02 , Y10S530/811 , A61K31/00 , A61K2300/00
摘要： The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
摘要翻译： 本发明涉及肝素酶III及其突变体。 根据本发明已经发现具有降低的酶活性的修饰形式的肝素酶III可用于各种目的，包括肝素样糖胺聚糖（HLGAG）的测序，从溶液中除去活性硫酸乙酰肝素，血管生成抑制等 。 本发明在其它方面涉及使用肝素酶III或通过HLGAG的肝素酶III的酶切割产生的产物来治疗癌症并抑制肿瘤细胞生长和/或转移的方法。
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公开(公告)号：US07399604B2
公开(公告)日：2008-07-15
申请号：US11183323
申请日：2005-07-15
申请人： Ram Sasisekharan , Ganesh Venkataraman , Zachary Shriver , Dongfang Liu , Mallikarjun Sundaram , Yiwei Qi
发明人： Ram Sasisekharan , Ganesh Venkataraman , Zachary Shriver , Dongfang Liu , Mallikarjun Sundaram , Yiwei Qi
IPC分类号： C12Q1/56
CPC分类号： G01N33/66 , A61K31/727 , C08B37/0075 , C12P19/26 , G01N33/86 , G01N2400/10 , G01N2400/40 , Y10T436/143333
摘要： The invention relates to methods and products for characterizing and using polysaccharides. Low molecular weight heparin products and methods of use are described. Methods for characterizing purity and activity of polysaccharide preparations including glycosaminoglycans such as heparin are also described.
摘要翻译： 本发明涉及用于表征和使用多糖的方法和产品。 描述了低分子量肝素产品和使用方法。 还描述了表征包括糖胺聚糖如肝素的多糖制剂的纯度和活性的方法。
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公开(公告)号：US07396824B2
公开(公告)日：2008-07-08
申请号：US11433224
申请日：2006-05-11
CPC分类号： C12N9/16 , A61K38/00 , C12P19/04 , G01N33/5041 , G01N2400/40
摘要： The invention relates to 2-O sulfatase and uses thereof. In particular, the invention relates to recombinantly produced 2-O sulfatase, functional variants and nucleic acid molecules that encode these molecules. The invention also provides methods of using 2-O sulfatase for a variety of purposes, including degrading and analyzing glycosaminoglycans (GAGs) present in a sample. For instance, 2-O sulfatase may be used for determining the purity, identity, composition and sequence of glycosaminoglycans present in a sample. The invention also relates to methods of inhibiting angiogenesis and cellular proliferation as well as methods for treating cancer, neurodegenerative disease, atherosclerosis and microbial infection using 2-O sulfatase and/or GAG fragments produced by degradation with 2-O sulfatase.
摘要翻译： 本发明涉及2-O硫酸酯酶及其用途。 特别地，本发明涉及编码这些分子的重组产生的2-O硫酸酯酶，功能变体和核酸分子。 本发明还提供了用于各种目的的2-O硫酸酯酶的方法，包括降解和分析样品中存在的糖胺聚糖（GAG）。 例如，2-O硫酸酯酶可用于测定样品中存在的糖胺聚糖的纯度，同一性，组成和顺序。 本发明还涉及抑制血管生成和细胞增殖的方法以及使用2-O硫酸酯酶降解产生的2- O硫酸酯酶和/或GAG片段来治疗癌症，神经变性疾病，动脉粥样硬化和微生物感染的方法。
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40.发明授权Methods for preparing low molecular weight heparin with modified heparinase III 有权用改良的肝素酶III制备低分子肝素的方法公开(公告)号：US07390633B2
公开(公告)日：2008-06-24
申请号：US11406214
申请日：2006-04-18
申请人： Dongfang Liu , Kevin Pojasek , Zachary Shriver , Kristine Holley , Yosuf El-Shabrawi , Ganesh Venkataraman , Ram Sasisekharan
发明人： Dongfang Liu , Kevin Pojasek , Zachary Shriver , Kristine Holley , Yosuf El-Shabrawi , Ganesh Venkataraman , Ram Sasisekharan
CPC分类号： C12P19/26 , A61K31/726 , A61K38/51 , C08B37/0075 , C12N9/88 , C12Q1/527 , G01N2333/988 , G01N2500/02 , Y10S530/811 , A61K31/00 , A61K2300/00
摘要： The invention relates to heparinase III and mutants thereof. Modified forms of heparinase III having reduced enzymatic activity which are useful for a variety of purposes, including sequencing of heparin-like glycosaminoglycans (HLGAGs), removing active heparan sulfate from a solution, inhibition of angiogenesis, etc. have been discovered according to the invention. The invention in other aspects relates to methods of treating cancer and inhibiting tumor cell growth and/or metastasis using heparinase III, or products produced by enzymatic cleavage by heparinase III of HLGAGs.
摘要翻译： 本发明涉及肝素酶III及其突变体。 根据本发明已经发现具有降低的酶活性的修饰形式的肝素酶III可用于各种目的，包括肝素样糖胺聚糖（HLGAG）的测序，从溶液中除去活性硫酸乙酰肝素，血管生成抑制等 。 本发明在其它方面涉及使用肝素酶III或通过HLGAG的肝素酶III的酶切割产生的产物来治疗癌症并抑制肿瘤细胞生长和/或转移的方法。
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