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公开(公告)号：US08530193B2
公开(公告)日：2013-09-10
申请号：US13264905
申请日：2010-04-30
申请人： Aaron Kelley , Chuanbin Liu , Colin Mitchinson
发明人： Aaron Kelley , Chuanbin Liu , Colin Mitchinson
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12P19/02 , C12P19/12 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091
摘要： This present disclosure relates to methods for improved production of proteins from a cell culture, particularly to culture components and conditions that can preferentially increase the expression of proteins produced from genes under the control of xylanase gene promoter sequences. The improved methods can be used for the production of enzyme compositions with enhanced xylanase and hemicellulolytic activity.
摘要翻译： 本公开涉及用于从细胞培养物，特别是可以优先增加由木聚糖酶基因启动子序列控制下的基因产生的蛋白质表达的培养物组分和条件改进蛋白质生产的方法。 改进的方法可用于生产具有增强的木聚糖酶和半纤维素分解活性的酶组合物。
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公开(公告)号：US08236546B2
公开(公告)日：2012-08-07
申请号：US13092086
申请日：2011-04-21
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
摘要翻译： 披露的是灰腐质霉Cel7A（CBH1.1），H.jecorina CBH1变体或嗜热链球菌CBH1的变体，编码它们的核酸及其生产方法。 变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代。
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公开(公告)号：US08232080B2
公开(公告)日：2012-07-31
申请号：US13107702
申请日：2011-05-13
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译： 本文描述的是H.jecorina CBH I，Cel7酶的变体。 本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。
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公开(公告)号：US08097445B2
公开(公告)日：2012-01-17
申请号：US11088306
申请日：2005-03-23
CPC分类号： C12N9/2437 , C07K2319/00 , C07K2319/50 , C12N15/625 , C12N15/80 , C12P21/02 , C12P21/06 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.
摘要翻译： 本发明涉及异源外切纤维素酶融合构建体，其编码具有纤维素分解活性的融合蛋白，其包含衍生自真菌外切纤维二糖水解酶的催化结构域和源自内切葡聚糖酶的催化结构域。 本发明还涉及包含异源外切纤维素酶融合构建体的载体和真菌宿主细胞以及生产纤维素酶融合蛋白和酶纤维素酶组合物的方法。
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公开(公告)号：US20110229956A1
公开(公告)日：2011-09-22
申请号：US13107702
申请日：2011-05-13
申请人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
发明人： Anthony Day , Frits Goedegebuur , Peter Gualfetti , Colin Mitchinson , Paulien Neefe , Mats Sandgren , Andrew Shaw , Jerry Stahlberg
CPC分类号： C12N9/2437 , C11D3/38645 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , D21C5/005 , Y02E50/16
摘要： Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
摘要翻译： 本文描述的是H.jecorina CBH I，Cel7酶的变体。 本发明提供了具有改善的热稳定性和可逆性的新型纤维二糖水解酶。
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公开(公告)号：US20100087353A1
公开(公告)日：2010-04-08
申请号：US12360720
申请日：2009-01-27
申请人： Peter Gualfetti , Colin Mitchinson , Jay Phillips
发明人： Peter Gualfetti , Colin Mitchinson , Jay Phillips
CPC分类号： D06P5/158 , C11D3/38645 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P1/228 , D06P5/02 , D06P5/137
摘要： The present invention relates to novel variant EGIII or EGIII-like cellulases that have improved stability. The variant cellulases have performance sensitive residues replaced to a residue having modified stability.
摘要翻译： 本发明涉及具有改善的稳定性的新型EGIII或EGIII样纤维素酶。 变性纤维素酶具有性能敏感残基被替换为具有修饰稳定性的残基。
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公开(公告)号：US07094588B2
公开(公告)日：2006-08-22
申请号：US10441625
申请日：2003-05-19
申请人： Peter Gualfetti , Colin Mitchinson , Jay Phillips
发明人： Peter Gualfetti , Colin Mitchinson , Jay Phillips
CPC分类号： D06P5/158 , C11D3/38645 , C12N9/2437 , C12Y302/01004 , D06M16/003 , D06P1/228 , D06P5/02 , D06P5/137
摘要： The present invention relates to novel variant EGIII or EGIII-like cellulases that have improved stability. The variant cellulases have performance sensitive residues replaced to a residue having modified stability.
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公开(公告)号：US20060057672A1
公开(公告)日：2006-03-16
申请号：US11088306
申请日：2005-03-23
申请人： Benjamin Bower , Edmund Larenas , Colin Mitchinson
发明人： Benjamin Bower , Edmund Larenas , Colin Mitchinson
CPC分类号： C12N9/2437 , C07K2319/00 , C07K2319/50 , C12N15/625 , C12N15/80 , C12P21/02 , C12P21/06 , C12Y302/01004 , C12Y302/01091
摘要： The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions.
摘要翻译： 本发明涉及异源外切纤维素酶融合构建体，其编码具有纤维素分解活性的融合蛋白，其包含衍生自真菌外切纤维二糖水解酶的催化结构域和源自内切葡聚糖酶的催化结构域。 本发明还涉及包含异源外切纤维素酶融合构建体的载体和真菌宿主细胞以及生产纤维素酶融合蛋白和酶纤维素酶组合物的方法。
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公开(公告)号：US20050037459A1
公开(公告)日：2005-02-17
申请号：US10810277
申请日：2004-03-26
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01091
摘要： Disclosed are variants of Humicola grisea Cel7A (CBH1.1), H. jecorina CBH1 variant or S. thermophilium CBH1, nucleic acids encoding the same and methods for producing the same. The variant cellulases have the amino acid sequence of a glycosyl hydrolase of family 7A wherein one or more amino acid residues are substituted.
摘要翻译： 披露的是灰腐质霉Cel7A（CBH1.1），H.jecorina CBH1变体或嗜热链球菌CBH1的变体，编码它们的核酸及其生产方法。 变体纤维素酶具有7A族糖基水解酶的氨基酸序列，其中一个或多个氨基酸残基被取代。
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40.发明授权Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same 有权突变体EGIII纤维素酶，编码这种EGIII组合物的DNA及其获得方法公开(公告)号：US06407046B1
公开(公告)日：2002-06-18
申请号：US09633084
申请日：2000-08-04
申请人： Timothy Fowler , Colin Mitchinson
发明人： Timothy Fowler , Colin Mitchinson
IPC分类号： C11D3386
CPC分类号： C12N9/2437 , C12Y302/01004
摘要： The present invention relates to variant EGIII cellulases that have improved stability and/or performance. The variant cellulases have replacements at sensitive residues to improve stability and/or performance.
摘要翻译： 本发明涉及具有改善的稳定性和/或性能的变体EGIII纤维素酶。 变体纤维素酶在敏感残基中具有替代物以改善稳定性和/或性能。
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