公开(公告)号：US20210155976A1

公开(公告)日：2021-05-27

申请号：US17126439

申请日：2020-12-18

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Frank Bergmann ,  Dieter Heindl ,  Michael Schraeml ,  Johannes Stoeckel

IPC: C12Q1/6816

Abstract: The present report relates to hybridizing single-stranded (ss-) oligonucleotides which entirely consist of locked nucleic acid (LNA) monomers. The present document shows hybridization experiments with pairs of entirely complementary ss-oligonucleotides which fail to form a duplex within a given time interval. The present report provides methods to identify such incompatible oligonucleotide pairs. In another aspect, the present report provides pairs of complementary ss-oligonucleotides which are capable of rapid duplex formation. The present report also provides methods to identify and select compatible oligonucleotide pairs. In yet another aspect the present report provides use of compatible oligonucleotide pairs as binding partners in binding assays, e.g. immunoassays.

公开(公告)号：US20200277406A1

公开(公告)日：2020-09-03

申请号：US16821537

申请日：2020-03-17

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Andreas Gallusser ,  Dieter Heindl ,  Michael Schraeml ,  Christoph Seidel ,  Herbert von der Eltz

IPC: C07K19/00 ,  C07K16/32 ,  C07K16/18 ,  C07K16/28

Abstract: A binding agent of the Formula A-a′:a-S-b:b′-B:X(n), wherein A as well as B is a monovalent binder, a′:a as well as b:b′ is a binding pair wherein a′ and a do not interfere with the binding of b to b′ and vice versa, S is a spacer of at least 1 nm in length, :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a′, a, b, b′ or S, (n) is an integer and at least 1, — represents a covalent bond, and the linker a-S-b has a length of 6 to 100 nm. Also disclosed are methods of producing such binding agent and certain uses thereof.

公开(公告)号：US20190324025A1

公开(公告)日：2019-10-24

申请号：US16450181

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Lars Hillringhaus ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Florian Huber ,  Hans-Peter Josel ,  Thomas Meier ,  Michael Schraeml ,  Edgar Voss

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20160194410A1

公开(公告)日：2016-07-07

申请号：US15079897

申请日：2016-03-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Andreas Gallusser ,  Dieter Heindl ,  Michael Schraeml ,  Christoph Seidel ,  Herbert von der Eltz

IPC: C07K19/00

CPC classification number: C07K19/00 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/626 ,  C07K2317/92 ,  C07K2317/94

Abstract: A binding agent of the Formula A-a′:a-S-b:b′-B:X(n), wherein A as well as B is a monovalent binder, a′:a as well as b:b′ is a binding pair wherein a′ and a do not interfere with the binding of b to b′ and vice versa, S is a spacer of at least 1 nm in length, :X denotes a functional moiety bound either covalently or via a binding pair to at least one of a′, a, b, b′ or S, (n) is an integer and at least 1, — represents a covalent bond, and the linker a-S-b has a length of 6 to 100 nm. Also disclosed are methods of producing such binding agent and certain uses thereof.

Abstract translation: 式Aa'的结合剂：aSb：b'-B：X（n），其中A和B是一价结合剂，a：a以及b：b'是一个结合对，其中a' 并且a不干扰b与b'的结合，反之亦然，S是长度至少为1nm的间隔物：X表示共价或通过结合对结合至至少一个“ ，a，b，b'或S，（n）为整数且至少为1， - 表示共价键，连接体aSb的长度为6〜100nm。 还公开了生产这种结合剂及其某些用途的方法。

公开(公告)号：US20130288267A1

公开(公告)日：2013-10-31

申请号：US13923646

申请日：2013-06-21

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Dieter Heindl ,  Alfred Mertens ,  Christoph Rutz ,  Michael Schraeml ,  Monika Soukupova ,  Claudio Sustmann ,  Michael Tacke ,  Jan van Dieck

IPC: C07K16/46

CPC classification number: C07K16/468 ,  C07K16/18 ,  C07K16/2863 ,  C07K16/32 ,  C07K16/44 ,  C07K2317/31 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/92 ,  C07K2317/94 ,  G01N33/6857

Abstract: A bivalent binding agent, capable of binding a polypeptide dimer, consisting of two monovalent binders linked to each other via a linker, the first monovalent binder binds an epitope of a first target polypeptide comprised in said dimer and the second monovalent binder binds to an epitope of a second target polypeptide comprised in said dimer. Each monovalent binder has a Kdiss in the range of 5×10−3/sec to 10−4/sec, and the bivalent binding agent has a Kdiss of 3×10−5/sec or less. Methods of making and using such bivalent binding agent in histological staining procedures are also disclosed.

Abstract translation: 能够结合多肽二聚体的二价结合剂，其由通过接头彼此连接的两个单价结合物组成，所述第一单价键合物结合包含在所述二聚体中的第一目标多肽的表位，并且所述第二单价键合物结合表位 的包含在所述二聚体中的第二目标多肽。 每个一价粘合剂的Kdiss在5×10-3 /秒至10-4 /秒的范围内，二价结合剂的Kdiss为3×10-5 /秒或更小。 还公开了在组织学染色方法中制备和使用这种二价结合剂的方法。