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公开(公告)号：US6071392A
公开(公告)日：2000-06-06
申请号：US89350
申请日：1998-06-03
CPC分类号： C12Q1/005 , Y10S435/817
摘要： A cholesterol sensor is disclosed which comprises an electrode system having a measuring electrode and a counter electrode formed on an electrically insulating base plate, an electrode coating layer for covering the electrode system and a reaction reagent layer formed on or in the vicinity of the electrode coating layer, wherein the reaction reagent layer comprises at least an enzyme for catalyzing cholesterol oxidation, an enzyme having a cholesterol ester hydrolyzing activity and a surfactant, the electrode coating layer comprises at least one selected from the group consisting of water-soluble cellulose derivatives and saccharides and is contained at such a concentration that imparts sufficient viscosity to a sample solution for enabling it to hinder invasion of said surfactant into said electrode system when said electrode coating layer is dissolved in said sample solution supplied to said sensor. This configuration best eliminates impairment of sensor response due to electrode degeneration caused by invading surfactant into the electrode system.
摘要翻译： 公开了一种胆固醇传感器，其包括具有测量电极和形成在电绝缘基板上的对电极的电极系统，用于覆盖电极系统的电极涂层和形成在电极涂层上或附近的反应试剂层 其中所述反应试剂层至少包含用于催化胆固醇氧化的酶，具有胆固醇酯水解活性的酶和表面活性剂，所述电极涂层包含选自水溶性纤维素衍生物和糖类中的至少一种 并且以使得当样品溶液溶解在提供给所述传感器的所述样品溶液中时能使其阻止所述表面活性剂侵入所述电极系统的浓度给予足够的粘度。 该配置最好地消除由于将表面活性剂侵入电极系统引起的电极变性的传感器响应的损害。
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公开(公告)号：USRE36991E
公开(公告)日：2000-12-19
申请号：US375705
申请日：1999-08-13
申请人： Tomohiro Yamamoto , Mariko Miyashita , Toshihiko Yoshioka , Satoko Tsuji (nee Fujisawa) , Shiro Nankai
发明人： Tomohiro Yamamoto , Mariko Miyashita , Toshihiko Yoshioka , Satoko Tsuji (nee Fujisawa) , Shiro Nankai
CPC分类号： C12Q1/002 , C12Q1/001 , Y10S435/817
摘要： A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.
摘要翻译： 用于以高精度快速定量包含在各种生物样品中的特定成分的生物传感器具有电绝缘基底，包括形成在绝缘基底的一个面上的工作电极和对电极的电极系统，以及形成在绝缘基底上的反应层 绝缘基座与电极系统紧密接触。 反应层至少含有亲水性聚合物，缓冲液和与缓冲液分离的酶。
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公开(公告)号：US6117289A
公开(公告)日：2000-09-12
申请号：US986743
申请日：1997-12-08
CPC分类号： C12Q1/005
摘要： A cholesterol sensor facilitating rapid measurement of serum cholesterol levels is disclosed. The sensor comprises an electrode system composed of at least a measuring electrode and a counter electrode and disposed on an electrically insulating base plate and a reaction layer formed on or in the vicinity of the electrode system. The reaction layer contains cholesterol esterase for catalyzing the conversion of cholesterol ester into cholesterol, cholesterol oxidase and a surfactant.
摘要翻译： 公开了促进血清胆固醇水平快速测量的胆固醇传感器。 传感器包括至少由测量电极和对电极组成的电极系统，并设置在电绝缘基板上，反应层形成在电极系统上或附近。 反应层含有用于催化胆固醇酯转化为胆固醇，胆固醇氧化酶和表面活性剂的胆固醇酯酶。
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公开(公告)号：US6059946A
公开(公告)日：2000-05-09
申请号：US58800
申请日：1998-04-13
申请人： Keiko Yukawa , Toshihiko Yoshioka , Shiro Nankai
发明人： Keiko Yukawa , Toshihiko Yoshioka , Shiro Nankai
CPC分类号： C12Q1/001 , C12Q1/004 , Y10S435/817
摘要： The present invention provides a stable and cost-effective biosensor. The biosensor includes an electrically insulating base plate, an electrode system that includes at least a working electrode and counter electrode formed on the base plate, a reaction layer containing at least an enzyme, an electron acceptor that is formed on, or in the vicinity of, the electrode system, and a divalent water-soluble metallic salt provided in, or in the vicinity of, the reaction layer.
摘要翻译： 本发明提供了一种稳定且具有成本效益的生物传感器。 生物传感器包括电绝缘基板，电极系统，其至少包括形成在基板上的工作电极和对电极，至少含有酶的反应层，形成在其上或附近的电子受体 ，电极系统和设置在反应层中或其附近的二价水溶性金属盐。
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35.发明授权Biosensor and a method for measuring a concentration of a substrate in a sample 失效生物传感器和测量样品中底物浓度的方法
公开(公告)号：US5264103A
公开(公告)日：1993-11-23
申请号：US961528
申请日：1992-10-15
申请人： Toshihiko Yoshioka , Shiro Nankai
发明人： Toshihiko Yoshioka , Shiro Nankai
CPC分类号： C12Q1/004 , G01N2333/904 , Y10S435/817
摘要： The present invention provides a biosensor comprising an electrical insulating substrate, a main electrode system formed on the substrate and having a working electrode and a counter electrode, a reaction layer provided in contact with or in the vicinity of the main electrode system and containing an oxidoreductase, and a sub electrode system as a reference provided with an interval from the main electrode system and having a working electrode and a counter electrode.
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公开(公告)号：US06383819B1
公开(公告)日：2002-05-07
申请号：US09492979
申请日：2000-01-27
IPC分类号： G01N33543
CPC分类号： G01N27/3272 , G01N33/92 , G01N2800/044 , G01N2800/52 , Y10S435/803 , Y10S435/814 , Y10S435/817 , Y10S435/962 , Y10S435/973 , Y10S436/806 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in step (b) from a value obtained in step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
摘要翻译： 本公开涉及一种用于测定低密度脂蛋白胆固醇的方法，包括以下步骤：（a）测量含有至少高密度脂蛋白，低密度脂蛋白，极低密度脂蛋白和乳糜微粒的样品中的总胆固醇水平，和（b） 测量样品中高密度脂蛋白，极低密度脂蛋白和乳糜微粒中的胆固醇水平，其中通过从步骤（a）中获得的值减去步骤（b）中获得的值来确定低密度脂蛋白中的胆固醇水平。 本发明能够同时测定低密度脂蛋白和总胆固醇水平的胆固醇水平，便于一次获取两种生物信息。
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公开(公告)号：US5958199A
公开(公告)日：1999-09-28
申请号：US815760
申请日：1997-03-12
IPC分类号： G01N27/327 , C12Q1/00 , G01N27/26
CPC分类号： C12Q1/001 , Y10S435/817
摘要： Disclosed is a high performance biosensor free from the effect of any components existing in a sample solution other than a substrate. The biosensor comprises an electrically insulating base plate, an electrode system including a working electrode and a counter electrode provided on the base plate, and a reaction layer containing a hydrophilic polymer, an enzyme and an electron acceptor located over the electrode system. The reaction layer contains an excess amount of hydrophilic polymer in a range of 150 to 1,000% by weight of the enzyme.
摘要翻译： 本发明公开了一种高效能的生物传感器，不受基片以外的样品溶液中存在的任何成分的影响。 生物传感器包括电绝缘基板，包括设置在基板上的工作电极和对电极的电极系统，以及包含位于电极系统上的亲水性聚合物，酶和电子受体的反应层。 反应层含有过量的亲水性聚合物，其量为该酶的150-1000重量％。
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公开(公告)号：US5759445A
公开(公告)日：1998-06-02
申请号：US641765
申请日：1996-05-02
CPC分类号： G01N33/96 , G01N33/92 , G01N2800/044 , Y10S516/928 , Y10T436/104165
摘要： There is disclosed an aqueous dispersed solution as a standard solution for determining lipid levels, having a dispersion form and a particle size, which are similar to those of serum lipids. A lipid-dispersed solution is obtained by evaporating an organic solvent from a mixture prepared by adding cholesterol, a phospholipid, a bile acid or bile acid salt, and a neutral lipid and/or a cholesterol ester in the organic solvent, swelling the resultant thin-film like mixture with water or buffer heated at a temperature higher than a phase transition temperature of the lecithin, and then dispersing the solution by a physical shear force. There can be easily obtained a solution having a cholesterol concentration of about less than 1,000 mg/dl, which is extremely stable and can be stored for a long period of time.
摘要翻译： 公开了水分散溶液作为用于测定脂质水平的标准溶液，具有与血清脂质相似的分散体形式和粒径。 通过将有机溶剂从通过在有机溶剂中加入胆固醇，磷脂，胆汁酸或胆汁酸盐和中性脂质和/或胆固醇酯制备的混合物蒸发而获得脂质分散溶液，将所得薄片 在与高于卵磷脂相变温度的温度下加热的水或缓冲液混合，然后通过物理剪切力分散溶液。 可以容易地获得胆固醇浓度大约小于1,000mg / dl的溶液，其极为稳定并且可以长期储存。
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公开(公告)号：US06762062B2
公开(公告)日：2004-07-13
申请号：US09961930
申请日：2001-09-24
IPC分类号： G01N3353
CPC分类号： G01N27/3272 , G01N33/92 , G01N2800/044 , G01N2800/52 , Y10S435/803 , Y10S435/814 , Y10S435/817 , Y10S435/962 , Y10S435/973 , Y10S436/806 , Y10S436/815 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： The present disclosure relates to a method for determining cholesterol in low density lipoprotein comprising the steps of (a) measuring total cholesterol level in a sample containing at least high density lipoprotein, low density lipoprotein, very low density lipoprotein and chylomicron, and (b) measuring cholesterol levels in the high density lipoprotein, very low density lipoprotein and chylomicron in the sample, wherein the cholesterol level in the low density lipoprotein is determined by subtracting a value obtained in the step (b) from a value obtained in the step (a). The present invention enables concurrent determination of cholesterol level in low density lipoprotein and total cholesterol level, facilitating acquisition of two types of biological information at a time.
摘要翻译： 本公开涉及一种用于测定低密度脂蛋白胆固醇的方法，包括以下步骤：（a）测量含有至少高密度脂蛋白，低密度脂蛋白，极低密度脂蛋白和乳糜微粒的样品中的总胆固醇水平，和（b） 测量样品中高密度脂蛋白，极低密度脂蛋白和乳糜微粒中的胆固醇水平，其中通过从步骤（a）中获得的值减去步骤（b）中获得的值来确定低密度脂蛋白中的胆固醇水平 ）。 本发明能够同时测定低密度脂蛋白和总胆固醇水平的胆固醇水平，便于一次获取两种生物信息。
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公开(公告)号：US06471839B1
公开(公告)日：2002-10-29
申请号：US09572797
申请日：2000-05-17
IPC分类号： G01N27327
摘要： The present invention provides a biosensor which enables quick measurement and is excellent in storage characteristic. This biosensor comprises an electrically insulating base plate on which an electrode system having at least a measuring electrode and a counter electrode is formed, a cover member which is integrated to the base plate so as to form a sample solution supply path for supplying a sample solution to the electrode system between the cover member and the base plate, and a carrier composed of fiber supporting a reagent containing at least an oxidoreductase, and the carrier is placed in the sample solution supply path. Preferably, the carrier is constituted at least of two carrier pieces and each carrier piece supports a different reagent.
摘要翻译： 本发明提供一种生物传感器，其能够快速测量并且具有优异的储存特性。 该生物传感器包括电绝缘基板，其上形成有至少具有测量电极和对电极的电极系统，盖构件，其与基板一体地形成用于提供样品溶液的样品溶液供应路径 涉及覆盖构件和基板之间的电极系统，以及由支撑含有至少一种氧化还原酶的试剂的纤维构成的载体，载体被放置在样品溶液供给路径中。 优选地，载体至少由两个载体片构成，并且每个载体片支撑不同的试剂。
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