Methods for reducing enzyme consumption in second generation bioethanol fermentation in the prescence of lignin
    31.
    发明授权
    Methods for reducing enzyme consumption in second generation bioethanol fermentation in the prescence of lignin 有权
    第二代生物乙醇发酵中木质素测定中消耗酶消耗的方法

    公开(公告)号:US07972826B2

    公开(公告)日:2011-07-05

    申请号:US12863601

    申请日:2009-02-02

    Abstract: Provided are methods of liquefaction, saccharification and fermentation of pre-treated lignocellulosic biomass in production of bioethanol. Addition of PEG or surfactant is effective to enhance cellulase hydrolysis yields at high dry matter (>20%) at surprisingly low concentrations compared with comparable results in low dry matter hydrolysis. This effect is most pronounced at comparatively low cellulase loading

    Abstract translation: 提供在生物乙醇生产中预处理的木质纤维素生物质的液化,糖化和发酵的方法。 与低干物质水解中可比较的结果相比,添加PEG或表面活性剂有助于在高干物质(> 20%)下以令人惊讶的低浓度提高纤维素酶水解产率。 在相对较低的纤维素酶负载量<7 FPU(g DM)-1下,这种作用最显着。 在高干物质含量下,通过加入少量的PEG或表面活性剂可以减少纤维素酶消耗而不损失水解产率。

    Device for discharging pretreated biomass from higher to lower pressure regions

    公开(公告)号:US10087578B2

    公开(公告)日:2018-10-02

    申请号:US14910531

    申请日:2014-08-11

    Applicant: Inbicon A/S

    Abstract: Provided is a discharge device for discharging pretreated biomass from a pressurized reactor. The device comprises: a vessel having an opening to a high pressure region at the top, and configured to be connected with a pressurized biomass pretreatment device; one or more inlet openings situated along the sides of the vessels through which water or liquid may be added; an orifice or valve at a lower part of said vessel, said orifice or valve being configured to eject pretreated biomass, optionally into a pipeline. The discharge device is characterised in that it comprises mechanical agitation means, said agitation means comprising an agitation element arranged in the interior of said vessel at a lower part of said vessel, and being configured to provide agitation of the content of said vessel, wherein said agitation means being adapted to withstand a pressure in the interior of a said vessel of 10 bar or more. The agitation means provides for a temperature equalization within a specific vertical range of heights of an aqueous slurry present in said vessel, thereby eliminating disadvantages of the prior art devices and methods.

    Particle pump methods and devices
    37.
    发明授权

    公开(公告)号:US09211515B2

    公开(公告)日:2015-12-15

    申请号:US13062522

    申请日:2010-08-27

    Abstract: Methods and devices are provided for transfer of particulate material, such as biomass feedstocks, into and out of pressurized reactors. Improved sluice devices have an L-shaped sluice chamber having an upper, vertical component in communication with a horizontal loading chamber and a lower component in communication with a vertical reactor inlet or outlet. Piston valves seal the sluice inlet and outlet by axial displacement across the vertical component of the sluice chamber and across the vertical reactor inlet or outlet. Relative to other methods for reactor unloading, these devices consume less steam and significantly reduce furfural content of unloaded, pretreated biomass. An optional hybrid plug/sluice method of biomass feeding using the devices permits biomass loading at sluice pressures intermediate between atmospheric and reactor pressure, thereby reducing “pump cycle” time and increasing biomass throughput capacity.

    Non-Sterile Fermentation of Bioethanol
    38.
    发明申请
    Non-Sterile Fermentation of Bioethanol 审中-公开
    生物乙醇的无菌发酵

    公开(公告)号:US20150299734A1

    公开(公告)日:2015-10-22

    申请号:US14253890

    申请日:2014-04-16

    Applicant: Inbicon A/S

    Inventor: Jan Larsen

    Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.

    Abstract translation: 存在一定范围的浓度,其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现,例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。

    Non-sterile fermentation of bioethanol
    39.
    发明授权
    Non-sterile fermentation of bioethanol 失效
    生物乙醇的无菌发酵

    公开(公告)号:US08703453B2

    公开(公告)日:2014-04-22

    申请号:US13356664

    申请日:2012-01-24

    Applicant: Jan Larsen

    Inventor: Jan Larsen

    Abstract: A range of concentrations exists in which fermentation inhibitors derived from pretreatment of lignocellulosic feed stocks inhibit growth of lactic acid bacteria without affecting fermentive yeast. By optimizing levels of fermentation inhibitors to fall within this range, yeast fermentations of lignocellulosic biomass can be conducted under non-sterile conditions with ethanol yields comparable to those achieved under sterile conditions. Optimised inhibitor levels can be achieved by controlling the water/biomass ratio of a lignocellulosic biomass during and after pretreatment, for example by washing the fiber fraction of a previously pretreated lignocellulosic biomass with a pre-defined amount of fresh water or recycled process solutions. Crude extracts of liquid fraction or process solutions from pretreatment of lignocellulosic biomass can also provide an effective anti-baterial treatment for first generation starch fermentations.

    Abstract translation: 存在一定范围的浓度,其中衍生自木质纤维素原料的预处理的发酵抑制剂抑制乳酸菌的生长而不影响发酵酵母。 通过优化发酵抑制剂的含量落在此范围内,木质纤维素生物质的酵母发酵可以在非无菌条件下进行,乙醇产量与在无菌条件下达到的相似。 优化的抑制剂水平可以通过在预处理期间和之后控制木质纤维素生物质的水/生物量比来实现,例如通过用预定量的淡水或再循环的处理溶液洗涤先前预处理的木质纤维素生物质的纤维部分。 来自木质纤维素生物质预处理的液体馏分或处理溶液的粗提取物也可为第一代淀粉发酵提供有效的抗物质处理。

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