公开(公告)号：US20150133520A1

公开(公告)日：2015-05-14

申请号：US14401612

申请日：2013-05-21

申请人： University of Massachusetts

发明人： Michael P. Czech ,  Mengxi Wang

IPC分类号： C12N5/077 ,  G01N33/50 ,  A61K45/06 ,  A61K31/713 ,  A61K31/7105

CPC分类号： C12N5/0658 ,  A61K9/0019 ,  A61K9/127 ,  A61K9/51 ,  A61K31/7105 ,  A61K31/7115 ,  A61K31/712 ,  A61K31/7125 ,  A61K31/713 ,  A61K45/06 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/12 ,  C12N2310/14 ,  C12N2501/40 ,  C12N2506/1323 ,  C12Y207/11001 ,  G01N33/5023 ,  G01N33/5061 ,  G01N33/5073 ,  G01N2333/91205 ,  G01N2333/91215 ,  G01N2800/10

摘要： Provided herein are methods of inducing differentiation of a mammalian myoblast into a mammalian myocyte that include contacting a mammalian myoblast with an oligonucleotide that decreases Mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of inducing mammalian myoblasts or myocytes to form a myotube that include contacting two or more mammalian myoblasts or two or more mammalian myocytes with an oligonucleotide that decreases Map4k4 mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of identifying a candidate agent useful for inducing muscle formation, and compositions containing an oligonucleotide that decreases Map4k4 mRNA expression in mammalian myoblast or myocyte and one or more additional muscle therapeutic agents and/or muscle-building neutraceuticals.

摘要翻译： 本文提供诱导哺乳动物成肌细胞分化为哺乳动物肌细胞的方法，其包括使哺乳动物成肌细胞与哺乳动物成肌细胞或肌细胞中降低丝裂原活化蛋白激酶激酶激酶4（Map4k4）mRNA表达的寡核苷酸接触。 还提供了诱导哺乳动物成肌细胞或肌细胞形成肌管的方法，其包括使两种或更多种哺乳动物成肌细胞或两种或更多种哺乳动物肌细胞与哺乳动物成肌细胞或肌细胞中降低Map4k4 mRNA表达的寡核苷酸接触。 还提供了鉴定用于诱导肌肉形成的候选药剂的方法，以及含有降低哺乳动物成肌细胞或肌细胞中的Map4k4mRNA表达的寡核苷酸和一种或多种另外的肌肉治疗剂和/或肌肉建立中性药物的组合物。

公开(公告)号：US08852579B2

公开(公告)日：2014-10-07

申请号：US13394798

申请日：2010-11-17

申请人： Helen M. Blau ,  Kostandin Pajcini ,  Jason Pomerantz

发明人： Helen M. Blau ,  Kostandin Pajcini ,  Jason Pomerantz

IPC分类号： A61K35/12 ,  C12Q1/02 ,  C12N5/00 ,  C12N5/077

CPC分类号： C12N5/0658 ,  A61K31/713 ,  A61K35/34 ,  C12N2501/00 ,  C12N2501/40 ,  C12N2501/405 ,  C12N2506/1323 ,  G01N33/502

摘要： Methods are provided for producing cells within a lineage (lineage restricted cells) from post-mitotic differentiated cells of the same lineage ex vivo and in vivo, and for treating a subject in need of tissue regeneration therapy by employing these lineage-restricted cells. In addition, the production of lineage restricted cells from postmitotic tissues derived from patients with diseases allows for a characterization of pathways that have gone awry in these diseases and for screening of drugs that will ameliorate or correct the defects as a means of novel drug discovery. Also provided are kits for performing these methods.

摘要翻译： 提供了用于从离体和体内相同谱系的有丝分裂后分化细胞的谱系（谱系受限细胞）内产生细胞的方法，并且通过使用这些谱系限制细胞来治疗需要组织再生治疗的受试者。 此外，从疾病患者衍生的血管内组织的谱系限制性细胞的产生允许表征在这些疾病中失败的途径，并筛选将改善或纠正缺陷作为新药物发现的手段。 还提供了用于执行这些方法的套件。

公开(公告)号：US20070250165A1

公开(公告)日：2007-10-25

申请号：US11766166

申请日：2007-06-21

申请人： Cato Laurencin ,  Helen Lu ,  Michelle Kofron ,  Saadiq El-Amin ,  Mohamed Attawia

发明人： Cato Laurencin ,  Helen Lu ,  Michelle Kofron ,  Saadiq El-Amin ,  Mohamed Attawia

IPC分类号： A61F2/28

CPC分类号： A61L27/3847 ,  A61F2002/2817 ,  A61F2002/2835 ,  A61K35/12 ,  A61L27/227 ,  A61L27/3608 ,  A61L27/365 ,  A61L2430/02 ,  C12N5/0068 ,  C12N5/0654 ,  C12N2501/155 ,  C12N2506/1323 ,  C12N2533/40

摘要： Bone grafting materials containing a polymer scaffold loaded with bone morphogenetic proteins and populated with muscle cells induced by the bone morphogenetic proteins to exhibit an osteoblastic phenotype and to synthesize bone tissue are provided. Also provided are methods for using these polymer scaffolds in bone grafting procedures.

摘要翻译： 提供了含有装载有骨形态发生蛋白的聚合物支架和由骨形态发生蛋白诱导的肌肉细胞展示成骨细胞表型并合成骨组织的骨移植材料。 还提供了在骨移植过程中使用这些聚合物支架的方法。