公开(公告)号：US09883669B2

公开(公告)日：2018-02-06

申请号：US14939442

申请日：2015-11-12

申请人： BOURBON CORPORATION

发明人： Akito Matsushima ,  Sakiko Takizawa ,  Tadayuki Yokoyama

IPC分类号： C12N5/00 ,  A01N1/02 ,  C12N5/074

CPC分类号： A01N1/0226 ,  A01N1/0221 ,  C12N5/0696 ,  C12N2500/34 ,  C12N2501/00

摘要： Provided are a maintenance medium for primate pluripotent stem cells, and a method for preserving and a method for controlling proliferation of primate pluripotent stem cells using the medium. The maintenance medium for human pluripotent stem cells according to the present invention includes xylose as a saccharide.

公开(公告)号：US20170306296A1

公开(公告)日：2017-10-26

申请号：US15516107

申请日：2015-10-02

申请人： JICHI MEDICAL UNIVERSITY

发明人： Hitoshi ENDO ,  Yasumitsu NAGAO ,  Yutaka HANAZONO ,  Kaoru TOMINAGA ,  Tsukasa OHMORI

IPC分类号： C12N5/074 ,  C12Q1/02 ,  C12N5/0735 ,  C12N15/09 ,  C12Q1/68 ,  C12N5/00

CPC分类号： C12N5/0696 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2267/02 ,  A01K2267/03 ,  C12N5/00 ,  C12N5/0604 ,  C12N5/0606 ,  C12N15/09 ,  C12N2501/00 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/5073

摘要： The present application relates to a method for reestablishing stem cells capable of forming chimeras, and cells obtained by the method. The method of the present invention is a technique for monocloning stem cells, for example, capable of forming chimeras from a heterogeneous cell population to obtain high-quality stem cells.

公开(公告)号：US20170153224A1

公开(公告)日：2017-06-01

申请号：US15126623

申请日：2015-03-26

申请人： Helmholtz Zentrum München - Deutsches Forschungszentrum Für Gesundheit Und Umwelt (GmbH)

发明人： Heiko Lickert ,  Adriana Migliorini ,  Moritz Gegg ,  Erik Bader

IPC分类号： G01N33/50 ,  C12Q1/68 ,  G01N33/68 ,  C12N5/071

CPC分类号： G01N33/507 ,  A61K38/1709 ,  C12N5/0676 ,  C12N2501/00 ,  C12N2501/415 ,  C12N2501/727 ,  C12Q1/6881 ,  C12Q2600/158 ,  G01N33/56966 ,  G01N33/6893 ,  G01N2800/042

摘要： The present invention relates to the use of the biomarker Flattop (Fltp) for distinguishing mature β cells from immature progenitor β cells. The present invention further relates to a method for distinguishing a mature β cell from an immature progenitor β cell, the method comprising: determining the presence or absence of the biomarker Flattop (Fltp) in a β cell; wherein the presence of Fltp in the cell indicates that the cell is a mature β cell and wherein the absence of Fltp in the cell indicates that the cell is an immature progenitor β cell. Furthermore, the present invention relates to a method of identifying a compound suitable for differentiating immature progenitor β cells into mature β cells as well as to a method of identifying a compound suitable for preventing the de-differentiating of mature β cells.

公开(公告)号：US20160348074A1

公开(公告)日：2016-12-01

申请号：US15166934

申请日：2016-05-27

申请人： Agenovir Corporation

发明人： Stephen R. Quake ,  Jianbin Wang

IPC分类号： C12N5/0789 ,  A61K35/28

CPC分类号： A61K35/28 ,  C12N9/22 ,  C12N2501/00

摘要： The invention relates to methods for generating viral-free cells using nucleases for use in transplantation. The nucleases may be CRISPR/Cas9 complexes with guided RNA to target and inactivate viral genomes within cells. The nucleases degrade or destroy the viruses within the cells prior to transplantation.

摘要翻译： 本发明涉及使用用于移植的核酸酶产生无病毒细胞的方法。 核酸酶可能是CRISPR / Cas9复合物与引导RNA靶向和灭活细胞内的病毒基因组。 移植前核酸酶降解或破坏细胞内的病毒。

公开(公告)号：US20160312189A1

公开(公告)日：2016-10-27

申请号：US15103317

申请日：2014-12-16

申请人： FRESENIUS MEDICAL CARE DEUTSCHLAND GMBH

发明人： Ciro TETTA ,  Giovanni CAMUSSI ,  Sara GIUNTI ,  Victor Manuel NAVARRO TABLEROS

IPC分类号： C12N5/071 ,  A61L27/38

CPC分类号： C12N5/0677 ,  A61L27/3804 ,  A61L2430/00 ,  C12N2501/00 ,  C12N2501/998 ,  C12N2506/14 ,  C12N2533/32

摘要： The invention relates to a method of preparing pancreatic islet-like cell structures characterized by a unique combination of morphological and functional features which make them particularly suitable for use in both clinical and drug screening application, as well as to the pancreatic islet-like cell structures obtained therefrom.

摘要翻译： 本发明涉及一种制备胰岛样细胞结构的方法，其特征在于形态和功能特征的独特组合，使其特别适用于临床和药物筛选应用以及胰岛样细胞结构 由此获得。

公开(公告)号：US09328329B2

公开(公告)日：2016-05-03

申请号：US14139152

申请日：2013-12-23

申请人： MULTIPLE SCLEROSIS RESEARCH CENTER OF NEW YORK

发明人： Saud A. Sadiq ,  Violaine K. Harris

IPC分类号： A61K35/30 ,  C12N5/077 ,  C12N5/079 ,  C12N5/0775 ,  C12N5/0797 ,  C12N5/0793

CPC分类号： C12N5/0622 ,  A61K35/30 ,  C12N5/0619 ,  C12N5/0623 ,  C12N5/0663 ,  C12N2501/00 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2502/08 ,  C12N2506/1353

摘要： Methods are provided for treating and/or reducing the severity of multiple sclerosis in a human, by administering autologous mesenchymal stem cell-derived neural precursors. Also described is an in vitro method for differentiating mesenchymal stem-cell derived neural precursor oligodendroglial and neuronal cell types.

摘要翻译： 通过施用自体间充质干细胞衍生的神经前体，提供了用于治疗和/或降低人类多发性硬化症的严重程度的方法。 还描述了用于分化间充质干细胞衍生的神经前体少突神经胶质细胞和神经元细胞类型的体外方法。

公开(公告)号：US09102914B2

公开(公告)日：2015-08-11

申请号：US13257949

申请日：2011-02-03

申请人： Yansong Gu

发明人： Yansong Gu

IPC分类号： C12N15/85 ,  A61K35/00 ,  C12N5/07 ,  C12N5/0735 ,  C12N11/04 ,  C12N11/08 ,  C12N11/10 ,  C12N5/077

CPC分类号： C12N5/06 ,  C12N5/0606 ,  C12N5/0657 ,  C12N11/04 ,  C12N11/08 ,  C12N11/10 ,  C12N2501/00 ,  C12N2502/025 ,  C12N2506/02 ,  C12N2533/30 ,  C12N2533/90

摘要： This disclosure relates generally to methods, apparatuses, and cellular compositions and cellular products that use a unique trophoblast-containing 3D matrix as a compartmental chamber for growing embryoid bodies that can be induced to differentiate into organ-specific cell types.

摘要翻译： 本公开一般涉及使用独特的含滋养层的3D基质作为生长可诱导分化为器官特异性细胞类型的胚状体的室室的方法，装置和细胞组合物和细胞产品。

公开(公告)号：US09072819B2

公开(公告)日：2015-07-07

申请号：US12439090

申请日：2007-08-31

申请人： Jeanine Anna Alphonse Hendriks ,  Jens Uwe Riesle

发明人： Jeanine Anna Alphonse Hendriks ,  Jens Uwe Riesle

IPC分类号： A61F2/00 ,  A61L27/38 ,  A61L27/36 ,  A61L27/52 ,  C12N5/077

CPC分类号： A61L27/3852 ,  A61L27/3633 ,  A61L27/3817 ,  A61L27/52 ,  A61L2430/06 ,  C12N5/0655 ,  C12N2501/00

摘要： The present invention relates in one aspect to the use of a matrix gel comprising chondrocytes or progenitor cells thereof in a density below that of natural cartilage as a cartilage repair implant wherein said cells exhibit increases production of extracellular matrix material.

摘要翻译： 本发明在一个方面涉及包含软骨细胞或祖细胞的基质凝胶的使用，其密度低于天然软骨的密度，作为软骨修复植入物，其中所述细胞表现出细胞外基质材料的产生增加。

公开(公告)号：US20150118198A1

公开(公告)日：2015-04-30

申请号：US14525903

申请日：2014-10-28

申请人： MULTIPLE SCLEROSIS RESEARCH CENTER OF NEW YORK

发明人： Saud A. SADIQ ,  Violaine K. Harris

IPC分类号： C12N5/079 ,  C12N5/0793 ,  A61K35/30

CPC分类号： C12N5/0622 ,  A61K35/30 ,  C12N5/0619 ,  C12N5/0623 ,  C12N5/0663 ,  C12N2501/00 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2502/08 ,  C12N2506/1353

摘要： Methods are provided for treating and/or reducing the severity of multiple sclerosis in a human, by administering autologous mesenchymal stem cell-derived neural precursors. Also described is an in vitro method for differentiating mesenchymal stem-cell derived neural precursor oligodengroglial and neuronal cell types.

公开(公告)号：US20150093762A1

公开(公告)日：2015-04-02

申请号：US14337092

申请日：2014-07-21

申请人： UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

发明人： HIROSHI KUBOTA ,  LOLA M. REID

IPC分类号： C12N5/071 ,  G01N33/569

CPC分类号： C12N5/0018 ,  C12N5/067 ,  C12N5/0672 ,  C12N2500/00 ,  C12N2500/05 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/00 ,  C12N2501/11 ,  C12N2501/235 ,  C12N2501/33 ,  G01N33/56966

摘要： The present invention relates to precursor cells to hepatic stellate cells, compositions comprising same and methods of isolating same. The surface antigenic profile of the precursors is MHC class Ia negative, ICAM-1+, VCAM-1+, β3-integrin+. In addition to expression of these surface markers, the cells also express the intracellular markers desmin, vimentin, smooth muscle α-actin, nestin, hepatocyte growth factor, stromal derived factor-1α and Hlx homeobox transcriptional factor.

摘要翻译： 本发明涉及肝星状细胞的前体细胞，包含其的组合物及其分离方法。 前体的表面抗原谱是MHC Ia类阴性，ICAM-1 +，VCAM-1 +，＆bgr。3-整联蛋白+。 除了这些表面标志物的表达外，细胞还表达细胞内标志物结蛋白，波形蛋白，平滑肌α-肌动蛋白，巢蛋白，肝细胞生长因子，基质衍生因子-1α和Hlx同源异位转录因子。