公开(公告)号：US11236323B2

公开(公告)日：2022-02-01

申请号：US16258557

申请日：2019-01-26

申请人： Toyota Motor Corporation ,  The University of Akron

发明人： Ping Wang ,  Minjuan Zhang ,  Hongfei Jia ,  Archana H. Trivedi ,  Masahiko Ishii

IPC分类号： C12N11/14 ,  C09D5/14 ,  C09D5/16 ,  C12N9/20 ,  C12N9/96 ,  C12N11/04 ,  C12N11/06 ,  C12N9/54 ,  G06F21/55 ,  G06F21/56 ,  G06F21/62 ,  H04L29/06

摘要： The present disclosure relates to compositions and processes in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a linker moiety bound to both said digestive protein and said substrate. The processes include binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate.

公开(公告)号：US20220025350A1

公开(公告)日：2022-01-27

申请号：US17501013

申请日：2021-10-14

申请人： DANISCO US INC.

发明人： JOSHUA ROY BASLER ,  LUIS G. CASCAO-PEREIRA ,  DAVID A. ESTELL ,  JAMES T. KELLIS, JR.

IPC分类号： C12N9/54 ,  C11D3/386 ,  C12N9/52 ,  A23K20/189 ,  C11D11/00

摘要： The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

公开(公告)号：US11225654B2

公开(公告)日：2022-01-18

申请号：US16900404

申请日：2020-06-12

申请人： Toyota Motor Corporation ,  The University of Akron

发明人： Ping Wang ,  Minjuan Zhang ,  Hongfei Jia ,  Archana H. Trivedi ,  Masahiko Ishii

IPC分类号： C12N11/14 ,  C09D5/14 ,  C09D5/16 ,  C12N9/20 ,  C12N9/96 ,  C12N11/04 ,  C12N11/06 ,  C12N9/54 ,  G06F21/55 ,  G06F21/56 ,  G06F21/62 ,  H04L29/06

摘要： Provided are methods and compositions for self-cleaning that include a lysozyme capable of decomposing a microbe, a substrate applied to a solid surface, and a linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said lysozyme and said substrate and covalently linking said lysozyme to a surface of said substrate optionally by an amide bond.

公开(公告)号：US20210292728A1

公开(公告)日：2021-09-23

申请号：US17216735

申请日：2021-03-30

申请人： DANISCO US INC

发明人： LILIA MARIA BABE ,  VIKTOR YURYEVICH ALEKSEYEV ,  NEELAM S. AMIN ,  JOSHUA ROY BASLER ,  DAVID A. ESTELL ,  VICTORIA HUANG ,  DAVID MARQUEZ ,  JEFFREY WAYNE MUNOS ,  REI OTSUKA ,  GEETHA VEERAMUTHU ,  DAVID EDWARD WILDES ,  MICHELLE JACKSON ,  EUAN JOHN MAGENNIS ,  EVA MARIA PEREZ-PRAT VINUESA ,  PHILIP F. SOUTER ,  DAVID JOHN TARBIT

IPC分类号： C12N9/54 ,  C11D3/386

摘要： Disclosed herein is one or more subtilisin variant useful for cleaning applications and in methods of cleaning. One embodiment is directed to one or more subtilisin variant, including one or more Bacillus sp. subtilisin polypeptide variant, and one or more cleaning composition comprising one or more such variant.

公开(公告)号：US11124808B2

公开(公告)日：2021-09-21

申请号：US15506881

申请日：2015-08-27

申请人： Renescience A/S

发明人： Hanne Risbjerg Soerensen ,  Lisa Rosgaard ,  Henrik B. Nielsen ,  Lone Baekgaard ,  Joanna Wawrzynczyk

IPC分类号： C12P7/10 ,  C12N9/54 ,  C12N9/42 ,  C12N9/26 ,  C12N9/24 ,  C12N9/48 ,  C12N9/88 ,  C12P5/02 ,  C12P7/08 ,  C11D3/386 ,  C12N9/20

摘要： The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.

公开(公告)号：US11091750B2

公开(公告)日：2021-08-17

申请号：US16668635

申请日：2019-10-30

申请人： DANISCO US INC

发明人： Andrew Shaw ,  David A Estell ,  Eugene S Sadlowski ,  Hiroshi Oh ,  Louise Wallace ,  Ronaldus Wilhelmus Joannes Hommes ,  Sang-Kyu Lee

IPC分类号： C12N9/54 ,  C11D3/386 ,  C11D1/02

摘要： The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from Bacillus sp. In some more particularly preferred embodiments, the neutral metalloprotease is obtained from B. amyloliquefaciens. In still further preferred embodiments, the neutral metalloprotease is a variant of the B. amyloliquefaciens neutral metalloprotease. In yet additional embodiments, the neutral metalloprotease is a homolog of the the B. amyloliquefaciens neutral metalloprotease. The present invention finds particular use in applications including, but not limited to cleaning, bleaching and disinfecting.

公开(公告)号：US11015148B2

公开(公告)日：2021-05-25

申请号：US16844110

申请日：2020-04-09

申请人： Reckitt Benckiser Finish B.V.

发明人： Lucia Krubasik ,  Frank Dierkes ,  Steffen Lingler ,  Jörg Pflug

IPC分类号： C11D3/386 ,  C12N9/54 ,  C11D3/33 ,  C11D3/36 ,  C11D3/37 ,  C11D3/39 ,  C11D17/04

摘要： A detergent composition comprising a subtilisin variant having the amino acid sequence set forth in SEQ ID NO: 1, and at least one additional ingredient selected from i) bleaches selected from percarbonates, persulphates and organic peracids, ii) aminocarboxylates, or iii) sulphonated polymers, or iv) organophosphoric acids or salts thereof and mixtures thereof is provided. Also provided is a detergent composition comprising a subtilisin variant having the amino acid sequence set forth in SEQ ID NO: 1, wherein the detergent composition is at least partially enveloped in a water soluble or water dispersible package. The compositions exhibit good performance on proteinaceous stains, even when formulated at alkaline pHs. A method of removing proteinaceous stains from surfaces comprising such stains is also provided.

公开(公告)号：US10975366B2

公开(公告)日：2021-04-13

申请号：US16476878

申请日：2017-10-24

申请人： GUANGDONG VTR BIO-TECH CO., LTD

发明人： Yangyuan Li ,  Jianrong Wang ,  Ling Yang ,  Man Qu ,  Lizhi Chen ,  Jiale Huang

IPC分类号： C12N9/54 ,  C12N9/44

摘要： The present invention relates to the field of genetic engineering, particularly to a method for efficiently expressing pullulanase in Bacillus subtilis and recombinant Bacillus subtilis. said method includes steps of constructing modified Bacillus subtilis strain with deletion of alkaline protease gene and neutral protease gene, constructing expression vector including an optimized combination of promoter and signal peptide and pullulanase gene, and transforming said modified Bacillus subtilis strain with by said expression vector. A series of combinations of promoter and signal peptide are optimized to obtain the combination for efficiently expressing pullulanase, provide an industrial application basis.

公开(公告)号：US10920209B2

公开(公告)日：2021-02-16

申请号：US16789555

申请日：2020-02-13

申请人： NOVOZYMES A/S

发明人： Frank Winther Rasmussen ,  Peter Kamp Hansen ,  Lars Lehman Hylling Christensen

IPC分类号： C12N9/54 ,  C12N9/52 ,  C11D3/386

摘要： The present invention relates to novel subtilase variants exhibiting increased stability and preferably on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.

公开(公告)号：US20210009979A1

公开(公告)日：2021-01-14

申请号：US17040207

申请日：2019-03-21

申请人： NOVOZYMES A/S

发明人： Esben Peter Friis ,  Rolf Thomas Lenhard ,  Lars Lehmann Hylling Christensen ,  Carl Mikael Bauer

IPC分类号： C12N9/54 ,  C11D3/386

摘要： The invention relates to subtilase variants and detergent compositions comprising the variants, as well as methods of producing the variants and methods for stabilizing a subtilase variant.