中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

129 records (took 0.032 seconds)

    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE
    41.
    发明申请
    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE 失效
    编号为ZWA1基因的核苷酸序列

    公开(公告)号：US20060205046A1

    公开(公告)日：2006-09-14

    申请号：US11419529

    申请日：2006-05-22

    Applicant: Bettina Mockel ,  Walter Pfefferle ,  Achim Marx ,  Jorn Kalinowski ,  Brigitte Bathe ,  Alfred Puhler

    Inventor： Bettina Mockel ,  Walter Pfefferle ,  Achim Marx ,  Jorn Kalinowski ,  Brigitte Bathe ,  Alfred Puhler

    IPC: C12P13/08 ,  C12N15/74 ,  C12N1/21

    CPC classification number: C07K14/34 ,  C12N9/00 ,  C12P13/08

    Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

    Abstract translation: 本文公开了一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％相同程度的氨基酸序列c）与a）和 b）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。

    Process for the production of L-amino acids by fermentation using coryneform bacteria with an attenuated mqo gene
    42.
    发明授权
    Process for the production of L-amino acids by fermentation using coryneform bacteria with an attenuated mqo gene 有权
    使用具有减毒mqo基因的棒状细菌通过发酵生产L-氨基酸的方法

    公开(公告)号：US07049106B2

    公开(公告)日：2006-05-23

    申请号：US10118325

    申请日：2002-04-09

    Applicant: Mike Farwick ,  Brigitte Bathe ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    Inventor： Mike Farwick ,  Brigitte Bathe ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC: C12P13/04

    CPC classification number: C12N9/0036 ,  C12P13/08

    Abstract: A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.

    Abstract translation: 其中生产L-氨基酸的方法，其中将其中编码苹果酸醌氧化还原酶的mqo基因的表达减弱的棒状细菌（例如谷氨酸棒杆菌）被发酵以产生所需的氨基酸，并且氨基酸浓缩在 培养基或细胞分离。 任选地，所需氨基酸的生物合成途径中的其它基因被增强，和/或减少氨基酸形成的代谢途径被抑制。

    Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity
    45.
    发明授权
    Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity 失效
    通过使用具有减弱的1-磷酸果糖激酶活性的棒状细菌制备氨基酸的方法

    公开(公告)号：US06921651B2

    公开(公告)日：2005-07-26

    申请号：US10098626

    申请日：2002-03-18

    Applicant: Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

    Inventor： Mike Farwick ,  Brigitte Bathe ,  Jennifer Brehme ,  Klaus Huthmacher

    IPC: C12N1/21 ,  C12N9/12 ,  C12N15/54 ,  C12N15/55 ,  C12P13/04 ,  C12P13/08

    CPC classification number: C12N9/1205 ,  C12P13/04 ,  C12P13/08

    Abstract: The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofructokinase are/is attenuated, enriching the desired L-amino acid in the medium or in the cells of the bacteria, and isolating the L-amino acid. Optionally bacteria are employed in which, in addition, further genes of the biosynthetic pathway of the desired L-amino acid are enhanced, or bacteria are employed in which the metabolic pathways that diminish the formation of the desired L-amino acid are at least partly switched off.

    Abstract translation: 本发明涉及一种制备L-氨基酸的方法。 该方法包括发酵产生所需L-氨基酸的棒状细菌，其中至少编码6-磷酸果糖激酶的基因和/或编码1-磷酸果糖激酶的基因被减毒，从而富集所需的L-氨基酸 培养基或细胞中的细胞，并分离L-氨基酸。 任选地，使用细菌，其中另外还需要增加所需L-氨基酸的生物合成途径的其它基因，或使用细菌，其中减少所需L-氨基酸形成的代谢途径至少部分地 关闭

    Nucleotide sequences coding for the pknB gene
    46.
    发明申请
    Nucleotide sequences coding for the pknB gene 有权
    编码pknB基因的核苷酸序列

    公开(公告)号：US20050130277A1

    公开(公告)日：2005-06-16

    申请号：US10940606

    申请日：2004-09-15

    Applicant: Brigitte Bathe ,  Stephan Hans ,  Mike Farwick ,  Thomas Hermann

    Inventor： Brigitte Bathe ,  Stephan Hans ,  Mike Farwick ,  Thomas Hermann

    IPC: C12N9/12 ,  C12P13/04 ,  C07H21/04 ,  C12N1/21 ,  C12N9/10 ,  C12N9/42 ,  C12N15/74

    CPC classification number: C12N9/1205 ,  C12P13/04 ,  C12Y207/01037

    Abstract: An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.

    Abstract translation: 一种分离的多核苷酸，其含有选自以下的多核苷酸序列：（a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％的多核苷酸，（b） 编码含有与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列的多肽，（c）与（a）或（b）的多核苷酸互补的多核苷酸和（ d）包含（a），（b）或（c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用其中至少pknB基因被扩增的棒状杆菌制备L-氨基酸的发酵方法 以及使用含有本发明序列的多核苷酸作为杂交探针。

Patent Agency Ranking