公开(公告)号：US11015162B1

公开(公告)日：2021-05-25

申请号：US17140057

申请日：2021-01-02

Applicant: Inscripta, Inc.

Inventor： Nathan Bade ,  Jorge Bernate ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/42 ,  C12M1/00 ,  C12N13/00

Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.

公开(公告)号：US10927385B2

公开(公告)日：2021-02-23

申请号：US16904405

申请日：2020-06-17

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US10920189B2

公开(公告)日：2021-02-16

申请号：US16904827

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held ,  Eric Abbate ,  Michael Clay ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US10907178B2

公开(公告)日：2021-02-02

申请号：US16426310

申请日：2019-05-30

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader ,  Kevin Ness

IPC: C12M3/00 ,  C12M1/00 ,  B01L3/00 ,  C12N15/87 ,  C12M1/42 ,  C12N15/70 ,  C12N15/81 ,  C12M1/36

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments comprising one or more flow-through electroporation devices or modules are provided to automate genome editing in live cells.

公开(公告)号：US10858761B2

公开(公告)日：2020-12-08

申请号：US16798338

申请日：2020-02-22

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N15/81 ,  C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US10822621B2

公开(公告)日：2020-11-03

申请号：US16548208

申请日：2019-08-22

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader

IPC: C12M3/00 ,  C12M1/00 ,  C12N15/87 ,  C12M1/42 ,  C12N15/70 ,  C12N15/81 ,  C12M1/36

Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.

公开(公告)号：US20200338561A1

公开(公告)日：2020-10-29

申请号：US16928061

申请日：2020-07-14

Applicant: Inscripta, Inc.

Inventor： Jorge Bernate ,  Don Masquelier ,  Phillip Belgrader ,  Brian Van Hatten ,  Bruce Chabansky

IPC: B01L3/00 ,  C12M1/36 ,  C12M1/12 ,  C12M3/00 ,  C12N13/00 ,  B01L7/00 ,  B01L7/02 ,  C12M1/02 ,  C12M1/42 ,  C12M1/00

Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment. The reagent cartridges may include an electroporation device, as well as sample receptacles, reagent receptacles, waste receptacles and the like, and a script for controlling a processor to dispense samples and reagents contained in the receptacles, and to porate cells in the electroporation device. Also described are kits including the cartridges, automated multi-module cell processing instruments including the reagent cartridges and methods of using the reagent cartridges.

公开(公告)号：US10801008B1

公开(公告)日：2020-10-13

申请号：US16930269

申请日：2020-07-15

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87 ,  B01D71/02 ,  B01D63/08 ,  B01D69/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US10723995B1

公开(公告)日：2020-07-28

申请号：US16820292

申请日：2020-03-16

Applicant: Inscripta, Inc.

Inventor： Phillip Belgrader ,  Don Masquelier ,  Andrew Garst ,  Richard Fox

IPC: C12M1/12 ,  B01D25/00 ,  B01D63/08 ,  C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00 ,  B01D69/06 ,  B01D71/02

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：US20200216795A1

公开(公告)日：2020-07-09

申请号：US16823269

申请日：2020-03-18

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Richard Fox ,  Phillip Belgrader ,  Don Masquelier

IPC: C12M1/00 ,  C12M1/26 ,  C12N15/87 ,  C12M3/00

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.