公开(公告)号：US5104795A

公开(公告)日：1992-04-14

申请号：US480003

申请日：1990-02-14

申请人： Jar-How Lee ,  Lindley Blair ,  Arnold Horwitz ,  Raju K. Koduri

发明人： Jar-How Lee ,  Lindley Blair ,  Arnold Horwitz ,  Raju K. Koduri

IPC分类号： C07K14/43 ,  C12N15/62 ,  C12N15/69 ,  C12N15/81

CPC分类号： C07K14/43 ,  C12N15/625 ,  C12N15/69 ,  C12N15/81 ,  C07K2319/02 ,  C07K2319/036

摘要： A shortened PGK promoter ranging from 165 to 404 base pairs of the Saccharomyces PGK promoter is taught. The promoter is operably linked to heterologous genes and improves the expression thereof relative to the full length PGK promoter.

摘要翻译： 教导了来自酿酒酵母PGK启动子的165至404碱基对的缩短的PGK启动子。 启动子可操作地连接到异源基因并相对于全长PGK启动子改进其表达。

公开(公告)号：US20110229462A1

公开(公告)日：2011-09-22

申请号：US13051481

申请日：2011-03-18

申请人： William M. Winston, JR. ,  S. Kirk Wright ,  May Han ,  Lyne Breault ,  Jie Lin ,  Bijan Etemad-Gilbertson ,  Christine Knuehl ,  Jeno Gyuris ,  Arnold Horwitz

发明人： William M. Winston, JR. ,  S. Kirk Wright ,  May Han ,  Lyne Breault ,  Jie Lin ,  Bijan Etemad-Gilbertson ,  Christine Knuehl ,  Jeno Gyuris ,  Arnold Horwitz

IPC分类号： A61K39/395 ,  A61P35/00 ,  C12N5/09

CPC分类号： C07K16/22 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

摘要： The present invention provides a family of binding proteins that bind and neutralize the activity of hepatocyte growth factor (HGF), in particular human HGF. The binding proteins can be used as diagnostic and/or therapeutic agents. With regard to their therapeutic activity, the binding proteins can be used to treat certain HGF responsive disorders, for example, certain HGF responsive tumors.

公开(公告)号：US07582742B2

公开(公告)日：2009-09-01

申请号：US12218914

申请日：2008-07-18

申请人： Linda Masat ,  Mary Haak-Frendscho ,  Gang Chen ,  Arnold Horwitz ,  Marina Roell

发明人： Linda Masat ,  Mary Haak-Frendscho ,  Gang Chen ,  Arnold Horwitz ,  Marina Roell

IPC分类号： C12N5/10 ,  C12N15/13 ,  C12N15/63

CPC分类号： C07K16/244 ,  A61K2039/505 ,  C07K16/245 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2317/92 ,  Y10S514/885

摘要： Methods of treating or preventing an IL-1 related disease or condition in a mammal comprising administering an effective amount of an IL-1β binding antibody or IL-1β binding fragment thereof. An IL-1β binding antibody or IL-1β binding fragment thereof is provided comprising the amino acid sequence of SEQ ID NO:28, and related nucleic acids, vectors, cells, and compositions, and a method of preparing an affinity matured IL-1β binding polypeptide is provided. IL-1β binding antibodies or IL-1β binding fragments thereof are provided which have desirable affinity and potency.

摘要翻译： 治疗或预防哺乳动物中IL-1相关疾病或病症的方法，包括给予有效量的IL-1β结合抗体或其IL-1β结合片段。 提供了包含SEQ ID NO：28的氨基酸序列及相关核酸，载体，细胞和组合物的IL-1β结合抗体或IL-1β结合片段，以及制备亲和力成熟的IL-1β 提供了结合多肽。 提供具有期望的亲和力和效力的IL-1β结合抗体或其IL-1β结合片段。

公开(公告)号：US07531166B2

公开(公告)日：2009-05-12

申请号：US11472813

申请日：2006-06-21

申请人： Linda Masat ,  Mary Haak-Frendscho ,  Gang Chen ,  Arnold Horwitz ,  Marina Roell

发明人： Linda Masat ,  Mary Haak-Frendscho ,  Gang Chen ,  Arnold Horwitz ,  Marina Roell

IPC分类号： C07K16/24 ,  A61K39/395

CPC分类号： C07K16/244 ,  A61K2039/505 ,  C07K16/245 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76 ,  C07K2317/92 ,  Y10S514/885

摘要： An IL-1β binding antibody or IL-1β binding fragment thereof comprising the amino acid sequence of SEQ ID NO: 2, and related nucleic acids, vectors, cells, and compositions, as well as method of using same to treat or prevent a disease, and a method of preparing an affinity matured IL-1β binding polypeptide. IL-1β binding antibodies or IL-1β binding fragments thereof are provided which have desirable affinity and potency.

摘要翻译： 包含SEQ ID NO：2的氨基酸序列的IL-1β结合抗体或IL-1β结合片段，以及相关的核酸，载体，细胞和组合物，以及其用于治疗或预防疾病的方法 以及制备亲和力成熟的IL-1β结合多肽的方法。 提供具有期望的亲和力和效力的IL-1β结合抗体或其IL-1β结合片段。

公开(公告)号：US20080145894A1

公开(公告)日：2008-06-19

申请号：US11831691

申请日：2007-07-31

申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

IPC分类号： C12P21/04

CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

摘要： Recombinant expression vectors are provided comprising a 3′UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。

公开(公告)号：US20070281334A1

公开(公告)日：2007-12-06

申请号：US11673539

申请日：2007-02-09

申请人： Arnold Horwitz

发明人： Arnold Horwitz

IPC分类号： C07K14/47 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N15/65 ,  C12N15/66 ,  C12N2800/107 ,  C12N2830/00 ,  C12P21/02

摘要： The present invention provides novel methods and materials for increasing the expression of recombinant polypeptides. Methods and materials of the invention allow increased expression of transcription units that include recombinant DNA sequences which encode polypeptides of interest. The present invention provides expression vectors which contain multiple copies of a transcription unit encoding a polypeptide of interest separated by at least one selective marker gene and methods for sequentially transforming or transfecting host cells with expression vectors to increase transcription unit dosage and expression.

摘要翻译： 本发明提供了用于增加重组多肽表达的新方法和材料。 本发明的方法和材料允许增加包括编码感兴趣多肽的重组DNA序列的转录单位的表达。 本发明提供表达载体，其含有编码由至少一种选择标记基因分离的感兴趣多肽的转录单位的多个拷贝，以及用表达载体依次转化或转染宿主细胞以增加转录单位剂量和表达的方法。

公开(公告)号：US20060121604A1

公开(公告)日：2006-06-08

申请号：US11295006

申请日：2005-12-05

申请人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

发明人： Masahisa Handa ,  Arnold Horwitz ,  Eddie Bautista ,  Robyn Cotter

IPC分类号： C12Q1/68 ,  C12N15/63 ,  C12N15/74 ,  C12N15/00 ,  C12N15/70 ,  C12N15/09

CPC分类号： C07K16/00 ,  C12N7/00 ,  C12N15/85 ,  C12N2710/16221 ,  C12N2710/16243 ,  C12N2800/108 ,  C12N2820/10 ,  C12N2820/55 ,  C12N2820/60 ,  C12N2830/42

摘要： Recombinant expression vectors are provided comprising a 3′ UTR of a light chain and an Epstein-Barr virus origin of replication. Also provided are host cells comprising such vectors and methods of producing recombinant protein with such vectors. Additional methods of producing a recombinant protein involve contacting cells with a first and second vector, each of which encode a different polypeptide chain, and wherein the second vector is present in an amount which is about 1.5 to 2.5 times as much as that of the first vector. Cells also can be transfected with a recombinant transient expression vector encoding a protein and are cultured in a medium in a membrane-enhanced culturing vessel to produce recombinant protein.

摘要翻译： 提供重组表达载体，其包含轻链的3'UTR和Epstein-Barr病毒复制起点。 还提供了包含这种载体的宿主细胞和用这种载体产生重组蛋白的方法。 生产重组蛋白质的其它方法包括使细胞与第一和第二载体接触，第一和第二载体各自编码不同的多肽链，其中第二载体的存在量为第一载体的约1.5至2.5倍 向量。 细胞也可以用编码蛋白质的重组瞬时表达载体转染，并在膜增强培养容器中的培养基中培养以产生重组蛋白。