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64 条记录 (耗时 0.033 秒)

    Method for producing polymer layers
    42.
    发明授权
    Method for producing polymer layers 有权
    生产聚合物层的方法

    公开(公告)号：US08846132B2

    公开(公告)日：2014-09-30

    申请号：US10514758

    申请日：2003-05-16

    申请人： Carina Horn ,  Joachim Hoenes ,  Wolfgang-Reinhold Knappe

    发明人： Carina Horn ,  Joachim Hoenes ,  Wolfgang-Reinhold Knappe

    IPC分类号： C12Q1/00 ,  C08F2/48 ,  G01N33/543 ,  C12Q1/32 ,  C12Q1/26

    CPC分类号： C12Q1/32 ,  C12Q1/002 ,  C12Q1/26 ,  G01N33/54353 ,  G01N33/54373 ,  Y02P20/582

    摘要： A method for producing a polymer layer on a transparent support. A photopolymerizable liquid composition is applied to the support. The photopolymerizable liquid composition is irradiated through the support such that polymerization of the liquid composition starts at the surface of the support and ends at a predetermined distance over the surface of the support. Only partial polymerization of the liquid composition takes place. A polymer layer with a predetermined thickness is formed on the support wherein the predetermined thickness is less than the thickness of the photopolymerizable liquid composition. The remaining photopolymerizable liquid composition is removed from the entire polymer layer.

    摘要翻译： 一种在透明支撑体上制备聚合物层的方法。 将可光聚合的液体组合物施加到载体上。 通过支撑体照射可光聚合的液体组合物，使得液体组合物的聚合在支撑体的表面开始，并在支撑体的表面上以预定距离终止。 仅发生液体组合物的部分聚合。 在预定厚度小于可光聚合液体组合物的厚度的支撑体上形成具有预定厚度的聚合物层。 剩余的可光聚合液体组合物从整个聚合物层中除去。

    Fluorescence spectroscopy in absorbing media
    43.
    发明授权
    Fluorescence spectroscopy in absorbing media 有权
    吸收介质中的荧光光谱

    公开(公告)号：US08501113B2

    公开(公告)日：2013-08-06

    申请号：US12942356

    申请日：2010-11-09

    申请人： Wolfgang Petrich ,  Claudia Gaessler-Dietsche ,  Carina Horn ,  Kai Hebestreit ,  Jean-Michel Asfour ,  Klemens Bardelang ,  Gerrit Kocherscheidt

    发明人： Wolfgang Petrich ,  Claudia Gaessler-Dietsche ,  Carina Horn ,  Kai Hebestreit ,  Jean-Michel Asfour ,  Klemens Bardelang ,  Gerrit Kocherscheidt

    IPC分类号： G01N33/533

    CPC分类号： C12Q1/008 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/54 ,  G01N33/52 ,  G01N33/66 ,  Y10T436/144444

    摘要： The invention relates to processes and devices for detecting an analyte in a sample by fluorescence measurement of a fluorophore, wherein the detection medium which contains a fluorophore or a precursor of the fluorophore is admixed with an absorber whose absorbance spectrum superimposes the fluorescence excitation range of the fluorophore. The system consisting of the fluorophore and the absorber, which is produced in the detection medium, has an altered effective fluorescence excitation range with an altered fluorescence excitation maximum. Illumination with fluorescence excitation light can take place within the range of this altered excitation maximum. The measured signal obtained from determining fluorescence emission exhibits only low dependence on the wavelength of the excitation light.

    摘要翻译： 本发明涉及通过荧光团的荧光测定来检测样品中分析物的方法和装置，其中将含有荧光团或荧光团前体的检测介质与吸收光谱与吸收光谱的荧光激发范围相加 荧光团。 由荧光团和在检测介质中产生的吸收体组成的系统具有改变的有效荧光激发范围，具有改变的荧光激发最大值。 荧光激发光的照明可以在这种改变的激发最大值的范围内进行。 从确定荧光发射获得的测量信号仅显示对激发光的波长的低依赖性。

    Amine-N-oxide redox indicators for fluorimetric determination of analytes
    44.
    发明授权
    Amine-N-oxide redox indicators for fluorimetric determination of analytes 有权
    用于荧光测定分析物的胺-N-氧化物氧化还原指标

    公开(公告)号：US08431410B2

    公开(公告)日：2013-04-30

    申请号：US12757372

    申请日：2010-04-09

    申请人： Carina Horn ,  Joachim Hoenes ,  Jürgen Spinke

    发明人： Carina Horn ,  Joachim Hoenes ,  Jürgen Spinke

    IPC分类号： G01N21/76

    CPC分类号： G01N21/6428 ,  G01N2021/7786 ,  Y10T436/104998 ,  Y10T436/106664 ,  Y10T436/144444

    摘要： A reagent for detecting an analyte by a redox reaction and a fluorimetric determination is disclosed. The reagent comprises a compound of formula (I): wherein R1 and R2 are each independently selected from R, (CH2CH2O)mR, COR, COOR, and OCOR; each R3 is independently selected from NO2, CN, R, OR, OCOR, COOR, SR, and halogen; R is H or C1-C4 alkyl, where alkyl is optionally substituted with one or more functional group independently selected from the group consisting of halogen, OR, SR, NR2, COOR, CONR2, SO3R and salts thereof, and PO(OR)3 and salts thereof; m is an integer from 1 to 20; and n is 1, 2, or 3. The reagent may further comprise an enzyme or a coenzyme for reducing or oxidizing an analyte.

    摘要翻译： 公开了一种用于通过氧化还原反应和荧光测定来检测分析物的试剂。 试剂包含式（I）化合物：其中R 1和R 2各自独立地选自R，（CH 2 CH 2 O）m R，COR，COOR和OCOR; 每个R 3独立地选自NO 2，CN，R，OR，OCOR，COOR，SR和卤素; R是H或C 1 -C 4烷基，其中烷基任选被一个或多个独立地选自卤素，OR，SR，NR 2，COOR，CONR 2，SO 3 R及其盐和PO（OR）3 及其盐; m为1〜20的整数; 并且n为1,2或3.试剂可以进一步包含用于还原或氧化分析物的酶或辅酶。