公开(公告)号：US11274286B2

公开(公告)日：2022-03-15

申请号：US16460118

申请日：2019-07-02

Applicant: Codexis, Inc.

Inventor： Da Duan ,  Oscar Alvizo ,  Jovana Nazor ,  Harvinder Chagger Maniar ,  James Nicholas Riggins ,  Jonathan Vroom ,  Santhosh Sivaramakrishnan ,  Hao Yang ,  Anna Fryszkowska ,  Mark A. Huffman ,  Joshua N. Kolev ,  Iman Farasat ,  Agustina Rodriguez-Granillo ,  Deeptak Verma

IPC: C12N9/88 ,  C12P21/02 ,  C12N15/63

Abstract: The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.

公开(公告)号：US11162105B2

公开(公告)日：2021-11-02

申请号：US16460025

申请日：2019-07-02

Applicant: Codexis, Inc.

Inventor： Scott J. Novick ,  Nikki Dellas ,  Vesna Mitchell ,  Da Duan ,  Jovana Nazor ,  Oscar Alvizo ,  Auric Anthony Sowell-Kantz ,  Jeffrey C. Moore ,  Mark Huffman ,  Agustina Rodriguez-Granillo ,  Deeptak Verma ,  Nicholas M. Marshall ,  Jay Russell ,  Keith A. Canada

IPC: C12N15/63

Abstract: The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US11021729B2

公开(公告)日：2021-06-01

申请号：US16605576

申请日：2018-04-13

Applicant: Codexis, Inc.

Inventor： Jeffrey C. Moore ,  Jack Liang ,  Jonathan Penfield ,  Jovana Nazor ,  Nikki Dellas ,  Vesna Mitchell ,  Da Duan ,  Iman Farasat ,  Agustina Rodriguez-Granillo ,  Grant Murphy ,  Nicholas Marshall

IPC: C12P41/00 ,  C12N9/02 ,  C12N9/04 ,  C12N15/70

Abstract: The present invention provides engineered ketoreductase and phosphite dehydrogenase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase and phosphite dehydrogenase enzymes, as well as polynucleotides encoding the engineered ketoreductase and phosphite dehydrogenase enzymes, host cells capable of expressing the engineered ketoreductase and phosphite dehydrogenase enzymes, and methods of using the engineered ketoreductase and phosphite dehydrogenase enzymes to synthesize a chiral catalyst used in the synthesis of antiviral compounds, such as nucleoside inhibitors. The present invention further provides methods of using the engineered enzymes to deracemize a chiral alcohol in a one-pot, multi-enzyme system.

公开(公告)号：US20180187180A1

公开(公告)日：2018-07-05

申请号：US15861849

申请日：2018-01-04

Applicant: Codexis, Inc.

Inventor： Oscar Alvizo ,  David Elgart ,  Robert Kevin Orr ,  James Nicholas Riggins ,  Anna Fryszkowska ,  Katrina W. Lexa ,  Xiang Yi ,  Da Duan ,  Courtney Dianne Moffett ,  Nikki Dellas ,  Vesna Mitchell

IPC: C12N9/84 ,  C12P21/02 ,  C07K14/62

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.