公开(公告)号：US12110513B2

公开(公告)日：2024-10-08

申请号：US17120646

申请日：2020-12-14

申请人： Codexis, Inc.

发明人： Margie Tabuga Borra-Garske ,  Oscar Alvizo ,  Lillian Jasmine Miller ,  Jesse B. Slaton ,  Aksiniya Lyubenova Petkova ,  Nandhitha Subramanian ,  Joshua Kolev ,  Anna Fryszkowska ,  Agustina Rodriguez-Granillo ,  Grant Murphy

IPC分类号： C12N9/12

CPC分类号： C12N9/1205 ,  C12Y207/01033

摘要： The present invention provides engineered pantothenate kinase (PanK) enzymes, polypeptides having PanK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PanK enzymes are also provided. The present invention further provides compositions comprising the PanK enzymes and methods of using the engineered PanK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US12084697B2

公开(公告)日：2024-09-10

申请号：US18158339

申请日：2023-01-23

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  David Elgart ,  Robert Kevin Orr ,  James Nicholas Riggins ,  Anna Fryszkowska ,  Katrina W. Lexa ,  Xiang Yi ,  Da Duan ,  Courtney Dianne Moffett ,  Nikki Dellas ,  Vesna Mitchell

IPC分类号： C12N15/09 ,  C07K14/62 ,  C12N1/00 ,  C12N9/84 ,  C12P21/02

CPC分类号： C12N9/84 ,  C07K14/62 ,  C12P21/02 ,  C12Y305/01011 ,  C07K2319/21

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：US11965192B2

公开(公告)日：2024-04-23

申请号：US17537941

申请日：2021-11-30

申请人： Codexis, Inc.

发明人： Jovana Nazor ,  Vesna Mitchell ,  David Elgart ,  Katrina W. Lexa ,  Nikki Dellas ,  Robert Kevin Orr ,  Oscar Alvizo ,  Ravi David Garcia ,  Judy Victoria Antonio Viduya ,  Courtney Dianne Moffett

IPC分类号： C12N9/84 ,  C07K14/62 ,  C12P21/02

CPC分类号： C12N9/84 ,  C07K14/62 ,  C12P21/02 ,  C12Y305/01011

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

公开(公告)号：US20230295599A1

公开(公告)日：2023-09-21

申请号：US18154475

申请日：2023-01-13

申请人： Codexis, Inc.

发明人： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC分类号： C12N9/88 ,  C12N15/00

CPC分类号： C12N9/88 ,  C12Y403/01024 ,  C12N15/00 ,  A61K48/00

摘要： The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US11618911B2

公开(公告)日：2023-04-04

申请号：US17830650

申请日：2022-06-02

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12N9/06 ,  C12P13/02 ,  C12P13/00 ,  C12P17/18 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/16

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US11591588B2

公开(公告)日：2023-02-28

申请号：US17091479

申请日：2020-11-06

申请人： Codexis, Inc.

发明人： Oscar Alvizo ,  David Elgart ,  Robert Kevin Orr ,  James Nicholas Riggins ,  Anna Fryszkowska ,  Katrina W. Lexa ,  Xiang Yi ,  Da Duan ,  Courtney Dianne Moffett ,  Nikki Dellas ,  Vesna Mitchell

IPC分类号： A61K38/00 ,  A61K38/28 ,  C12N9/84 ,  C07K14/62 ,  C12P21/02

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：US20220325256A1

公开(公告)日：2022-10-13

申请号：US17711451

申请日：2022-04-01

申请人： Codexis, Inc.

发明人： Margie Tabuga Borra-Garske ,  Oscar Alvizo ,  Lillian Jasmine Miller ,  Aksiniya Lyubenova Petkova

IPC分类号： C12N9/12

摘要： The present invention provides engineered acetate kinase (AcK) enzymes, polypeptides having AcK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing AcK enzymes are also provided. The present invention further provides compositions comprising the AcK enzymes and methods of using the engineered AcK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.

公开(公告)号：US11466259B2

公开(公告)日：2022-10-11

申请号：US16460376

申请日：2019-07-02

申请人： Codexis, Inc.

发明人： Christopher Michael Micklitsch ,  Oscar Alvizo ,  Jovana Nazor ,  Harvinder Chagger Maniar ,  Mikayla Jianghongxia Krawczyk ,  Margie Tabuga Borra-Garske ,  Nandhitha Subramanian ,  Anna Fryszkowska ,  Nicholas M. Marshall ,  Agustina Rodriguez-Granillo ,  Deeptak Verma ,  Dewan Andrews

IPC分类号： C12N9/04 ,  C12N15/52 ,  C12P21/02 ,  C12N9/02

摘要： The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

公开(公告)号：US20220315961A1

公开(公告)日：2022-10-06

申请号：US17830650

申请日：2022-06-02

申请人： CODEXIS, INC.

发明人： Haibin Chen ,  Steven J. Collier ,  Jovana Nazor ,  Joly Sukumaran ,  Derek Smith ,  Jeffrey C. Moore ,  Gregory Hughes ,  Jacob Janey ,  Gjalt W. Huisman ,  Scott J. Novick ,  Nicholas J. Agard ,  Oscar Alvizo ,  Gregory A. Cope ,  Wan Lin Yeo ,  Stefanie Ng Minor

IPC分类号： C12P13/02 ,  C12P13/00 ,  C12P17/18 ,  C12P13/04 ,  C12P13/06 ,  C12P17/10 ,  C12P17/12 ,  C12P17/16 ,  C12N9/06

摘要： The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

公开(公告)号：US20220290110A1

公开(公告)日：2022-09-15

申请号：US17635163

申请日：2020-09-03

申请人： Codexis, Inc.

发明人： Da Duan ,  Auric Anthony Sowell-Kantz ,  Aksiniya Lyubenova Petkova ,  Jovana Nazor ,  Nandhitha Subramanian ,  Oscar Alvizo

IPC分类号： C12N9/08

摘要： The present invention provides engineered peroxidase enzymes, polypeptides having peroxidase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing peroxidase enzymes are also provided. The present invention further provides compositions comprising the peroxidase enzymes and methods of using the engineered peroxidase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.