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41.发明授权Broad-spectrum δ-endotoxins and method and kit for detecting same and for detecting polynucleotides encoding same 有权广谱δ-内毒素及其检测方法和试剂盒，并用于检测编码该多核苷酸的多核苷酸公开(公告)号：US07927598B2
公开(公告)日：2011-04-19
申请号：US12276689
申请日：2008-11-24
申请人： Thomas Malvar , Amy Jelen Gilmer
发明人： Thomas Malvar , Amy Jelen Gilmer
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S977/902
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的对鞘翅目，二ter和鳞翅目昆虫的杀虫活性的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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42.发明申请Plants transformed with polynucleotides encoding broad-spectrum delta-endotoxins 有权用编码广谱δ-内毒素的多核苷酸转化的植物公开(公告)号：US20060174372A1
公开(公告)日：2006-08-03
申请号：US11372065
申请日：2006-04-10
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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公开(公告)号：US06521442B2
公开(公告)日：2003-02-18
申请号：US09916956
申请日：2001-07-27
申请人： Thomas Malvar , Amy Jelen Gilmer
发明人： Thomas Malvar , Amy Jelen Gilmer
IPC分类号： C12N120
CPC分类号： C12N15/8286 , C07K14/325 , C07K2319/00 , Y02A40/162 , Y10S435/975 , Y10S977/914 , Y10S977/924 , Y10S977/925
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
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公开(公告)号：US06281016B1
公开(公告)日：2001-08-28
申请号：US09253331
申请日：1999-02-19
申请人： Thomas Malvar , Amy Jelen Gilmer
发明人： Thomas Malvar , Amy Jelen Gilmer
IPC分类号： C12N504
CPC分类号： C12N15/8286 , C07K14/325 , C07K2319/00 , Y02A40/162 , Y10S435/975 , Y10S977/914 , Y10S977/924 , Y10S977/925
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and tansgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的对鞘翅目，二ter和鳞翅目昆虫的杀虫活性的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的变态植物也是本发明的方面。
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45.发明授权Secreted insecticidal protein and gene compositions from Bacillus thuringiensis and uses therefor 有权来自苏云金芽孢杆菌的分泌的杀虫蛋白和基因组成，并用于此公开(公告)号：US08569583B2
公开(公告)日：2013-10-29
申请号：US13171519
申请日：2011-06-29
申请人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
发明人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C07K14/325
CPC分类号： C07K14/32 , C07K14/325 , C12N15/8286
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。
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公开(公告)号：US07455981B2
公开(公告)日：2008-11-25
申请号：US11880607
申请日：2007-07-23
申请人： Thomas Malvar , Amy Jelen Gilmer
发明人： Thomas Malvar , Amy Jelen Gilmer
IPC分类号： G01N33/53 , G01N33/554
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S977/902
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的对鞘翅目，二ter和鳞翅目昆虫的杀虫活性的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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公开(公告)号：US20080182279A1
公开(公告)日：2008-07-31
申请号：US11880607
申请日：2007-07-23
申请人： Thomas Malvar , Amy Jelen Gilmer
发明人： Thomas Malvar , Amy Jelen Gilmer
IPC分类号： G01N33/569
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162 , Y10S977/902
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
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48.发明授权Plants transformed with polynucleotides encoding broad-spectrum delta-endotoxins 有权用编码广谱δ-内毒素的多核苷酸转化的植物公开(公告)号：US07304206B2
公开(公告)日：2007-12-04
申请号：US11372065
申请日：2006-04-10
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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49.发明申请Insect inhibitory Bacillus thuringiensis proteins, fusions, and methods of use therefor 有权昆虫抑制性苏云金芽孢杆菌蛋白，融合物及其使用方法公开(公告)号：US20070208168A1
公开(公告)日：2007-09-06
申请号：US11796742
申请日：2007-04-27
CPC分类号： A01N37/46 , A01N63/02 , C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Novel insect inhibitory proteins are disclosed comprising two different components, both of which are required for biological activity. Various methods of linking both components together, so that a single protein provides insect inhibitory activity, are disclosed. Also disclosed are novel Bacillus thuringiensis nucleic acid sequences encoding Coleopteran-inhibitory crystal proteins, designated tIC100 (29-kDa) and tlC101 (14-kDa). Also disclosed are methods of making and using nucleic acid sequences in the development of the transgenic plant cells containing the novel nucleic acid sequences disclosed herein.
摘要翻译： 公开了新的昆虫抑制蛋白，其包含两种不同的组分，这两种组分都是生物活性所必需的。 公开了将两种组分连接在一起的各种方法，使得单一蛋白提供昆虫抑制活性。 还公开了称为tIC100（29-kDa）和tlC101（14-kDa）的编码鞘翅目抑制性晶体蛋白的新型苏云金芽孢杆菌核酸序列。 还公开了在含有本文公开的新型核酸序列的转基因植物细胞的开发中制备和使用核酸序列的方法。
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公开(公告)号：US07070982B2
公开(公告)日：2006-07-04
申请号：US10739482
申请日：2003-12-18
CPC分类号： C07K14/325 , C07K2319/00 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity and broader insect host range against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
摘要翻译： 公开了具有改进的杀虫活性和更广泛的昆虫宿主范围的新型合成修饰的苏云金芽孢杆菌嵌合晶体蛋白，其针对鞘翅目，双翅目和鳞翅目昆虫。 还公开了编码这些新肽的核酸片段。 公开了制备和使用这些基因和蛋白质的方法以及重组表达和合适宿主细胞转化的方法。 转化的宿主细胞和表达修饰的内毒素的转基因植物也是本发明的方面。
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