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    Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor
    1.
    发明申请
    Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor 审中-公开
    苏云金芽孢杆菌的分泌杀虫蛋白和基因组成及其用途

    公开(公告)号：US20080040827A1

    公开(公告)日：2008-02-14

    申请号：US10581763

    申请日：2004-12-14

    申请人： Judith Donovan ,  William Donovan ,  James Engleman ,  Thomas Malvar ,  John Pitkin

    发明人： Judith Donovan ,  William Donovan ,  James Engleman ,  Thomas Malvar ,  John Pitkin

    IPC分类号： A01H5/00 ,  A01H1/00 ,  A61K38/00 ,  C07H21/04 ,  C07K14/325 ,  C12N15/00 ,  C12N15/82 ,  C12Q1/68

    CPC分类号： C07K14/32 ,  C07K14/325 ,  C12N15/8286

    摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

    摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

    Secreted insecticidal protein and gene compositions from Bacillus thuringiensis and uses therefor
    2.
    发明授权
    Secreted insecticidal protein and gene compositions from Bacillus thuringiensis and uses therefor 有权
    来自苏云金芽孢杆菌的分泌的杀虫蛋白和基因组成，并用于此

    公开(公告)号：US08569583B2

    公开(公告)日：2013-10-29

    申请号：US13171519

    申请日：2011-06-29

    申请人： Judith Donovan ,  William Donovan ,  James Engleman ,  Thomas Malvar ,  John Pitkin

    发明人： Judith Donovan ,  William Donovan ,  James Engleman ,  Thomas Malvar ,  John Pitkin

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32 ,  C07K14/325

    CPC分类号： C07K14/32 ,  C07K14/325 ,  C12N15/8286

    摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

    摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

    Insecticidal proteins secreted from bacillus thuringiensis and uses therefor
    3.
    发明申请
    Insecticidal proteins secreted from bacillus thuringiensis and uses therefor 有权
    从苏云金芽孢杆菌分泌的杀虫蛋白，用于此

    公开(公告)号：US20060191034A1

    公开(公告)日：2006-08-24

    申请号：US10563270

    申请日：2004-07-06

    申请人： James Baum ,  Judith Donovan ,  William Donovan ,  James Engleman ,  Karina Krasomil-Osterfeld ,  John Pitkin ,  James Roberts

    发明人： James Baum ,  Judith Donovan ,  William Donovan ,  James Engleman ,  Karina Krasomil-Osterfeld ,  John Pitkin ,  James Roberts

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N15/82 ,  C07K14/325 ,  A01N63/00

    CPC分类号： A01N63/02 ,  C07K14/32

    摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

    摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。 公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

    IN PLANTA RNAi CONTROL OF FUNGI
    9.
    发明申请
    IN PLANTA RNAi CONTROL OF FUNGI 审中-公开
    真菌的PLANTA RNAi控制

    公开(公告)号：US20080022423A1

    公开(公告)日：2008-01-24

    申请号：US11670409

    申请日：2007-02-01

    申请人： James Roberts ,  John Pitkin ,  Thomas Adams

    发明人： James Roberts ,  John Pitkin ,  Thomas Adams

    IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N1/20 ,  C12N5/04 ,  C12N5/06 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12N15/8282

    摘要： The present invention relates to control of fungal and oomycete plant pathogens by inhibiting one or more biological functions. The invention provides methods and compositions for such control. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pathogen, a reduction in disease may be obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic agents for use in protecting plants from pathogen infection.

    摘要翻译： 本发明涉及通过抑制一种或多种生物学功能来控制真菌和卵菌纲植物病原体。 本发明提供了用于这种控制的方法和组合物。 通过将本发明提供的一种或多种重组双链RNA分子送入病原体，可以通过抑制基因表达来获得疾病的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受病原体感染的转基因试剂的特定组合的方法。