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1.发明授权Secreted insecticidal protein and gene compositions from Bacillus thuringiensis and uses therefor 有权来自苏云金芽孢杆菌的分泌的杀虫蛋白和基因组成，并用于此公开(公告)号：US08569583B2
公开(公告)日：2013-10-29
申请号：US13171519
申请日：2011-06-29
申请人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
发明人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32 , C07K14/325
CPC分类号： C07K14/32 , C07K14/325 , C12N15/8286
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。
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2.发明申请Secreted Insecticidal Protein and Gene Compositions from Bacillus Thuringiensis and Uses Therefor 审中-公开苏云金芽孢杆菌的分泌杀虫蛋白和基因组成及其用途公开(公告)号：US20080040827A1
公开(公告)日：2008-02-14
申请号：US10581763
申请日：2004-12-14
申请人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
发明人： Judith Donovan , William Donovan , James Engleman , Thomas Malvar , John Pitkin
CPC分类号： C07K14/32 , C07K14/325 , C12N15/8286
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。
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3.发明申请公开(公告)号：US20060191034A1
公开(公告)日：2006-08-24
申请号：US10563270
申请日：2004-07-06
申请人： James Baum , Judith Donovan , William Donovan , James Engleman , Karina Krasomil-Osterfeld , John Pitkin , James Roberts
发明人： James Baum , Judith Donovan , William Donovan , James Engleman , Karina Krasomil-Osterfeld , John Pitkin , James Roberts
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C07K14/325 , A01N63/00
摘要： The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
摘要翻译： 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离出蛋白质，并显示对包括科罗拉多马铃薯甲虫（Lymantria dispar）和南方玉米根虫（Diabrotica undecempunctata）在内的鞘翅目昆虫的杀虫活性。 公开了与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 还公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。
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4.发明申请Methods for detecting Bacillus thuringiensis cryET33 and cry ET34 polypeptides 有权检测苏云金芽孢杆菌cryET33和哭泣ET34多肽的方法公开(公告)号：US20060051822A1
公开(公告)日：2006-03-09
申请号：US11231104
申请日：2005-09-20
申请人： William Donovan , Judith Donovan , Annette Slaney
发明人： William Donovan , Judith Donovan , Annette Slaney
IPC分类号： A61K39/395 , G01N33/554 , C07K16/12
CPC分类号： G01N33/56911 , C07K14/325 , G01N2333/325 , Y10S435/81 , Y10S530/825 , Y10T436/143333
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译： 公开了包含对鞘翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株，包括红粉甲虫幼虫（Tribolium castaneum）和日本甲虫幼虫（Popillia japonica）。 还公开了新颖的苏云金芽孢杆菌晶体毒素基因，命名为cryET33和cryET34，其编码烯醇毒力晶体蛋白CryET33（29-kDa）晶体蛋白，cryET34基因编码14-kDa CryET34晶体蛋白。 CryET33和CryET34晶体蛋白对红粉甲虫幼虫和日本甲虫幼虫有毒。 还公开了制备和使用包含本发明的新核酸序列的转基因细胞的方法。
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公开(公告)号：US20060253925A1
公开(公告)日：2006-11-09
申请号：US11485621
申请日：2006-07-12
申请人： Mark Rupar , William Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette Slaney , Thomas Malvar , James Baum
发明人： Mark Rupar , William Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette Slaney , Thomas Malvar , James Baum
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。
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公开(公告)号：US20060260008A1
公开(公告)日：2006-11-16
申请号：US11485079
申请日：2006-07-12
申请人： Mark Rupar , William Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette Slaney , Thomas Malvar , James Baum
发明人： Mark Rupar , William Donovan , Chih-Rei Chu , Elizabeth Pease , Yuping Tan , Annette Slaney , Thomas Malvar , James Baum
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Disclosed are novel insecticidal polypeptides, and compositions comprising these polypeptides, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that contain nucleic acid segments that encode the disclosed δ-endotoxin polypeptides. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising these polynucleotide sequences, as well as methods for controlling an insect population, such as Colorado potato beetle, southern corn rootworm and western corn rootworm, and for conferring to a plant resistance to a target insect species.
摘要翻译： 公开了新型杀虫多肽，以及包含这些多肽，其肽片段和特异性抗体的组合物。 还公开了载体，转化的宿主细胞和含有编码公开的δ-内毒素多肽的核酸片段的转基因植物。 还公开了鉴定相关多肽和多核苷酸的方法，制备和使用包含这些多核苷酸序列的转基因细胞的方法，以及用于控制昆虫种群的方法，例如科罗拉多马铃薯甲虫，南方玉米根虫和西方玉米根虫，并赋予 对植物对目标昆虫物种的抗性。
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7.发明申请Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and Ctenocephalides SPP. 失效苏云金芽孢杆菌CryET29组合物对鞘翅目昆虫和Ctenocephalides SPP有毒。公开(公告)号：US20070163000A1
公开(公告)日：2007-07-12
申请号：US11712704
申请日：2007-03-01
申请人： Mark Rupar , William Donovan , Yuping Tan , Annette Slaney
发明人： Mark Rupar , William Donovan , Yuping Tan , Annette Slaney
CPC分类号： A01N63/00 , C07K14/325 , C12N15/8286 , G01N2430/10 , Y02A40/162 , Y10S530/825
摘要： Disclosed is a novel δ-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.
摘要翻译： 公开了一种名为CryET29的新型三角内含物，其表现出对iphonapteran昆虫具有杀虫活性，包括猫跳蚤幼虫（Ctenocephalides felis）以及鞘翅目昆虫，包括南方玉米根虫（Diabrotica undecimpunctata），西方玉米根虫 （D. virgifera），科罗拉多马铃薯甲虫（Leptinotarsa decemlineata），日本甲虫（Popillia japonica）和红粉甲虫（Tribolium castaneum））。 还公开了编码CryET29，重组载体，宿主细胞和包含cryET29 DNA片段的转基因植物的核酸片段。 公开了制备和使用所公开的蛋白质和核酸片段的方法以及用于在体内和体外检测cryET29和CryET29序列的测定和诊断试剂盒。
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8.发明申请Lepidopteran-active bacillus thuringiensis delta-endotoxin polynucleotides, compositions, and methods of use 有权鳞翅目激活型苏云金芽孢杆菌δ-内毒素多核苷酸，组合物和使用方法公开(公告)号：US20070061919A1
公开(公告)日：2007-03-15
申请号：US11487921
申请日：2006-07-17
申请人： James Baum , Chih-Rei Chu , William Donovan , Amy Gilmer , Mark Rupar
发明人： James Baum , Chih-Rei Chu , William Donovan , Amy Gilmer , Mark Rupar
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against lepidopteran insects. Also disclosed are novel B. thuringiensis genes and their encoded crystal proteins, as well as methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
摘要翻译： 公开了包含对鳞翅目昆虫具有杀虫活性的新型晶体蛋白的苏云金芽孢杆菌菌株。 还公开了新型苏云金芽孢杆菌基因及其编码的晶体蛋白质，以及制备和使用包含本发明新核酸序列的转基因细胞的方法。
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公开(公告)号：US4469027A
公开(公告)日：1984-09-04
申请号：US485344
申请日：1983-04-15
申请人： Bruce Burns , William Donovan
发明人： Bruce Burns , William Donovan
CPC分类号： F42B14/061 , F42B14/062
摘要： An ammunition round for an artillery gun comprises, as an armor piercing nd, an outer tubular disposable sabot and an inner projectile which are joined by interlocking means to join them until exiting from the gun muzzle and separates them in ballistic flight. The interlocking means comprises left-handed threads and right-handed threads; both directions of threads being on both the inner wall of the sabot and the outer wall of the projectile. In one embodiment the two directions of threads are on axially tandem sections and in another embodiment they are on the same section, to form a herringbone pattern with raised diamond-shaped protrusions.
摘要翻译： 用于炮火的弹药包括作为穿甲圆的外套管状一次性炮弹和内射弹，其通过互锁装置连接以将其连接直到从枪口喷射并且在弹道飞行中分离。 互锁装置包括左旋螺纹和右旋螺纹; 螺纹的两个方向都在破坏物的内壁和射弹的外壁上。 在一个实施例中，螺纹的两个方向在轴向串联的部分上，并且在另一个实施例中，它们在相同的部分上，以形成具有凸起的菱形突起的人字形图案。
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公开(公告)号：US20050093923A1
公开(公告)日：2005-05-05
申请号：US10818883
申请日：2004-04-06
申请人： Brian Busch , Daniel Bybell , William Donovan , Felix Lin , Suhail Saquib , Jay Thornton
发明人： Brian Busch , Daniel Bybell , William Donovan , Felix Lin , Suhail Saquib , Jay Thornton
CPC分类号： H04N1/6033
摘要： Techniques are disclosed for performing color correction in a color printer. An RGB test image is printed to produce a printed calibration image. Colors in the printed calibration image are measured and compared to expected color values. A color corrector generates a printer lookup table (LUT) based on this comparison. The LUT may be generated based on models of the printer's print engine and previous LUTs. The color corrections are applied to subsequent print jobs to improve the quality of color output. Color measurement and correction may be performed using a closed-loop system contained within the printer that includes a color measurement device (such as a scanner) and a computer.
摘要翻译： 公开了用于在彩色打印机中执行颜色校正的技术。 打印RGB测试图像以产生打印的校准图像。 测量印刷校准图像中的颜色并将其与预期颜色值进行比较。 颜色校正器基于该比较生成打印机查找表（LUT）。 LUT可以基于打印机的打印引擎和先前的LUT的模型生成。 颜色校正应用于后续打印作业，以提高颜色输出的质量。 可以使用包含在包括彩色测量装置（例如扫描仪）和计算机的打印机内的闭环系统来执行颜色测量和校正。
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