公开(公告)号：US07157232B2

公开(公告)日：2007-01-02

申请号：US11020731

申请日：2004-12-21

申请人： Robin R. Miles ,  Phillip Belgrader ,  Christopher D. Fuller

发明人： Robin R. Miles ,  Phillip Belgrader ,  Christopher D. Fuller

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6825 ,  C12Q1/6823 ,  C12Q1/686 ,  G01N27/3276 ,  C12Q2565/629 ,  C12Q2565/607 ,  C12Q2563/137 ,  C12Q2563/116

摘要： Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.

摘要翻译： 阻抗测量用于检测PCR DNA扩增的终点。 一对间隔开的电极位于微流体通道的表面上，并且跨电极施加AC或DC电压以产生电场。 离子标记的探针将附着于互补DNA片段，聚合酶将释放离子标签。 这导致溶液在电极区域中的电导率改变。 电导率的这种变化被测量为两个电极的阻抗的变化。

公开(公告)号：US20060134616A1

公开(公告)日：2006-06-22

申请号：US11352108

申请日：2006-02-09

申请人： Phillip Belgrader ,  Bob Yuan

发明人： Phillip Belgrader ,  Bob Yuan

IPC分类号： C12Q1/70 ,  C12Q1/68 ,  C12N1/08

CPC分类号： C12N1/06 ,  B01L3/5025 ,  B01L3/5027 ,  B01L2200/0631 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12M47/06

摘要： A sonication apparatus is directed to a microfluidic-based system to automate differential extraction of specific cell types within a mixed sample. The microfluidic-based system includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and microfluidic circuitry to integrate the steps in an automated platform.

公开(公告)号：US20050136466A1

公开(公告)日：2005-06-23

申请号：US11020731

申请日：2004-12-21

申请人： Robin Miles ,  Phillip Belgrader ,  Christopher Fuller

发明人： Robin Miles ,  Phillip Belgrader ,  Christopher Fuller

IPC分类号： C12M1/34 ,  C12P19/34 ,  C12Q1/68 ,  G01N27/02

CPC分类号： C12Q1/6825 ,  C12Q1/6823 ,  C12Q1/686 ,  G01N27/3276 ,  C12Q2565/629 ,  C12Q2565/607 ,  C12Q2563/137 ,  C12Q2563/116

摘要： Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.

摘要翻译： 阻抗测量用于检测PCR DNA扩增的终点。 一对间隔开的电极位于微流体通道的表面上，并且跨电极施加AC或DC电压以产生电场。 离子标记的探针将附着于互补DNA片段，聚合酶将释放离子标签。 这导致溶液在电极区域中的电导率改变。 电导率的这种变化被测量为两个电极的阻抗的变化。

公开(公告)号：US6100084A

公开(公告)日：2000-08-08

申请号：US187162

申请日：1998-11-05

申请人： Robin R. Miles ,  Phillip Belgrader ,  Shanavaz L. Nasarabadi

发明人： Robin R. Miles ,  Phillip Belgrader ,  Shanavaz L. Nasarabadi

IPC分类号： C12M1/33 ,  C12M3/08

CPC分类号： C12M47/06 ,  Y10S435/82

摘要： A micro-sonicator for spore lysis. Using micromachining technology, the micro-sonicator uses ultrasonic excitation of spores to perform spore and cell lysis. The micro-sonicator comprises a container with a cavity therein for retaining the sample in an ultrasonic transmission medium, the cavity being closed by a silicon membrane to which an electrode and piezoelectric material are attached, with the electrode and piezoelectric material being electrically connected to an AC signal generator which causes the membrane to flex and vibrate at the frequency of the applied voltage.

摘要翻译： 用于孢子裂解的微型超声波仪。 使用微加工技术，微超声波仪使用超声波激发孢子进行孢子和细胞裂解。 微型超声波器包括一个容器，其中有一个腔体，用于将样品保持在超声波传播介质中，该腔体被一个硅膜封闭，一个电极和压电材料被连接到硅膜上，电极和压电材料电连接到一个 AC信号发生器，其使得膜以施加电压的频率弯曲和振动。