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1.发明授权Method to detect the end-point for PCR DNA amplification using an ionically labeled probe and measuring impedance change 失效使用离子标记的探针检测PCR DNA扩增的终点并测量阻抗变化的方法公开(公告)号：US07157232B2
公开(公告)日：2007-01-02
申请号：US11020731
申请日：2004-12-21
CPC分类号： C12Q1/6825 , C12Q1/6823 , C12Q1/686 , G01N27/3276 , C12Q2565/629 , C12Q2565/607 , C12Q2563/137 , C12Q2563/116
摘要： Impedance measurements are used to detect the end-point for PCR DNA amplification. A pair of spaced electrodes are located on a surface of a microfluidic channel and an AC or DC voltage is applied across the electrodes to produce an electric field. An ionically labeled probe will attach to a complementary DNA segment, and a polymerase enzyme will release the ionic label. This causes the conductivity of the solution in the area of the electrode to change. This change in conductivity is measured as a change in the impedance been the two electrodes.
摘要翻译： 阻抗测量用于检测PCR DNA扩增的终点。 一对间隔开的电极位于微流体通道的表面上，并且跨电极施加AC或DC电压以产生电场。 离子标记的探针将附着于互补DNA片段，聚合酶将释放离子标签。 这导致溶液在电极区域中的电导率改变。 电导率的这种变化被测量为两个电极的阻抗的变化。
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公开(公告)号：US6100084A
公开(公告)日：2000-08-08
申请号：US187162
申请日：1998-11-05
CPC分类号： C12M47/06 , Y10S435/82
摘要： A micro-sonicator for spore lysis. Using micromachining technology, the micro-sonicator uses ultrasonic excitation of spores to perform spore and cell lysis. The micro-sonicator comprises a container with a cavity therein for retaining the sample in an ultrasonic transmission medium, the cavity being closed by a silicon membrane to which an electrode and piezoelectric material are attached, with the electrode and piezoelectric material being electrically connected to an AC signal generator which causes the membrane to flex and vibrate at the frequency of the applied voltage.
摘要翻译： 用于孢子裂解的微型超声波仪。 使用微加工技术，微超声波仪使用超声波激发孢子进行孢子和细胞裂解。 微型超声波器包括一个容器，其中有一个腔体，用于将样品保持在超声波传播介质中，该腔体被一个硅膜封闭，一个电极和压电材料被连接到硅膜上，电极和压电材料电连接到一个 AC信号发生器，其使得膜以施加电压的频率弯曲和振动。
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公开(公告)号：US10300482B2
公开(公告)日：2019-05-28
申请号：US13314734
申请日：2011-12-08
申请人： Christopher G. Cooney , Phillip Belgrader , Arial Bueno , Steve Garber , Nitu Harshendu Thakore , Peter Qiang Qu
发明人： Christopher G. Cooney , Phillip Belgrader , Arial Bueno , Steve Garber , Nitu Harshendu Thakore , Peter Qiang Qu
摘要： An integrated sample analysis system is disclosed. The sample analysis system contains (1) a sample preparation/analysis module having sample purification device comprising a monolith that binds specifically to nucleic acids and a sample analysis device comprising a microarray enclosed in a reaction chamber having a hydrophilic interior surface; (2) a temperature control module comprising a thermocycler having a thermally conductive temperature-control bladder; and (3) an imaging device capable of capturing an image of the microarray in the reaction chamber.
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公开(公告)号：US08580559B2
公开(公告)日：2013-11-12
申请号：US11977615
申请日：2007-10-24
申请人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
发明人： Kurt E. Petersen , Michael T. Taylor , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Stanley H. Sakai , Jesus Ching , Douglas B. Dority , Phillip Belgrader , M. Allen Northrup
IPC分类号： C12M1/34
CPC分类号： B01L3/502 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0681 , B01L2300/0809 , B01L2300/087 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , C12N1/066 , C12N13/00 , Y10S435/81 , Y10S436/805 , Y10S436/806 , Y10S436/807 , Y10S436/809 , Y10S436/829 , Y10T137/87716 , Y10T137/88046 , Y10T436/255
摘要： The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
摘要翻译： 本发明提供一种用于分析流体样品的盒。 盒提供用于有效分离样品中的细胞或病毒与剩余的样品流体，裂解细胞或病毒以从其中分离分析物（例如核酸），以及任选地分析物的化学反应和/或检测。 墨盒可用于各种诊断，生命科学研究，环境或法医应用，用于确定样品中一种或多种分析物的存在或不存在。
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公开(公告)号：US20130079253A1
公开(公告)日：2013-03-28
申请号：US13542111
申请日：2012-07-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01L3/502715 , B01L2200/026 , B01L2200/10 , B01L2300/0681 , G01N1/34
摘要： A microarray-based sample analysis (MBSA) system includes a cartridge holder adapted to receive a replaceable cartridge that is configured to receive a detachable, replaceable sample analysis unit containing one or more reaction chambers for sample analysis; a fluid control subsystem that controls fluid flow; and an optical subsystem configured to capture an image of the microarray.
摘要翻译： 基于微阵列的样本分析（MBSA）系统包括适于接收替换的盒的盒保持器，其被配置为接收包含用于样本分析的一个或多个反应室的可拆卸的可更换样品分析单元; 控制流体流动的流体控制子系统; 以及被配置为捕获微阵列的图像的光学子系统。
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公开(公告)号：US08236501B2
公开(公告)日：2012-08-07
申请号：US12793218
申请日：2010-06-03
申请人： Phillip Belgrader
发明人： Phillip Belgrader
CPC分类号： G01N1/405 , B01D39/201 , B01L3/0275 , B01L3/502753 , B01L2200/0631 , B01L2300/0681 , C12N15/1017
摘要： Methods and devices for isolating nucleic acids from a mixture containing such nucleic acids and extraneous matter are provided. In one embodiment, the method of the invention comprises passing the mixture through a glass frit under conditions effective to separate the nucleic acids from the extraneous matter. In a more specific embodiment, the glass frit is a sintered glass fit.
摘要翻译： 提供了从含有这种核酸和外来物质的混合物中分离核酸的方法和装置。 在一个实施方案中，本发明的方法包括在有效将核酸与外来物质分离的条件下使混合物通过玻璃料。 在更具体的实施例中，玻璃料是烧结玻璃。
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公开(公告)号：US20120149872A1
公开(公告)日：2012-06-14
申请号：US12003416
申请日：2007-12-26
申请人： Phillip Belgrader
发明人： Phillip Belgrader
CPC分类号： C12N15/1006 , B01D15/161 , B01D17/0202 , B01L3/502753 , B01L3/5029 , B01L7/52 , B01L2200/027 , B01L2200/0647 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/0883 , B01L2300/16 , B01L2300/1827 , B01L2400/0487 , C07K1/22 , C07K1/36 , G01N2030/8831
摘要： The present invention relates to a device for purifying an analyte from a fluid sample. The device comprises a channel or tubing having an inner surface that binds to the analyte of interest in the fluid sample. As the fluid sample flows through the channel, the analyte of interest binds to the inner wall of the channel. The bound analyte is then eluted using a small bolus of elution buffer. The channel generates a high surface area for capturing the analyte in a large volume sample, but allows low liquid elution volume for concentrating the analyte into a small volume.
摘要翻译： 本发明涉及一种用于从流体样品中纯化分析物的装置。 该装置包括具有与流体样品中感兴趣的分析物结合的内表面的通道或管道。 当流体样品流过通道时，感兴趣的分析物与通道的内壁结合。 然后使用小剂量的洗脱缓冲液洗脱结合的分析物。 该通道产生用于捕获大体积样品中的分析物的高表面积，但允许低液体洗脱体积用于将分析物浓缩成小体积。
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公开(公告)号：US20100284859A1
公开(公告)日：2010-11-11
申请号：US12840826
申请日：2010-07-21
IPC分类号： G01N33/00
CPC分类号： B01J19/0046 , B01J2219/00612 , B01J2219/0063 , B01J2219/00637 , B01J2219/00644 , B01J2219/00722 , B01J2219/00725 , B01L3/502723 , B01L3/50273 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/165 , B01L2400/0406 , B01L2400/0688
摘要： A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.
摘要翻译： 公开了一种微阵列系统。 微阵列系统包括形成在平面基底上的微阵列和形成在微阵列周围的孵育室。 孵育室具有多个内表面，其包括形成微阵列的底表面和面向底表面并且大致平行于底表面的顶表面。 多个内表面中的至少一个是亲水表面。
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公开(公告)号：US07097980B2
公开(公告)日：2006-08-29
申请号：US10852289
申请日：2004-05-24
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
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公开(公告)号：US06987018B2
公开(公告)日：2006-01-17
申请号：US10208976
申请日：2002-07-30
申请人： Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Douglas B. Dority
发明人： Michael T. Taylor , Phillip Belgrader , Farzad Pourahmadi , William A. McMillan , Ronald Chang , Douglas B. Dority
IPC分类号： C12M1/34
CPC分类号： B01L3/502 , B01L3/50825 , B01L7/52 , B01L2300/042 , B01L2300/046 , B01L2300/14 , B01L2400/0439 , B01L2400/0622 , B01L2400/0644 , C12M47/06
摘要： A container for holding cells or viruses for disruption comprises a chamber defined by two spaced apart, opposing major walls and side walls connecting the major walls to each other. At least one of the major walls has an external surface to which the transducer may be coupled and is sufficiently flexible to flex in response to vibratory motion of the transducer. The container also has at least one port for introducing the cells or viruses into the chamber. In some embodiments, the chamber contains beads for aiding the disruption of the cells or viruses.
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