公开(公告)号：US20220214279A1

公开(公告)日：2022-07-07

申请号：US17704691

申请日：2022-03-25

Applicant: Quantum-Si Incorporated

Inventor： JONATHAN M. ROTHBERG ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Ciperiany ,  Jack Jewell ,  Lawrence C. West ,  Michael Ferrigno ,  Paul E. Glenn ,  Adam Ezra Cohen ,  Anthony Bellofiore

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/77 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

公开(公告)号：US20220120685A1

公开(公告)日：2022-04-21

申请号：US17497827

申请日：2021-10-08

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Paul E. Glenn ,  Lawrence C. West ,  Benjamin Cipriany ,  Keith G. Fife

IPC: G01N21/64 ,  C12Q1/6874

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

公开(公告)号：US11175227B2

公开(公告)日：2021-11-16

申请号：US16442861

申请日：2019-06-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Paul E. Glenn ,  Lawrence C. West ,  Benjamin Cipriany ,  Keith G. Fife

IPC: G01N21/64 ,  C12Q1/6874

Abstract: Apparatus and methods for analyzing single molecules and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

公开(公告)号：US10712273B2

公开(公告)日：2020-07-14

申请号：US15960751

申请日：2018-04-24

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: G01N21/00 ,  G01N21/64 ,  C12Q1/6874 ,  G01N21/77 ,  B01L3/00 ,  C12Q1/6869

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

公开(公告)号：US20200088643A1

公开(公告)日：2020-03-19

申请号：US16689004

申请日：2019-11-19

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence C. West ,  Michael Ferrigno ,  Paul E. Glenn ,  Adam Ezra Cohen ,  Anthony Bellofiore

IPC: G01N21/64 ,  C12Q1/6869 ,  B01L3/00 ,  G01N21/77 ,  C12Q1/6874

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

公开(公告)号：US20180348133A1

公开(公告)日：2018-12-06

申请号：US15974826

申请日：2018-05-09

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: G01N21/64 ,  B01L3/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/77

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

公开(公告)号：US10048208B2

公开(公告)日：2018-08-14

申请号：US14543865

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Anthony Bellofiore

IPC: G01N21/00 ,  G01N21/64 ,  C12Q1/6874 ,  G01N21/77 ,  C12Q1/6869

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

公开(公告)号：US09983135B2

公开(公告)日：2018-05-29

申请号：US14543888

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid

IPC: C12Q1/68 ,  G01N21/64 ,  G01N21/77

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: An active-source-pixel, integrated device capable of performing biomolecule detection and/or analysis, such as single-molecule nucleic acid sequencing, is described. An active pixel of the integrated device includes a sample well into which a sample to be analyzed may diffuse, an excitation source for providing excitation energy to the sample well, and a sensor configured to detect emission from the sample. The sensor may comprise two or more segments that produce a set of signals that are analyzed to differentiate between and identify tags that are attached to, or associated with, the sample. Tag differentiation may be spectral and/or temporal based. Identification of the tags may be used to detect, analyze, and/or sequence the biomolecule.

公开(公告)号：US09863880B2

公开(公告)日：2018-01-09

申请号：US14543867

申请日：2014-11-17

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Benjamin Cipriany ,  Jack Jewell ,  Lawrence West ,  Michael Ferrigno ,  Paul E. Glenn ,  Adam E. Cohen ,  Anthony Bellofiore

IPC: C12M1/00 ,  G01N21/64 ,  C12Q1/68 ,  G01N21/77

CPC classification number: G01N21/6486 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/168 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2565/607 ,  G01N21/64 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/648 ,  G01N21/7743 ,  G01N21/7746 ,  G01N2021/6419 ,  G01N2021/6441 ,  G01N2021/6478 ,  G01N2201/02 ,  G01N2201/06113 ,  G01N2201/0612 ,  G01N2201/062 ,  G01N2201/068 ,  G01N2201/125 ,  Y10T29/49016 ,  C12Q2525/101

Abstract: Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An apparatus can include an assay chip that includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits emission energy; at least one element for directing the emission energy in a particular direction; and a light path along which the emission energy travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the assay chip. The instrument includes an excitation light source for exciting the sample in each sample well; a plurality of sensors corresponding the sample wells. Each sensor may detect emission energy from a sample in a respective sample well. The instrument includes at least one optical element that directs the emission energy from each sample well towards a respective sensor of the plurality of sensors.

公开(公告)号：US20170299518A1

公开(公告)日：2017-10-19

申请号：US15641949

申请日：2017-07-05

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Ali Kabiri ,  Jason W. Sickler ,  Brett J. Gyarfas ,  Jeremy Lackey ,  Gerard Schmid ,  Lawrence C. West ,  Keith G. Fife ,  Benjamin Cipriany ,  Farshid Ghasemi

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6408 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6454 ,  G01N21/648 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/0696 ,  G01N2201/08 ,  G01N2201/12

Abstract: System and methods for analyzing single molecules and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which when excited, emits radiation. The integrated device includes at least one waveguide configured to propagate excitation energy to the sample wells from a region of the integrated device configured to couple with an excitation energy source. A pixel may also include at least one element for directing the emission energy towards a sensor within the pixel. The system also includes an instrument that interfaces with the integrated device. The instrument may include an excitation energy source for providing excitation energy to the integrated device by coupling to an excitation energy coupling region of the integrated device. One of multiple markers distinguishable by temporal parameters of the emission energy may label the sample and configuration of the sensor within a pixel may allow for detection of a temporal parameter associated with the marker labeling the sample.