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    Alphavirus RNA replicon systems
    42.
    发明授权
    Alphavirus RNA replicon systems 有权
    甲病毒RNA复制子系统

    公开(公告)号：US07235235B2

    公开(公告)日：2007-06-26

    申请号：US10388327

    申请日：2003-03-13

    Applicant: Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    Inventor： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    IPC: A01N63/00 ,  A61K39/12 ,  C12N7/00 ,  C12N7/04 ,  C12N7/02 ,  C12N5/00

    CPC classification number: C07K14/005 ,  A61K2039/5254 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162 ,  C12N2840/20

    Abstract: The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least alphavirus one structural protein not encoded by the first helper RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell. Preferably, the helper cell also includes a replicon RNA encoding an alphavirus packaging sequence and an inserted heterogeneous RNA.

    Abstract translation: 本发明提供了用于在甲病毒允许细胞中表达感染性，复制缺陷型甲病毒颗粒的辅助细胞。 辅助细胞包括（a）编码（i）至少一种甲病毒结构蛋白的第一辅助RNA，和（ii）不编码至少一种甲病毒属结构蛋白; 和（b）与第一辅助RNA分开的第二辅助RNA，不编码由第一辅助RNA编码的甲病毒结构蛋白的第二辅助RNA（i），和（ii）编码至少一种不是由第一辅助RNA编码的结合蛋白的结构蛋白， 第一辅助RNA，使得所有甲病毒结构蛋白聚集在细胞中的甲病毒颗粒中。 优选地，辅助细胞还包括编码甲病毒包装序列和插入的异质RNA的复制子RNA。

    Alphavirus RNA replicon systems
    44.
    发明授权
    Alphavirus RNA replicon systems 有权
    甲病毒RNA复制子系统

    公开(公告)号：US06521235B2

    公开(公告)日：2003-02-18

    申请号：US09803600

    申请日：2001-03-09

    Applicant: Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    Inventor： Robert E. Johnston ,  Nancy L. Davis ,  Jonathan F. Smith ,  Peter Pushko ,  Michael Parker ,  George Ludwig

    IPC: A61K3912

    CPC classification number: C07K14/005 ,  A61K2039/5254 ,  C12N7/00 ,  C12N15/86 ,  C12N2770/36122 ,  C12N2770/36143 ,  C12N2770/36152 ,  C12N2770/36162 ,  C12N2840/20

    Abstract: The present invention provides a helper cell for expressing an infectious, replication defective, alphavirus particle in an alphavirus-permissive cell. The helper cell includes (a) a first helper RNA encoding (i) at least one alphavirus structural protein, and (ii) not encoding at least one alphavirus structural protein; and (b) a second helper RNA separate from the first helper RNA, the second helper RNA (i) not encoding the alphavirus structural protein encoded by the first helper RNA, and (ii) encoding the at least one alphavirus structural protein not encoded by the first helper RNA. Preferably, the helper cell is co-transfected with a replicon RNA encoding an alphavirus packaging segment and an inserted heterogeneous RNA, such that all of the alphavirus structural proteins assemble together into alphavirus particles in the cell, with said replicon RNA packaged therein.

    Abstract translation: 本发明提供了用于在甲病毒允许细胞中表达感染性，复制缺陷型甲病毒颗粒的辅助细胞。 辅助细胞包括（a）编码（i）至少一种甲病毒结构蛋白的第一辅助RNA，和（ii）不编码至少一种甲病毒属结构蛋白; 和（b）与第一辅助RNA分开的第二辅助RNA，不编码由第一辅助RNA编码的甲病毒结构蛋白的第二辅助RNA（i），和（ii）编码不由第一辅助RNA编码的至少一种甲病毒结构蛋白， 第一个辅助RNA。 优选地，辅助细胞与编码甲病毒包装片段和插入的异质RNA的复制子RNA共转染，使得所有甲病毒属结构蛋白在一起组装成细胞中的甲病毒颗粒，其中包含所述复制子RNA。

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