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54 条记录 (耗时 0.092 秒)

    Multiply Fluorescently Labeled Calcium Phosphate-Protein Surfaces
    42.
    发明申请
    Multiply Fluorescently Labeled Calcium Phosphate-Protein Surfaces 审中-公开
    倍增荧光标记的磷酸钙 - 蛋白质表面

    公开(公告)号：US20110294142A1

    公开(公告)日：2011-12-01

    申请号：US12846209

    申请日：2010-07-29

    申请人： Joydeep Lahiri ,  Hongwei Hanna Rao ,  Jian Tan

    发明人： Joydeep Lahiri ,  Hongwei Hanna Rao ,  Jian Tan

    IPC分类号： G01N33/574 ,  B05D1/36 ,  G01N33/50

    CPC分类号： C12N5/0068 ,  C12N5/0643 ,  C12N2533/18 ,  C12N2533/54 ,  G01N33/533

    摘要： Described herein are multiply-fluorescently-labeled calcium phosphate protein substrates composed of a base coated with a fluorophore-labeled calcium phosphate coating and further having a fluorescently-labeled protein. The multiply-fluorescently-labeled substrate may be a fluorescently labeled calcium phosphate surface having a fluorescently-labeled collagen. The substrates are useful in culturing and studying the activity of a variety of cells, including bone cells. The substrates described herein can be used for both solution- and image-based analysis of cultured cells. New methods for producing and using such coated substrates are also disclosed.

    摘要翻译： 本文描述的是多重荧光标记的磷酸钙蛋白质底物，其由涂布有荧光团标记的磷酸钙涂层的基底组成，并且还具有荧光标记的蛋白质。 多荧光标记的底物可以是具有荧光标记的胶原蛋白的荧光标记的磷酸钙表面。 底物可用于培养和研究包括骨细胞在内的各种细胞的活性。 本文所述的底物可用于培养细胞的基于溶液和图像的分析。 还公开了用于生产和使用这种涂覆的基材的新方法。

    Variable Penetration Depth Biosensor Methods
    44.
    发明申请
    Variable Penetration Depth Biosensor Methods 失效
    可变穿透深度生物传感器方法

    公开(公告)号：US20110171746A1

    公开(公告)日：2011-07-14

    申请号：US12627463

    申请日：2009-11-30

    申请人： Norman Henry Fontaine ,  Joydeep Lahiri ,  Guangshan Li ,  Anping Liu ,  Jinlin Peng

    发明人： Norman Henry Fontaine ,  Joydeep Lahiri ,  Guangshan Li ,  Anping Liu ,  Jinlin Peng

    IPC分类号： G01N21/55

    CPC分类号： G01N21/553

    摘要： A surface plasmon resonance sensor system including a high refractive index prism, a sensor chip, a light source having multiple wavelengths over a broad range of wavelengths, optical lenses, a photodetector, a data acquisition unit, and as defined herein. The sensor chip can include, for example, a thin layer of silicon and gold on one face of a transparent substrate and the prism adjacent to the opposite face of the transparent substrate. Such an arrangement provides variable penetration depths up to about 1.5 micrometers with a dynamic range for sensing index of refraction changes in a sample that are several times greater than that of a conventional SPR sensor. The disclosure provides methods for using the surface plasmon resonance sensor system for cell assay or chemical assay related applications.

    摘要翻译： 一种表面等离子体共振传感器系统，包括高折射率棱镜，传感器芯片，在宽波长范围内具有多个波长的光源，光学透镜，光电检测器，数据采集单元，如本文所定义。 传感器芯片例如可以包括在透明基板的一个面上的硅和金的薄层和邻近透明基板的相对面的棱镜。 这种布置提供高达约1.5微米的可变穿透深度，具有用于感测样品中的折射改变的动态范围，其比常规SPR传感器的折射几倍大。 本公开提供了使用表面等离子体共振传感器系统用于细胞测定或化学测定相关应用的方法。

    Live-cell signals of pathogen intrusion and methods thereof
    46.
    发明申请
    Live-cell signals of pathogen intrusion and methods thereof 审中-公开
    病原体入侵的活细胞信号及其方法

    公开(公告)号：US20100323902A1

    公开(公告)日：2010-12-23

    申请号：US12080765

    申请日：2008-04-04

    申请人： Ye Fang ,  Joydeep Lahiri ,  Florence Verrier

    发明人： Ye Fang ,  Joydeep Lahiri ,  Florence Verrier

    IPC分类号： C40B30/00 ,  G01N33/53

    CPC分类号： G01N33/54373 ,  G01N21/553 ,  G01N33/569

    摘要： Disclosed is a system and method for measuring aspects of pathogen intrusion on a live-cell as defined herein. The system and method also provide a method to measure prophylaxis or remedial aspects of a therapeutic candidates in a live-cell or a live-cell model from pathogen intrusion.

    摘要翻译： 公开了用于测量如本文所定义的活细胞上的病原体侵入方面的系统和方法。 该系统和方法还提供了一种用于测量来自病原体入侵的活细胞或活细胞模型中的治疗候选物的预防或补救方面的方法。

    SUBSTRATE AND METHOD FOR CULTURING BREAST CELLS
    48.
    发明申请
    SUBSTRATE AND METHOD FOR CULTURING BREAST CELLS 审中-公开
    用于培养乳腺细胞的基质和方法

    公开(公告)号：US20100297675A1

    公开(公告)日：2010-11-25

    申请号：US12623884

    申请日：2009-11-23

    申请人： Huayun Deng ,  Joydeep Lahiri ,  Hui Su

    发明人： Huayun Deng ,  Joydeep Lahiri ,  Hui Su

    IPC分类号： G01N33/574 ,  C12N5/07

    CPC分类号： C12N5/0631 ,  C12N5/0693 ,  C12N2503/02 ,  C12N2506/30 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2535/00 ,  G01N33/5011 ,  G01N33/57415

    摘要： A cell culture article includes a porous substrate having a plurality of pores and a plurality of interstices in communication with the pores. At least some of the plurality of pores and interstices are sufficiently large for two or more mammary epithelial cells to cluster within the pores or interstices. Non-malignant mammary epithelial cells or breast cancer cells may not attach strongly to the substrate surface, which may encourage cell-cell interaction. In many cases, the article is desirably free of components of unknown origin. The articles may be capable of maintaining culture of malignant and non-malignant mammary epithelial cells and allowing for development of in vivo-like morphologies or characteristics of such cells.

    摘要翻译： 细胞培养物包括具有多个孔和与孔连通的多个间隙的多孔基材。 多个孔和间隙中的至少一些孔和间隙对于两个或更多个乳腺上皮细胞聚集在孔或间隙内是足够大的。 非恶性乳腺上皮细胞或乳腺癌细胞可能不会强烈附着于底物表面，这可能促进细胞 - 细胞相互作用。 在许多情况下，该物品理想地不含未知来源的成分。 这些制品可能能够维持恶性和非恶性乳腺上皮细胞的培养，并允许发展体内样的形态或这些细胞的特征。

    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors
    50.
    发明申请
    System and Method for Performing G Protein Coupled Receptor (GPCR) Cell Assays Using Waveguide-Grating Sensors 有权
    使用波导光栅传感器进行G蛋白偶联受体（GPCR）细胞测定的系统和方法

    公开(公告)号：US20090275074A1

    公开(公告)日：2009-11-05

    申请号：US12469054

    申请日：2009-05-20

    申请人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    发明人： Ye Fang ,  Ann M. Ferrie ,  Norman H. Fontaine ,  Joydeep Lahiri ,  Po Ki Yuen

    IPC分类号： C12Q1/02

    CPC分类号： G01N33/5041 ,  G01N21/4788 ,  G01N21/553 ,  G01N2333/726 ,  G01N2500/10

    摘要： The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.

    摘要翻译： 本发明包括使用光学LID生物传感器实时监测活细胞内激动剂诱导的GPCR信号事件的系统和方法。 特别地，本发明包括使用光学LID生物传感器基于由于激动剂诱导的GPCR活化导致的质量再分布来筛选活细胞内的靶GPCR的化合物的系统和方法。 在扩展实施例中，本发明公开了用于自参考光学LID生物传感器以消除对环境温度，压力波动和其他环境变化的不期望的灵敏度的不同方式。 在又一个扩展的实施方案中，本发明公开了在单一培养基溶液中的多种类型的细胞中单种类型细胞或多种GPCR筛选多种GPCR的不同方式。 在又一个延伸的实施方案中，本发明公开了确定化合物对活细胞内特异性GPCR的生理或药理作用的不同方式。