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公开(公告)号：US4861706A
公开(公告)日：1989-08-29
申请号：US857695
申请日：1986-04-29
CPC分类号： C12Q1/706 , Y10S435/948
摘要： A quantitative assay for HAV comprises infecting at a temperature of from about 30.degree. to about 37.degree. C. a cell sheet with a dilution series of hepatitis A virus and then adding Newcastle disease virus (NDV) to the infected cell sheet and incubating the infected cell sheet in the presence of NDV at an elevated temperature for a time sufficient to permit a cytopathic effect (CPE) to become manifest, this time typically being at least about 4 days and preferably about 5 days. At an incubation temperature of from about 31.degree. to about 33.degree. C. the absence of CPE indicates the presence of HAV and the presence of CPE indicates the absence of HAV. At an incubation temperature of from about 34.degree. to about 36.degree. C. the absence of CPE indicates the absence of HAV and the presence of CPE indicates the presence of HAV.
摘要翻译： 用于HAV的定量测定包括在约30℃至约37℃的温度下感染具有稀释系列的甲型肝炎病毒的细胞片，然后向感染的细胞片中加入新城疫病毒（NDV）并孵育感染的 细胞片在NDV存在下在升高的温度下持续足以允许细胞病变效应（CPE）变得明显的时间，此时通常为至少约4天，优选约5天。 在约31℃至约33℃的温育温度下，不存在CPE表明存在HAV，而CPE的存在表明不存在HAV。 在约34℃至约36℃的培养温度下，不存在CPE表明不存在HAV，且CPE的存在表明存在HAV。
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公开(公告)号：US4783407A
公开(公告)日：1988-11-08
申请号：US781830
申请日：1985-09-30
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , C12N2770/32434 , C12N2770/32464
摘要： Hepatitus A virus is grown in Vero cells after passage in in vitro cell culture. Virus replication and continued passage in Vero cells requires extended incubation times, up to about four weeks, for early passages and incubation temperatures no higher than about 32.degree. C. Continued passage results in a significant decrease in incubation time and an increase in virus yield. Cultivation of hepatitus A virus in Vero cells meets the development requirements for an inactivated human vaccine.
摘要翻译： Hepatitus A病毒在体外细胞培养物中通过后在Vero细胞中生长。 Vero细胞中的病毒复制和持续传代需要延长的孵育时间，长达约4周，用于早期传代和孵育温度不高于约32℃。连续传代导致孵育时间的显着降低和病毒产量的增加。 Vero细胞中肝病毒A的培养符合灭活人类疫苗的发展要求。
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公开(公告)号：US4338335A
公开(公告)日：1982-07-06
申请号：US173534
申请日：1980-07-30
IPC分类号： A61K39/12 , A61K39/165 , A61K39/13 , A61K39/245
CPC分类号： A61K39/165 , A61K39/12 , C12N7/00 , A61K2039/525 , A61K2039/5254 , C12N2760/18434 , C12N2760/18451
摘要： An improved stabilized liquid live viral vaccine contains a live virus, partially hydrolyzed gelatin, a monosaccharide or disaccharide, a cell culture medium, L-glutamic acid, L-arginine and sufficient physiologically acceptable acidic buffer to maintain the pH at from about 6.0 to about 6.5.
摘要翻译： 改进的稳定的液体活病毒疫苗含有活病毒，部分水解的明胶，单糖或二糖，细胞培养基，L-谷氨酸，L-精氨酸和足够的生理上可接受的酸性缓冲液，以将pH保持在约6.0至约 6.5。
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公开(公告)号：US4319271A
公开(公告)日：1982-03-09
申请号：US970722
申请日：1978-12-18
CPC分类号： C12Q1/70 , G01N21/253
摘要： A method for determining the end point in a viral assay comprisingelectronically scanning a microtiter plate to obtain a plurality of data points from each well,transmitting the data points to a digital computer programed to analyze the data points from each well to determine whether each well is positive or negative for CPE in accordance with predetermined parameters, andcalculating the viral titer from the positive or negative results for each well.
摘要翻译： 一种用于确定病毒测定中的终点的方法，包括电子扫描微量滴定板以从每个孔获得多个数据点，将数据点传送到数字计算机，该数字计算机被编程以分析来自每个孔的数据点，以确定每个孔 根据预定参数对于CPE是阳性或阴性，并且从每个孔的阳性或阴性结果计算病毒滴度。
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公开(公告)号：US4301249A
公开(公告)日：1981-11-17
申请号：US171621
申请日：1980-07-23
CPC分类号： A61K39/29 , A61K39/12 , C12N2770/32434 , C12N2770/32451
摘要： Hepatitis A virus (HAV) is produced in high titer from tissue cultures grown on a hollow fiber capillary unit.
摘要翻译： 从生长在中空纤维毛细管单元上的组织培养物以高效价产生甲型肝炎病毒（HAV）。
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公开(公告)号：US4115010A
公开(公告)日：1978-09-19
申请号：US655318
申请日：1976-02-03
CPC分类号： G01N21/253
摘要： Apparatus and method for automatically reading a microtiter plate comprises a light source, a photo sensor, means enabling the photo sensor to measure light transmittance and means for recording and processing the measurements.
摘要翻译： 用于自动读取微量滴定板的装置和方法包括光源，光传感器，使得光传感器能够测量透光率的装置和用于记录和处理测量的装置。
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公开(公告)号：US4110223A
公开(公告)日：1978-08-29
申请号：US744228
申请日：1976-11-23
CPC分类号： C12M21/10 , A47J43/145 , C12M33/00
摘要： A method and apparatus for recovering embryos from embryonated eggs wherein the egg is cracked open the shell discarded and the contents dropped onto a continuously vibrating screen which separates the embryo from the yolk sac and accompanying fluid. This associated material, which is less cohesive than the embryo, passes through the screen. The screen itself is tilted at a compound angle from the horizontal and its vibrating action causes the embryos to move along the upper surface of the screen toward a discharge chute located at the lower corner of the screen.
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公开(公告)号：US4018222A
公开(公告)日：1977-04-19
申请号：US577244
申请日：1975-05-13
摘要： Vaccine delivery system wherein an aqueous vaccine diluted to human dosage concentration is loaded into presterilized single dosage syringes having a squeezable body portion and wherein a plurality of these syringes are sealed inside a bag and the contents of the syringes are frozen to preserve the titer of the vaccine.
摘要翻译： 疫苗递送系统，其中将稀释至人的剂量浓度的水性疫苗装载到具有可挤压主体部分的预灭灭的单剂量注射器中，并且其中将多个这些注射器密封在袋内，并将注射器的内容物冷冻以保持 疫苗。
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公开(公告)号：US3976547A
公开(公告)日：1976-08-24
申请号：US525270
申请日：1974-11-19
CPC分类号： C12N5/0068 , C12N2533/10
摘要： Cell growth surfaces having controlled surface topography which permits the optimization of the fit between the cells and the growth surface. These surfaces may be constructed from sintered or sputtered titanium, glass, ceramic, plastic, or stainless steel, or may consist of a substrate having microspheres adhered to the surface thereof.
摘要翻译： 具有受控表面形貌的细胞生长表面，其允许优化细胞与生长表面之间的配合。 这些表面可以由烧结或溅射的钛，玻璃，陶瓷，塑料或不锈钢构成，或者可以由具有附着在其表面上的微球的基底组成。
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