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137 条记录 (耗时 0.053 秒)

    Methods for detection and quantification of analytes in complex mixtures
    41.
    发明授权
    Methods for detection and quantification of analytes in complex mixtures 有权
    复杂混合物中分析物的检测和定量方法

    公开(公告)号：US08148512B2

    公开(公告)日：2012-04-03

    申请号：US12819101

    申请日：2010-06-18

    申请人： Krassen M. Dimitrov

    发明人： Krassen M. Dimitrov

    IPC分类号： C07H21/04 ,  C12Q1/68

    CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6876 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2525/161 ,  C12Q2565/1025 ,  C12Q2563/179 ,  C12Q2525/151 ,  C12Q2525/101

    摘要： The invention provides a diverse population of uniquely labeled probes, containing target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of antigenedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.

    摘要翻译： 本发明提供了多种独特标记的探针，其含有目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。 还提供了检测核酸分析物的方法。 该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗原多肽杂交的条件下使混合物接触，所述多个抗Genetigits具有足以独特地与指定剂内的genesigits杂交的多样性，以及（c）唯一地检测杂交的 混合物中一种或多种分析物之间的复合物，目标特异性探针和抗Genetigigit。

    Multiparameter analysis for predictive medicine
    42.
    发明授权
    Multiparameter analysis for predictive medicine 有权
    预测医学多参数分析

    公开(公告)号：US08036834B2

    公开(公告)日：2011-10-11

    申请号：US12496549

    申请日：2009-07-01

    申请人： Leroy Hood ,  Andrew F. Siegel

    发明人： Leroy Hood ,  Andrew F. Siegel

    IPC分类号： G06F7/00

    CPC分类号： G01N33/6803 ,  G01N2800/60 ,  Y10S707/99943

    摘要： The invention provides a method of determining a comparative expression profile in an individual by comparing the expression levels of a sample of molecules in a population of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules, wherein expression levels within the health-associated reference expression region indicate a reference expression profile and wherein expression levels outside the health-associated reference expression region indicate a perturbed expression profile. The invention also provides methods of diagnosing a disease or a health state in an individual by comparing the expression level of a sample of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules.

    摘要翻译： 本发明提供了一种通过将来自个体的标本中的分子群体中的分子样品与分子样品的健康相关参考表达区域的分子样品的表达水平进行比较来确定个体中比较表达谱的方法，其中 健康相关参考表达区域内的表达水平指示参考表达谱，并且其中健康相关参考表达区域之外的表达水平表示扰动的表达谱。 本发明还提供了通过比较来自个体的样品中的分子样品与分子样品的健康相关参考表达区域的表达水平来诊断个体中疾病或健康状态的方法。

    Methods for high-throughput and quantitative proteome analysis
    45.
    发明授权
    Methods for high-throughput and quantitative proteome analysis 有权
    高通量和定量蛋白质组分析方法

    公开(公告)号：US07655433B2

    公开(公告)日：2010-02-02

    申请号：US10455246

    申请日：2003-06-04

    申请人： Rudolf H. Aebersold

    发明人： Rudolf H. Aebersold

    IPC分类号： C12Q1/37 ,  C12Q1/00 ,  C12Q1/34 ,  C12N9/48 ,  C12N9/76 ,  C12P19/30

    CPC分类号： G01N33/6848 ,  G01N33/60 ,  G01N33/6803 ,  G01N33/6818 ,  G01N33/6842

    摘要： The invention provides methods for identifying and quantifying polypeptides in a sample. The methods include the steps of labeling peptides in a polypeptide sample with an isotope tag; adding a plurality of peptide standards to the polypeptide sample, wherein the peptide standards are labeled with an isotopically distinct version of the isotope tag; resolving the labeled sample and standard peptides into a plurality of fractions; analyzing the resolved fractions using mass spectrometry; identifying an isotope-tagged sample peptide in an analyzed fraction; and determining the amount of the identified isotope-tagged sample peptide in the analyzed fraction by comparison to the amount of isotope tagged standard peptide in the same fraction.

    摘要翻译： 本发明提供了用于鉴定和定量样品中多肽的方法。 所述方法包括在具有同位素标签的多肽样品中标记肽的步骤; 向所述多肽样品中加入多个肽标准物，其中所述肽标准物用同位素不同型号的同位素标签标记; 将标记的样品和标准肽分解成多个级分; 使用质谱法分析分辨率; 鉴定分析部分中的同位素标记的样品肽; 并通过与相同级分中的同位素标记的标准肽的量进行比较来确定所分析的级分中鉴定的同位素标记的样品肽的量。

    Multiparameter analysis for predictive medicine
    47.
    发明授权
    Multiparameter analysis for predictive medicine 有权
    预测医学多参数分析

    公开(公告)号：US07567870B1

    公开(公告)日：2009-07-28

    申请号：US09724898

    申请日：2000-11-28

    申请人： Leroy Hood ,  Andrew F. Siegel

    发明人： Leroy Hood ,  Andrew F. Siegel

    IPC分类号： G06F7/00

    CPC分类号： G01N33/6803 ,  G01N2800/60 ,  Y10S707/99943

    摘要： The invention provides a method of determining a comparative expression profile in an individual by comparing the expression levels of a sample of molecules in a population of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules, wherein expression levels within the health-associated reference expression region indicate a reference expression profile and wherein expression levels outside the health-associated reference expression region indicate a perturbed expression profile. The invention also provides methods of diagnosing a disease or a health state in an individual by comparing the expression level of a sample of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules.

    摘要翻译： 本发明提供了一种通过将来自个体的标本中的分子群体中的分子样品与分子样品的健康相关参考表达区域的分子样品的表达水平进行比较来确定个体中比较表达谱的方法，其中 健康相关参考表达区域内的表达水平指示参考表达谱，并且其中健康相关参考表达区域之外的表达水平表示扰动的表达谱。 本发明还提供了通过比较来自个体的样品中的分子样品与分子样品的健康相关参考表达区域的表达水平来诊断个体中疾病或健康状态的方法。

    MULTIPARAMETER ANALYSIS FOR DRUG RESPONSE AND RELATED METHODS
    49.
    发明申请
    MULTIPARAMETER ANALYSIS FOR DRUG RESPONSE AND RELATED METHODS 审中-公开
    多药物反应分析及相关方法

    公开(公告)号：US20080131905A1

    公开(公告)日：2008-06-05

    申请号：US12019600

    申请日：2008-01-24

    申请人： Leroy E. Hood ,  Andrew F. Siegel

    发明人： Leroy E. Hood ,  Andrew F. Siegel

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12Q1/20

    CPC分类号： G16B40/00 ,  G16B25/00

    摘要： The invention provides a method of determining a comparative expression profile in an individual by comparing the expression levels of a sample of molecules in a population of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules, wherein expression levels within the health-associated reference expression region indicate a reference expression profile and wherein expression levels outside the health-associated reference expression region indicate a perturbed expression profile. The invention also provides methods of diagnosing a disease or a health state in an individual by comparing the expression level of a sample of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules. The invention additionally provides a method of classifying a population by drug responsiveness.

    摘要翻译： 本发明提供了一种通过将来自个体的标本中的分子群体中的分子样品与分子样品的健康相关参考表达区域的分子样品的表达水平进行比较来确定个体中比较表达谱的方法，其中 健康相关参考表达区域内的表达水平指示参考表达谱，并且其中健康相关参考表达区域之外的表达水平表示扰动的表达谱。 本发明还提供了通过比较来自个体的样品中的分子样品与分子样品的健康相关参考表达区域的表达水平来诊断个体中疾病或健康状态的方法。 本发明另外提供了通过药物反应性对群体进行分类的方法。

    Compositions and methods for drop boundary detection and radiation beam alignment
    50.
    发明授权
    Compositions and methods for drop boundary detection and radiation beam alignment 有权
    液滴边界检测和辐射束对准的组成和方法

    公开(公告)号：US07362424B2

    公开(公告)日：2008-04-22

    申请号：US10132659

    申请日：2002-04-24

    申请人： Gerrit J. van den Engh ,  Peter I. Nelson

    发明人： Gerrit J. van den Engh ,  Peter I. Nelson

    IPC分类号： G01B9/02

    CPC分类号： G01N15/1404 ,  G01N15/1456 ,  G01N2015/1406 ,  G01N2015/149

    摘要： The invention provides an apparatus and method for determining the position of a radiation beam. The apparatus includes (a) a first reflective surface and a second reflective surface, the reflective surfaces being placed to form the reflective exterior of a wedge; (b) a first detector placed to detect radiation reflected from the first reflective surface, and (c) a second detector placed to detect radiation reflected from the second reflective surface. The method includes the steps of (a) directing a radiation beam to the reflective exterior of a wedge formed by a first reflective surface and a second reflective surface; (b) selectively detecting radiation reflected from the first reflective surface; (c) selectively detecting radiation reflected from the second reflective surface; and (d) determining the position of the radiation beam based on the difference in the amount of radiation detected from each surface.

    摘要翻译： 本发明提供了一种用于确定辐射束位置的装置和方法。 该装置包括（a）第一反射表面和第二反射表面，反射表面被放置以形成楔形物的反射外部; （b）第一检测器，被设置为检测从第一反射表面反射的辐射，以及（c）第二检测器，被设置成检测从第二反射表面反射的辐射。 该方法包括以下步骤：（a）将辐射束引导到由第一反射表面和第二反射表面形成的楔的反射外部; （b）选择性地检测从第一反射表面反射的辐射; （c）选择性地检测从第二反射表面反射的辐射; 和（d）基于从每个表面检测到的辐射量的差异来确定辐射束的位置。