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公开(公告)号：US07794947B2
公开(公告)日：2010-09-14
申请号：US10888084
申请日：2004-07-09
申请人： Rudolf H. Aebersold , Hui Zhang
发明人： Rudolf H. Aebersold , Hui Zhang
IPC分类号： G01N33/53
CPC分类号： G01N33/6848 , C12Q1/37 , G01N33/6845
摘要： The invention provides methods of detecting polypeptides in a sample. The method can include the steps of cleaving polypeptides in a test sample to generate peptides; adding a predetermined amount of isotopically labeled peptide standards to the cleaved test sample, wherein the peptide standards correspond to peptides cleaved with the same reagent used to cleave the test sample; contacting the cleaved test sample containing peptide standards with an array of immobilized binding agents specific for the peptide standards; washing the array to remove unbound peptides, thereby retaining affinity captured sample peptides and standard peptides; analyzing the affinity captured peptides using mass spectrometry; and determining the presence of bound test peptides and standard peptides. The method can further include the step of quantifying the amount of the test peptides by comparing the ratio of test peptide to corresponding standard peptide.
摘要翻译： 本发明提供了检测样品中多肽的方法。 该方法可以包括在测试样品中切割多肽以产生肽的步骤; 向切割的测试样品中加入预定量的同位素标记的肽标准物，其中肽标准物对应于用用于切割测试样品的相同试剂切割的肽; 使含有肽标准物的切割的测试样品与肽标准物特异性的固定化结合剂阵列接触; 洗涤阵列以除去未结合的肽，从而保留亲和力捕获的样品肽和标准肽; 使用质谱分析亲和力捕获的肽; 并确定结合的测试肽和标准肽的存在。 该方法还可以包括通过比较测试肽与相应的标准肽的比例来量化测试肽的量的步骤。
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公开(公告)号：US20100279382A1
公开(公告)日：2010-11-04
申请号：US12723574
申请日：2010-03-12
申请人： Rudolf H. Aebersold , Hui Zhang
发明人： Rudolf H. Aebersold , Hui Zhang
CPC分类号： G01N33/6848 , G01N33/6842 , G01N2400/00 , G01N2458/15
摘要： The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.
摘要翻译： 本发明提供用于从人血清或血浆中鉴定和/或定量糖多肽的组合物和方法。 组合物和方法包括多个含有针对人血清/血浆蛋白质测定的糖基化位点的标准肽。
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公开(公告)号：US07183118B2
公开(公告)日：2007-02-27
申请号：US10454375
申请日：2003-06-03
申请人： Rudolf H. Aebersold , Hui Zhang
发明人： Rudolf H. Aebersold , Hui Zhang
IPC分类号： G01N24/00
CPC分类号： G01N33/6848 , C12Q1/34 , C12Q1/37 , G01N33/68 , G01N33/6803 , G01N33/6842 , G01N33/6851 , G01N2400/00 , G01N2458/15 , Y10T436/24 , Y10T436/25125
摘要： The invention provides a method for identifying and quantifying polyglycopeptides in a sample. The method can include the steps of immobilizing glycopolypeptides to a solid support; cleaving the immobilized glycopolypeptides, thereby releasing non-glycosylated peptides and retaining immobilized glycopeptides; releasing the glycopeptides from the solid support; and analyzing the released glycopeptides. The method can further include the step of identifying one or more glycopeptides, for example, using mass spectrometry.
摘要翻译： 本发明提供了一种用于鉴定和定量样品中的聚糖肽的方法。 该方法可以包括将糖多肽固定在固体支持物上的步骤; 切割固定化的糖多肽，从而释放非糖基化肽并保留固定的糖肽; 从固体支持物释放糖肽; 并分析释放的糖肽。 该方法还可以包括鉴定一种或多种糖肽的步骤，例如使用质谱法。
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4.发明授权公开(公告)号：US08222208B2
公开(公告)日：2012-07-17
申请号：US13007393
申请日：2011-01-14
CPC分类号： C07K16/28
摘要： The invention provides methods for preparing a peptide as an immunogen to generate an antibody specific for the deglycosylated form of a glycopolypeptide by preparing a peptide that includes an N-linked glycosylation site of said glycopolypeptide and identifying a peptide sequence that includes said glycosylated site and preparing said peptide wherein N of said site is converted to D.
摘要翻译： 本发明提供了制备肽作为免疫原的方法，通过制备包含所述糖多肽的N-连接的糖基化位点的肽并鉴定包含所述糖基化位点的肽序列并制备了包含所述糖基化位点的肽序列，从而产生对糖多糖的去糖基化形式特异性的抗体 所述肽，其中所述位点的N转化为D.
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5.发明授权公开(公告)号：US07879799B2
公开(公告)日：2011-02-01
申请号：US11891487
申请日：2007-08-09
CPC分类号： C07K16/28
摘要： The invention provides methods for generating an antibody specific for the deglycosylated form of a glycopolypeptide using a peptide corresponding to an N-linked glycosylation site of a glycopolypeptide. The invention additionally provides methods for generating an antibody specific for a glycopolypeptide using a peptide corresponding to amino acids adjacent to an authentic N-linked glycosylation site.
摘要翻译： 本发明提供使用对应于糖多肽的N-连接糖基化位点的肽产生对糖多糖形式的去糖基化形式特异的抗体的方法。 本发明另外提供了使用对应于与真实N-连接的糖基化位点相邻的氨基酸的肽来产生对糖多肽特异性的抗体的方法。
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6.发明申请CHEMICAL REAGENTS AND METHODS FOR DETECTION AND QUANTIFICATION OF PROTEINS IN COMPLEX MIXTURES 审中-公开化学试剂和复合混合物中蛋白质检测和定量方法公开(公告)号：US20090111195A1
公开(公告)日：2009-04-30
申请号：US12332261
申请日：2008-12-10
IPC分类号： G01N33/53 , C07D271/12 , C07D495/04 , C07K5/08 , C13K5/00 , C07K1/14 , C07K1/113 , C07K1/00
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译： 本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。 本发明还提供了使用本发明试剂的方法。 该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。 本发明另外提供了分离，鉴定和定量样品中的多肽的方法。 本发明还提供使用本发明的试剂诊断疾病的方法。
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7.发明申请ANDROGEN-REGULATED GENES AND USES FOR DIAGNOSIS, PROGNOSIS AND TREATMENT OF PROSTATE NEOPLASTIC CONDITIONS 有权用于诊断，预防和治疗前列腺增生症状的原发性基因和用途公开(公告)号：US20100196883A1
公开(公告)日：2010-08-05
申请号：US12372644
申请日：2009-02-17
IPC分类号： C12Q1/68 , G01N33/567
CPC分类号： G01N33/57434 , G01N2333/726 , G01N2500/00
摘要： The invention provides a method for diagnosing or predicting susceptibility to a prostate neoplastic condition in an individual. The method involves (a) determining a level of RDC1 in a sample from the individual, and (b) comparing the level of RDC1 in the sample to a reference level of RDC1, wherein a level of RDC1 in the sample 2-fold or more higher than the reference level indicates the presence of, or susceptibility to, a prostate neoplastic condition in the individual.
摘要翻译： 本发明提供了用于诊断或预测个体对前列腺肿瘤病症的易感性的方法。 该方法包括（a）确定来自个体的样品中RDC1的水平，和（b）将样品中RDC1的水平与RDC1的参考水平进行比较，其中样品中RDC1的水平为2倍以上 高于参考水平表明个体中前列腺肿瘤状况的存在或易感性。
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8.发明授权Chemical reagents and methods for detection and quantification of proteins in complex mixtures 有权用于复杂混合物中蛋白质的检测和定量的化学试剂和方法公开(公告)号：US07473535B2
公开(公告)日：2009-01-06
申请号：US10225447
申请日：2002-08-20
CPC分类号： G01N33/585 , G01N33/6803 , G01N33/6809
摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.
摘要翻译： 本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。 本发明还提供了使用本发明试剂的方法。 该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。 本发明另外提供了分离，鉴定和定量样品中的多肽的方法。 本发明还提供使用本发明的试剂诊断疾病的方法。
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公开(公告)号：US07045296B2
公开(公告)日：2006-05-16
申请号：US09851058
申请日：2001-05-08
申请人： Kenneth C. Parker , Timothy K. Nadler , George J. Vella , Yulin Huang , Rudolf H. Aebersold , Marcus B. Smolka
发明人： Kenneth C. Parker , Timothy K. Nadler , George J. Vella , Yulin Huang , Rudolf H. Aebersold , Marcus B. Smolka
CPC分类号： G01N33/6803 , Y10S435/964 , Y10T436/24 , Y10T436/25125
摘要： Methods using gel electrophoresis and mass spectrometry for the rapid, quantitative analysis of proteins or protein function in mixtures of proteins derived from two or more samples in one unit operation are disclosed. In one embodiment the method includes (a) preparing an extract of proteins from each of at least two different samples; (b) providing a set of substantially chemically identical and differentially isotopically labeled protein reagents; (c) reacting the extract of proteins from different samples of step (a) with a different isotopically labeled reagent from the set of step (b) to provide two or more sets of isotopically differentially labeled proteins; (d) mixing each of the two or more sets of isotopically labeled proteins to form a single mixture of isotopically differentially labeled proteins; (e) electrophoresing the mixture of step (d) by an electrophoresing method capable of separating proteins within the mixture; (f) digesting at least a portion of one or more separated proteins of step (e) and (g) detecting the difference in the expression levels of the proteins in the two samples by mass spectrometry based on one or more peptides in the sample of labeled peptides. The analytical method can be used for qualitative and particularly for quantitative analysis of global protein expression profiles in cells and tissues, i.e. the quantitative analysis of proteomes.
摘要翻译： 公开了使用凝胶电泳和质谱法在一个单元操作中从两个或更多个样品衍生的蛋白质的蛋白质或蛋白质功能的快速，定量分析的方法。 在一个实施方案中，该方法包括（a）从至少两种不同样品中的每一种制备蛋白质提取物; （b）提供一组基本上化学相同和差异同位素标记的蛋白质试剂; （c）使来自步骤（a）的不同样品的蛋白质提取物与来自步骤（b）的组的不同的同位素标记的试剂反应，以提供两组或更多组同位素差异标记的蛋白质; （d）混合两组或更多组同位素标记的蛋白质以形成同位素差异标记的蛋白质的单一混合物; （e）通过能够分离混合物内的蛋白质的电泳方法电泳步骤（d）的混合物; （f）消化步骤（e）中的一种或多种分离的蛋白质的至少一部分，和（g）基于样品中的一种或多种肽通过质谱法检测两种样品中的蛋白质的表达水平的差异 标记肽。 分析方法可用于定性，特别是定量分析细胞和组织中的全球蛋白表达谱，即蛋白质组的定量分析。
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公开(公告)号：US5856082A
公开(公告)日：1999-01-05
申请号：US299290
申请日：1994-08-31
CPC分类号： C12Q1/00
摘要： The present invention provides analytical devices for the characterization of the primary structure of proteins and peptides, comprising a microenzyme reactor, a separation device, an interface between the microenzyme reactor and the separation device, a mass spectrometer, and an interface between the separation device and the mass spectrometer. Also provided are methods for characterizing a protein or peptide utilizing such devices.
摘要翻译： 本发明提供了用于表征蛋白质和肽的一级结构的分析装置，包括微酶反应器，分离装置，微酶反应器和分离装置之间的界面，质谱仪以及分离装置和 质谱仪。 还提供了利用这些装置表征蛋白质或肽的方法。
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