PREPARATION METHOD AND APPLICATION OF VISCOSITY FLUORESCENT PROBE

    公开(公告)号:US20240302280A1

    公开(公告)日:2024-09-12

    申请号:US18589519

    申请日:2024-02-28

    摘要: The present invention belongs to the field of organic small molecule fluorescent probes, and in particular to a preparation method and application of a viscosity fluorescent probe. A viscosity fluorescent probe TzAr-Nap-OH based on 1,3,5 triazine dye, which shows very strong sensitivity to viscosity, has little effect on fluorescence intensity over a wide pH range and good cytocompatibility, and responds well to viscosity. In cell imaging experiments, the fluorescent probe has low cytotoxicity and may accurately locate cells, may be applied to detect cell viscosity of complex organisms, and the synthesis steps of the probe are simple with a wide range of raw material sources, which provides an available tool for studying the important role that cell viscosity plays in complex physiological processes of organisms. Therefore, the cell viscosity fluorescent probe with high sensitivity and low interference and toxicity has broad application prospects in the field of biomolecular detection.