公开(公告)号：US20040153249A1

公开(公告)日：2004-08-05

申请号：US10635241

申请日：2003-08-05

Applicant: The Johns Hopkins University

Inventor： Zhen Zhang ,  Daniel Wanyui Chan

IPC: G06F015/18 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N33/6803 ,  C12Q1/6876 ,  G16B20/00 ,  G16B25/00 ,  G16B40/00 ,  H01J49/00

Abstract: The invention provides a method, system and software to screen for, identify and validate biomarkers that are predictive of a biological state, such as a cell state and/or patient status.

Abstract translation: 本发明提供了一种方法，系统和软件，用于筛选，鉴定和验证预测生物状态的细胞状态和/或患者状态的生物标志物。

公开(公告)号：US20040152089A1

公开(公告)日：2004-08-05

申请号：US10471113

申请日：2004-03-12

Inventor： Uwe Kraemer ,  Heinz-Michael Hein ,  Dietmar Volz

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: A61B5/1455 ,  A61B5/14532 ,  A61B2562/0242 ,  Y10T436/144444

Abstract: A method for the selective determination of a light transport parameter which is characteristic for the light scattering in a biological matrix (5), in particular for the purpose of the non-invasive determination of the glucose concentration in the biological matrix. It comprises a plurality of detection measurements, in which light is irradiated as primary light into the biological matrix and an intensity value of secondary light emerging at a detection site, located in different measuring distances from the irradiation site, is measured. In an evaluation step, the light transport parameter is derived, by means of an evaluation algorithm, from the measured intensity values. For the selective determination of the scattering coefficient, the evaluation algorithm includes a step in which a time derivative value nulltI(r) describing the change of the measurement intensity value versus time is calculated from intensity measurement values obtained at a minimum of two different points of time.

Abstract translation: 一种用于选择性地确定生物基质（5）中光散射特征的光传输参数的方法，特别是用于非侵入式确定生物基质中的葡萄糖浓度的目的。 它包括多个检测测量，其中将光作为主要光照射到生物基质中，并且测量出现在与照射部位不同测量距离的检测部位处的二次光的强度值。 在评估步骤中，通过评估算法从测量的强度值导出光传输参数。 为了选择性地确定散射系数，评估算法包括一个步骤，其中描述测量强度值对时间的变化的时间导数值DeltatI（r）是从在两个不同点的最小两点获得的强度测量值计算的 时间。

公开(公告)号：US20040147033A1

公开(公告)日：2004-07-29

申请号：US10742617

申请日：2003-12-19

Inventor： Zachary Shriver ,  Ganesh Venkataraman ,  Ram Sasisekharan ,  Mallikarjun Sundaram

IPC: G01N033/48

CPC classification number: G01N33/574 ,  G01N33/57473 ,  G01N33/57476 ,  G01N2400/10 ,  G16B50/00

Abstract: The present invention provides ultra-sensitive methods for detecting changes in glycosylation that are correlated with pre-cancerous or early cancerous states. Because the chance of complete recovery is increased with earlier detection of cancer, the present invention provides therapeutically useful methods of early detection, diagnosis, staging and prognostication.

Abstract translation: 本发明提供了用于检测与癌前或早期癌症状态相关的糖基化变化的超灵敏方法。 因为随着癌症的早期检测，完全恢复的机会增加，本发明提供治疗有用的早期检测，诊断，分期和预后的方法。

公开(公告)号：US20040146909A1

公开(公告)日：2004-07-29

申请号：US10714489

申请日：2003-11-14

Inventor： Hau H. Duong ,  Stephen D. O'Connor ,  Robert H. Terbrueggen ,  Jon Faiz Kayyem ,  Gary T. Olsen ,  Daniel A. Litvack ,  Javier Gonzalez

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G01N27/416 ,  B82Y15/00 ,  B82Y30/00 ,  G01N27/3277 ,  G01N33/5438

Abstract: The invention relates to the use of signal processing methods in order to acheive higher signal to noise ratios, to increase the detection limits of target analytes. These techniques include the monitoring of the output signal at higher harmonic frequencies.

Abstract translation: 本发明涉及使用信号处理方法以达到更高的信噪比，从而增加目标分析物的检测限。 这些技术包括在较高谐波频率下监测输出信号。

公开(公告)号：US20040142403A1

公开(公告)日：2004-07-22

申请号：US10702236

申请日：2003-11-05

Inventor： Donald Hetzel ,  Stephen L. Monfre ,  Kevin H. Hazen ,  Timothy L. Ruchti ,  Thomas B. Blank ,  Linda Hockersmith ,  Andrew Cone

IPC: C12Q001/54 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: C12Q1/54 ,  A61B5/14532 ,  A61B5/7264 ,  G01N33/66 ,  G01N2800/042 ,  G16H50/20 ,  G16H50/70

Abstract: A method of screening for disorders of glucose metabolism such as impaired glucose tolerance and diabetes allows prevention, or early detection and treatment of diabetic complications such as cardiovascular disease, retinopathy, and other disorders of the major organs and systems. A mathematical algorithm evaluates the shape of a subject's glucose profile and classifies the profile into one of several predefined clusters, each cluster corresponding either to a normal condition or one of several abnormal conditions. The series of blood glucose values making up the glucose tolerance curve may be measured using any glucose analyzer including: invasive, minimally invasive and noninvasive types. The method is executed on a processing device programmed to perform the steps of the method. Depending on the outcome of the screening, a subject may be provided with additional information concerning their condition and/or counseled to consult further with their health care provider.

公开(公告)号：US20040142370A1

公开(公告)日：2004-07-22

申请号：US10750271

申请日：2004-01-02

Applicant: Bayer Healthcare LLC

Inventor： Andrew J. Dosmann ,  Frank W. Wogoman

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50 ,  C12M001/34

CPC classification number: A61B5/1455 ,  A61B5/14532 ,  A61B2562/0238 ,  B01L3/508 ,  B01L2300/0654 ,  B01L2300/0672 ,  G01N21/0303

Abstract: An optical waveguiding optical format enables consistent optical analysis of small sample volumes. The optical format is comprised of an illumination light guide, a read window upon which a sample is placed, a sample collection needle or capillary, and a detection guide. Light redirecting facets are provided within the format itself such that the format serves as a unitary component for accepting light, directing light through a sample, and emitting light for detection.

Abstract translation: 光学波导光学格式使得能够对小样本体积进行一致的光学分析。 光学格式包括照明光导，放置样品的读取窗口，样本采集针或毛细管以及检测引导件。 光重定向小面在格式本身内提供，使得该格式用作接受光，将光引导通过样本并发射光用于检测的整体部件。

公开(公告)号：US20040138820A1

公开(公告)日：2004-07-15

申请号：US10340108

申请日：2003-01-10

Inventor： Deborah A. Morris ,  W. Calvin Morris

IPC: G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: G06F19/3475

Abstract: A method for controlling body weight. The method is based on assigning a Amples value to food items based on the mathematical calculation: Amples valuenull(E/P)/S, where E is the commonly expressed kilocalorie content of the food item; P is the propensity of the food item to elicit Passive Over Consumption; and S is the ability of the food item to create the feeling of satisfaction. Values are assigned to all food items based on observation, experience, case studies and education. Values can be changed for each individual based on their own experiences. Amples are tabulated in any easy to read reference form. The serving sizes upon which the calculations are based on readily estimable amounts using everyday spoons, bowls, plates, and baking dishes with values assigned based on an across-the-board average of all food items.

Abstract translation: 一种控制体重的方法。 该方法基于基于数学计算为食物分配Amples值：Amples值=（E / P）/ S，其中E是食品的通常表达的kilocalorie含量; P是食物物品引起被动消费的倾向; 而S则是食物产生满足感的能力。 根据观察，经验，案例研究和教育，将价值分配给所有食品。 可以根据自己的经验为每个人改变价值。 Amples以任何易于阅读的参考表格列出。 使用日常勺子，碗，盘子和烘焙食品，根据所有食品的全面平均值分配的价值，计算的基础是容易估计的量。

公开(公告)号：US20040137475A1

公开(公告)日：2004-07-15

申请号：US10683753

申请日：2003-10-13

Inventor： Richard P. Haugland ,  Stephen T. Yue

IPC: C12Q001/68 ,  G01N033/53 ,  G01N033/48 ,  C07D421/02 ,  C07D417/02 ,  C07D413/02 ,  C07D43/02

CPC classification number: C09B23/02 ,  C12Q1/6816 ,  C12Q1/6876 ,  G01N1/30 ,  G01N27/44726 ,  G01N33/52 ,  G01N33/5308 ,  G01N33/531 ,  G01N33/582 ,  G01N33/68 ,  G01N2001/302 ,  Y10S436/80 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/107

Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.

Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料，包括由阳离子侧链取代的花青染料，单体和二聚花青染料，化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时，受试染料实际上是非荧光的，但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时，它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用，包括溶液，电泳凝胶，细胞和微生物。

公开(公告)号：US20040137432A1

公开(公告)日：2004-07-15

申请号：US09810670

申请日：2001-03-19

Applicant: Akiko ITAI

Inventor： Akiko Itai ,  Nobuo Tomioka

IPC: C12Q001/68 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: C07B61/00 ,  G16C20/40 ,  G16C20/50 ,  G16C20/90

Abstract: A method for selecting lead-candidate compounds capable of binding to a receptor biopolymer from a database containing information about atomic types and mode of covalent bonds of compounds by using a computer, comprising a step of selecting candidate compounds from compounds stored in the database based on quantitative, two-dimensional and/or three-dimensional information of one or more query molecules capable of binding to the biopolymer. The query molecules can be obtained by an automatic structure construction method, for example. The lead-candidate compounds capable of binding to the biopolymer can be retrieved rapidly by using an ordinary personal computer or workstation without requiring huge calculation.

Abstract translation: 一种选择能够通过使用计算机从包含化合物的原子类型和共价键的模式的数据库的数据库中选择能够结合受体生物聚合物的候选铅化合物的方法，包括从基于数据库中存储的化合物中选择候选化合物的步骤 能够结合生物聚合物的一个或多个查询分子的定量，二维和/或三维信息。 查询分子可以通过例如自动结构构造方法获得。 可以通过使用普通个人计算机或工作站快速检索能够结合生物聚合物的首选候选化合物，而不需要大量计算。

公开(公告)号：US20040133357A1

公开(公告)日：2004-07-08

申请号：US10723434

申请日：2003-11-26

Applicant: Abmaxis, Inc.

Inventor： Pingyu Zhong ,  Peizhi Luo ,  Kevin C. Wang ,  Mark Hsieh ,  Yan Li

IPC: C07K016/22 ,  G01N033/53 ,  G06F019/00 ,  G01N033/48 ,  G01N033/50

CPC classification number: C07K16/00 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/007 ,  B01J2219/00725 ,  C07K16/22 ,  C07K2299/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/92 ,  C07K2319/00 ,  C40B40/10 ,  Y02A90/26

Abstract: Methods are provided for designing and selecting antibodies against human antigens with high affinity and specificity in silico and in vitro. In some particular embodiments, methods are provided for designing and selecting humanized or fully human antibodies against vascular endothelial growth factor (VEGF) with high affinity and specificity. In another aspect of the invention, monoclonal antibodies against VEGF are provided. In particular, humanized or human anti-VEGF monoclonal antibodies are provided with ability to bind to human VEGF with high affinity, inhibit VEGF-induced proliferation of endothelial cells in vitro and inhibit VEGF-induced angiogenesis in vivo. These antibodies and their derivative can be used in a wide variety of applications such as diagnosis, prevention, and treatment of diseases such as cancer, AMD, diabetic retinopathy, and other diseases derived from pathological angiogenesis.

Abstract translation: 提供了用于在计算机和体外以高亲和力和特异性设计和选择针对人抗原的抗体的方法。 在一些具体实施方案中，提供了用于以高亲和力和特异性设计和选择针对血管内皮生长因子（VEGF）的人源化或完全人抗体的方法。 在本发明的另一方面，提供了针对VEGF的单克隆抗体。 特别地，人源化或人抗VEGF单克隆抗体具有以高亲和力结合人VEGF的能力，在体外抑制VEGF诱导的内皮细胞增殖并抑制体内VEGF诱导的血管生成。 这些抗体及其衍生物可用于广泛的应用，例如诊断，预防和治疗诸如癌症，AMD，糖尿病性视网膜病和其它源于病理血管发生的疾病。