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公开(公告)号：US20190048401A1
公开(公告)日：2019-02-14
申请号：US16167893
申请日：2018-10-23
发明人： Elazar Rabbani , James J. Donegan , Dakai Liu
IPC分类号： C12Q1/6818 , C12Q1/70 , C12Q1/6876 , C12Q1/6853 , C12Q1/6844
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided. Paneling and multiplex analyses of more than one nucleic acid analyte using one sample are also provided.
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公开(公告)号：US10197581B2
公开(公告)日：2019-02-05
申请号：US15254017
申请日：2016-09-01
摘要： The disclosure relates to methods for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid sample. The disclosure further relates to kits for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid.
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公开(公告)号：US20190002491A1
公开(公告)日：2019-01-03
申请号：US16127950
申请日：2018-09-11
发明人： Zaiguo Li , Praveen Pande
摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
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公开(公告)号：US20180223328A1
公开(公告)日：2018-08-09
申请号：US15944336
申请日：2018-04-03
IPC分类号： C12Q1/34 , C07D493/10 , C07D311/16
CPC分类号： C12Q1/34 , C07D311/16 , C07D493/10 , C12Y305/01098 , G01N2333/98
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
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公开(公告)号：US09963734B2
公开(公告)日：2018-05-08
申请号：US15464966
申请日：2017-03-21
IPC分类号： C07D311/96 , C12Q1/34 , C07D493/10 , C07D311/16
CPC分类号： C12Q1/34 , C07D311/16 , C07D493/10 , C12Y305/01098 , G01N2333/98
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
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公开(公告)号：US20170198333A1
公开(公告)日：2017-07-13
申请号：US15464966
申请日：2017-03-21
IPC分类号： C12Q1/34 , C07D311/16 , C07D493/10
CPC分类号： C12Q1/34 , C07D311/16 , C07D493/10 , C12Y305/01098 , G01N2333/98
摘要： Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ε-amino of a lysine.
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公开(公告)号：US20160370387A1
公开(公告)日：2016-12-22
申请号：US15254017
申请日：2016-09-01
IPC分类号： G01N33/82
CPC分类号： G01N33/82 , G01N33/5308
摘要： The disclosure relates to methods for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid sample. The disclosure further relates to kits for measuring levels of 25(OH)vitamin D (25OHD) in a mammalian fluid.
摘要翻译： 本公开涉及用于测量哺乳动物流体样品中25（OH）维生素D（25OHD）水平的方法。 本公开还涉及用于测量哺乳动物流体中25（OH）维生素D（25OHD）水平的试剂盒。
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公开(公告)号：US20160311846A1
公开(公告)日：2016-10-27
申请号：US15205438
申请日：2016-07-08
发明人： Zaiguo Li , Praveen Pande
摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.
摘要翻译： 一种方法实施方案包括使荧光染料化合物的反应性基团Z与靶分子接触，使得反应性基团Z与靶分子反应以在基团和靶分子之间形成共价键。 另一种方法实施方案包括使进一步包含结合对的第一个成员的荧光染料化合物与包含结合对的第二个成员的靶分子接触。 还提供了用荧光染料化合物标记的靶分子。
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公开(公告)号：US20140370028A1
公开(公告)日：2014-12-18
申请号：US14080644
申请日：2013-11-14
发明人： GERARD NUOVO
CPC分类号： C12Q1/705 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , G01N33/6869
摘要： This disclosure relates to methods of diagnosing a viral disease such as idiopathic pulmonary fibrosis, Castleman's disease, a lymphoma, a thymoma or a sarcoma in a patient by identifying one or more virus-specific elements such as a nucleic acid or a viral protein or a patient antibody to a virus-specific element, as well as to kits for diagnosing the viral disease in a patient. The disclosure further relates to methods of monitoring disease progression and/or the efficacy of therapy by measuring the levels of a virus-specific element in a sample from a patient. In addition, the disclosure relates to methods of identifying therapeutic agents that show efficacy in reducing levels of virus-specific agents in vitro. The disclosure further relates to methods of treating idiopathic pulmonary fibrosis, a lymphoproliferative disease and cancer, as well as to methods of preventing viral infection, including Herpesvirus saimiri infection.
摘要翻译： 本公开涉及通过鉴定一种或多种病毒特异性元件如核酸或病毒蛋白或病毒蛋白质来诊断患者中病毒性疾病如特发性肺纤维化，卡斯尔曼氏病，淋巴瘤，胸腺瘤或肉瘤的方法， 患者对病毒特异性元件的抗体，以及用于诊断患者病毒性疾病的试剂盒。 本公开还涉及通过测量来自患者的样品中病毒特异性元件的水平来监测疾病进展和/或治疗功效的方法。 此外，本公开涉及鉴定在体外降低病毒特异性试剂水平的效力的治疗剂的鉴定方法。 本发明进一步涉及治疗特发性肺纤维化，淋巴组织增生性疾病和癌症的方法，以及预防病毒感染的方法，包括疱疹病毒西米尔感染。
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公开(公告)号：US20130217059A1
公开(公告)日：2013-08-22
申请号：US13733324
申请日：2013-01-03
CPC分类号： C09B23/164 , C09B23/0066 , C09B23/0075 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146 , C12Q1/68 , G01N33/583
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
摘要翻译： 本发明提供可用于检测或定量所需靶分子（例如蛋白质，核酸和细胞器器）的染料，活性染料和标记试剂。 提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。 还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。 已经对本发明的新型染料进行了改进以提供有益的性质。
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