公开(公告)号：US06534269B2

公开(公告)日：2003-03-18

申请号：US09789556

申请日：2001-02-22

申请人： Qiang Liu ,  Steve S. Sommer

发明人： Qiang Liu ,  Steve S. Sommer

IPC分类号： C12Q168

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2565/301 ,  C12Q2525/186 ,  C12Q2535/125

摘要： A novel method of pyrophosphorolysis activated polymerization (PAP) has been developed. In PAP, pyrophosphorolysis and polymerization by DNA polymerase are coupled serially for each amplification by using an activatable oligonucleotide P* that has a non-extendable 3′-deoxynucleotide at its 3′ terminus. PAP can be applied for exponential amplification or for linear amplification. PAP can be applied to amplification of a rare allele in admixture with one or more wild type alleles by using an activatable oligonucleotide P* that is an exact match at its 3′ end for the rare allele but has a mismatch at or near its 3′ terminus for the wild type allele. PAP is inhibited by a mismatch in the 3′ specific subsequence as far as 16 nucleotides away from the 3′ terminus. PAP can greatly increase the specificity of detection of an extremely rare mutant allele in the presence of the wild type allele. Specificity results from both pyrophosphorolysis and polymerization since significant nonspecific amplification requires the combination of mismatch pyrophosphorolysis and misincorporation by the DNA polymerase, an extremely rare event. Using genetically engineered DNA polymerases greatly improves the efficiency of PAP.

公开(公告)号：US20200150047A1

公开(公告)日：2020-05-14

申请号：US16658029

申请日：2019-10-19

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： G01N21/76 ,  C12Q1/6844

摘要： A new fluorescence detection method called pyrophosphorolysis activated fluorescence was developed to measure PAP amplification of nucleic acid. A fluorophore-quencher dual-attached blocked primer was used for PAP which has a fluorophore attached to a nucleotide in the internal region or at the 5′ end and a quencher attached to a blocked nucleotide at the 3′ end. Multiple fluorophore-quencher dual-labeled blocked primers were also used for multiplex PAP, which are attached with different fluorophores to distinguish multiple templates in a reaction.

公开(公告)号：US20190271035A1

公开(公告)日：2019-09-05

申请号：US16408442

申请日：2019-05-09

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Tony Zhou Lee ,  Qiang Liu

IPC分类号： C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6858

摘要： Multiplex pyrophosphorolysis activated polymerization uses multiple pairs of blocked primers to amplify multiple potential templates in a single reaction, including those almost-sequence-identical templates located in one locus. To identify and differentiate the multiple amplified products, individual molecules are sequenced in parallel. Thus multiplex PAP amplification is combined with parallel sequencing for ultrahigh-sensitive, ultrahigh-selective and ultrahigh-throughput detection of early cancer.

公开(公告)号：US20180073067A1

公开(公告)日：2018-03-15

申请号：US15825114

申请日：2017-11-29

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6848 ,  C12Q1/6844 ,  C12Q2527/125

摘要： The invention provides a method for lyophilizing integrated composition of pyrophosphorolysis activated polymerization (PAP) in an aqueous solution. It also provides lyophilized integrated PAP composition. Except for nucleic acid template, the integrated composition contains all components. For manipulation, simply add nucleic acid template in an aqueous solution to start amplification. In addition to the easy manipulation, the lyophilized integrated composition can be stored for prolonged period at ambiguous temperature.

公开(公告)号：US09470205B2

公开(公告)日：2016-10-18

申请号：US13802590

申请日：2013-03-13

申请人： Qiang Liu ,  Myles L. Baker

发明人： Qiang Liu ,  Myles L. Baker

IPC分类号： F03D1/06 ,  F03D1/00 ,  B29C70/52 ,  B29L31/08 ,  B29C65/48 ,  B29C65/00

CPC分类号： F03D1/0675 ,  B29C65/48 ,  B29C66/1248 ,  B29C66/43 ,  B29C66/721 ,  B29C70/52 ,  B29L2031/085 ,  F03D13/10 ,  F05B2240/302 ,  Y02E10/721 ,  Y02E10/728 ,  Y02P70/523 ,  Y10T29/49337

摘要： Wind turbine blades with layered, multi-component spars, and associated systems and methods are disclosed. A wind turbine blade system in accordance with a particular embodiment includes a first blade segment having a first spar element that includes first planks having a first thickness and a first plank composition, and a second blade segment having a second spar element that includes second planks having a second thickness and a second plank composition different than the first plank composition. The second blade segment is joined to the first blade segment at a joint, and, in particular embodiments, an overall product of thickness and elastic modulus of the first planks is approximately equal to an overall product of thickness and elastic modulus for the second planks.

摘要翻译： 公开了具有分层，多组分翼梁以及相关系统和方法的风力涡轮机叶片。 根据特定实施例的风力涡轮机叶片系统包括具有第一翼梁元件的第一叶片段，所述第一翼梁元件包括具有第一厚度的第一木板和第一木板组合物，以及具有第二翼梁元件的第二叶片段，所述第二翼梁元件包括第二木板， 第二厚度和与第一板组合物不同的第二板组合物。 第二刀片段在接头处连接到第一刀片段，并且在具体实施例中，第一板的厚度和弹性模量的总体乘积近似等于第二板的厚度和弹性模量的总积。

公开(公告)号：US09178826B2

公开(公告)日：2015-11-03

申请号：US13820798

申请日：2011-08-24

申请人： Caixia Chi ,  Fang Liu ,  Qiang Liu ,  Dong Yan ,  Bin Wang

发明人： Caixia Chi ,  Fang Liu ,  Qiang Liu ,  Dong Yan ,  Bin Wang

IPC分类号： H04L12/26 ,  H04L12/803 ,  H04L12/851 ,  H04L12/863 ,  H04L29/08

CPC分类号： H04L43/0894 ,  H04L43/062 ,  H04L43/067 ,  H04L43/0835 ,  H04L47/24 ,  H04L47/2425 ,  H04L47/54 ,  H04L67/10

摘要： A method for scheduling communication traffic in an Advanced Telecom Computing Architecture based equipment and a corresponding apparatus are provided. In the method, communication traffic information of traffic processing means in the equipment is collected; based on the collected communication traffic information, a traffic distribution rule for the traffic processing means in next time period is generated; and based on the generated traffic distribution rule, incoming communication traffic of the equipment is scheduled. The method of the invention considers the quality of service and contracted capacity of the whole equipment and the dynamic processing capability and running environment of the traffic processing means, and can balance the loads of the respective traffic processing means in the equipment with asymmetric multi-application configuration well by adaptively adjusting the distribution of the communication traffic, to guarantee the quality of service of the whole equipment.

摘要翻译： 提供了一种用于在基于高级电信计算架构的设备和相应设备中调度通信业务的方法。 在该方法中，收集设备中的业务处理装置的通信业务信息; 基于所收集的通信交通信息，生成下一时间段中的交通处理装置的交通分配规则; 并基于生成的流量分配规则，调度设备的进入通信流量。 本发明的方法考虑了整个设备的服务质量和承包能力以及交通处理手段的动态处理能力和运行环境，并且可以平衡不对称多应用的设备中各个业务处理手段的负载 配置好，通过自适应调整通信流量的分布，保证整个设备的服务质量。

公开(公告)号：US09163230B2

公开(公告)日：2015-10-20

申请号：US13918979

申请日：2013-06-16

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10

CPC分类号： C12N15/101 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

摘要： The present invention provides a chromatography column and a method for isolating nucleic acid molecules. In one embodiment, the present invention provides a double-layer column of a first anion exchange membrane and a second serially coupled silica membrane. Upon flowing a nucleic acid-containing solution through the first anion exchange membrane, the nucleic acid binds to and then elutes from the first membrane. The eluted solution then flows serially through the second silica membrane, which the nucleic acid binds to and then elutes from. Due to this novel serial coupled double-layer principle, the present invention is particularly suitable for co-isolating RNA and DNA, for isolating nucleic acid embraced by proteins, e.g., viruses, and for isolating diluted nucleic acid in a large volume, e.g., plasma. In addition, the eluted nucleic acid is ready for downstream applications.

摘要翻译： 本发明提供色谱柱和分离核酸分子的方法。 在一个实施方案中，本发明提供了第一阴离子交换膜和第二串联偶联二氧化硅膜的双层柱。 当使含核酸的溶液流过第一阴离子交换膜时，核酸与第一膜结合并然后从第一膜洗脱。 然后洗脱的溶液顺序流过第二个二氧化硅膜，核酸结合然后从其中洗脱出来。 由于这种新颖的串联耦合双层原理，本发明特别适用于共分离RNA和DNA，用于分离蛋白质（例如病毒）所包围的核酸，并用于大体积分离稀释的核酸，例如， 等离子体。 此外，洗脱的核酸准备用于下游应用。

公开(公告)号：US09133491B2

公开(公告)日：2015-09-15

申请号：US13918980

申请日：2013-06-16

申请人： Shaofeng Ding ,  Qiang Liu

发明人： Shaofeng Ding ,  Qiang Liu

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2525/121 ,  C12Q2525/186 ,  C12Q2565/301

摘要： A new method of RNA-PAP was developed that can directly amplify RNA template without additional treatment. RNA-PAP brings in a new mechanism for amplification of RNA template in which RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization are serially coupled using 3′ blocked primers. Due to this serial coupling, RNA-PAP has high selectivity against mismatches on the RNA template, providing highly specific amplification of RNA template. In addition, mutant polymerases were genetically engineered for higher efficiency of RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization.

公开(公告)号：US09021238B2

公开(公告)日：2015-04-28

申请号：US13372103

申请日：2012-02-13

申请人： Xiao Tao Chang ,  Qiang Liu

发明人： Xiao Tao Chang ,  Qiang Liu

IPC分类号： G06F7/38 ,  G06F9/00 ,  G06F9/44 ,  G06F15/00 ,  G06F9/35 ,  G06F9/30 ,  G06F9/38

CPC分类号： G06F9/35 ,  G06F9/30098 ,  G06F9/382

摘要： A data processing system and method are disclosed. The system comprises an instruction-fetch stage where an instruction is fetched and a specific instruction is input into decode stage; a decode stage where said specific instruction indicates that contents of a register in a register file are used as an index, and then, the register file pointed to by said index is accessed based on said index; an execution stage where an access result of said decode stage is received, and computations are implemented according to the access result of the decode stage.

摘要翻译： 公开了一种数据处理系统和方法。 该系统包括指令获取阶段，其中获取指令并将特定指令输入到解码级; 解码阶段，其中所述特定指令指示寄存器文件中的寄存器的内容被用作索引，然后基于所述索引访问由所述索引指向的寄存器文件; 其中接收到所述解码级的访问结果的执行阶段，并且根据解码级的访问结果实现计算。

公开(公告)号：US20140271198A1

公开(公告)日：2014-09-18

申请号：US13802590

申请日：2013-03-13

申请人： Qiang Liu ,  Myles L. Baker

发明人： Qiang Liu ,  Myles L. Baker

IPC分类号： F03D1/06 ,  F03D1/00

CPC分类号： F03D1/0675 ,  B29C65/48 ,  B29C66/1248 ,  B29C66/43 ,  B29C66/721 ,  B29C70/52 ,  B29L2031/085 ,  F03D13/10 ,  F05B2240/302 ,  Y02E10/721 ,  Y02E10/728 ,  Y02P70/523 ,  Y10T29/49337

摘要： Wind turbine blades with layered, multi-component spars, and associated systems and methods are disclosed. A wind turbine blade system in accordance with a particular embodiment includes a first blade segment having a first spar element that includes first planks having a first thickness and a first plank composition, and a second blade segment having a second spar element that includes second planks having a second thickness and a second plank composition different than the first plank composition. The second blade segment is joined to the first blade segment at a joint, and, in particular embodiments, an overall product of thickness and elastic modulus of the first planks is approximately equal to an overall product of thickness and elastic modulus for the second planks.

摘要翻译： 公开了具有分层，多组分翼梁以及相关系统和方法的风力涡轮机叶片。 根据特定实施例的风力涡轮机叶片系统包括具有第一翼梁元件的第一叶片段，所述第一翼梁元件包括具有第一厚度的第一木板和第一木板组合物，以及具有第二翼梁元件的第二叶片段，所述第二翼梁元件包括第二木板， 第二厚度和与第一板组合物不同的第二板组合物。 第二刀片段在接头处连接到第一刀片段，并且在具体实施例中，第一板的厚度和弹性模量的总体乘积近似等于第二板的厚度和弹性模量的总积。