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    OLIGORIBONUCLEOTIDES AND RIBONUCLEASES FOR CLEAVING RNA
    52.
    发明申请
    OLIGORIBONUCLEOTIDES AND RIBONUCLEASES FOR CLEAVING RNA 审中-公开
    用于清除RNA的寡核苷酸和核糖核酸

    公开(公告)号：US20100151458A1

    公开(公告)日：2010-06-17

    申请号：US12502050

    申请日：2009-07-13

    申请人： Stanley T. Crooke

    发明人： Stanley T. Crooke

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C12N5/02

    CPC分类号： C12N15/113 ,  A61K38/00 ,  C07H21/00 ,  C12N9/22 ,  C12N15/1135 ,  C12N2310/311 ,  C12N2310/312 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/316 ,  C12N2310/318 ,  C12N2310/3181 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3341 ,  C12N2310/335 ,  C12N2310/341 ,  C12N2310/346 ,  Y02A50/395 ,  Y02P20/582 ,  C12N2310/3527 ,  C12N2310/3525 ,  C12N2310/3521

    摘要： Oligomeric compounds including oligoribonucleotides and oligoribonucleosides are provided that have subsequences of 2′-pentoribofuranosyl nucleosides that activate dsRNase. The oligoribonucleotides and oligoribonucleosides can include substituent groups for increasing binding affinity to complementary nucleic acid strand as well as substituent groups for increasing nuclease resistance. The oligomeric compounds are useful for diagnostics and other research purposes, for modulating the expression of a protein in organisms, and for the diagnosis, detection and treatment of other conditions susceptible to oligonucleotide therapeutics. Also included in the invention are mammalian ribonucleases, i.e., enzymes that degrade RNA, and substrates for such ribonucleases. Such a ribonuclease is referred to herein as a dsRNase, wherein “ds” indicates the RNase's specificity for certain double-stranded RNA substrates. The artificial substrates for the dsRNases described herein are useful in preparing affinity matrices for purifying mammalian ribonuclease as well as non-degradative RNA-binding proteins.

    摘要翻译： 提供了包含寡核糖核苷酸和寡核糖核苷的寡聚化合物，其具有激活dsRNase的2'-戊二呋呋糖基核苷的亚序列。 寡核糖核苷酸和寡核糖核苷可以包括用于增加对互补核酸链的结合亲和力的取代基以及用于增加核酸酶抗性的取代基。 寡聚化合物可用于诊断和其他研究目的，用于调节生物体中的蛋白质的表达，以及用于诊断，检测和治疗对寡核苷酸治疗剂敏感的其它病症。 本发明还包括哺乳动物核糖核酸酶，即降解RNA的酶和这种核糖核酸酶的底物。 这样的核糖核酸酶在本文中称为dsRNase，其中“ds”表示RNase对某些双链RNA底物的特异性。 本文所述的dsR酶的人造底物可用于制备用于纯化哺乳动物核糖核酸酶以及非降解性RNA结合蛋白的亲和基质。

    Mass spectrometric methods for biomolecular screening
    56.
    发明授权
    Mass spectrometric methods for biomolecular screening 失效
    用于生物分子筛选的质谱法

    公开(公告)号：US06428956B1

    公开(公告)日：2002-08-06

    申请号：US09076206

    申请日：1998-05-12

    申请人： Stanley T. Crooke ,  Richard Griffey ,  Steve Hofstadler

    发明人： Stanley T. Crooke ,  Richard Griffey ,  Steve Hofstadler

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6811 ,  C40B40/06 ,  H01J49/004 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2525/101 ,  C12Q2565/133 ,  C12Q2525/121 ,  C12Q2565/607

    摘要： The present invention provides methods for the determination of the structure of biomolecular targets, as well as the site and nature of the interaction between ligands and biomolecular targets. The present invention also provides methods for the determination of the relative affinity of a ligand for the biomolecular target it interacts with. Also provided are methods for screening ligand or combinatorial libraries of compounds against one or more than one biological target molecules. The methods of the invention also allow determination of the relative binding affinity of combinatorial and other compounds for a biomolecular target. The present invention further provides methods for the use of mass modifying tags for screening multiple biomolecular targets. In a preferred embodiment, ligands which have great specificity and affinity for molecular interaction sites on biomolecules, especially RNA can be identified. In preferred embodiments, such identification can be made simultaneously with libraries of ligands.

    摘要翻译： 本发明提供了用于确定生物分子靶标的结构的方法，以及配体和生物分子靶标之间的相互作用的位点和性质。 本发明还提供了确定配体与其相互作用的生物分子靶的相对亲和力的方法。 还提供了用于筛选针对一种或多于一种生物靶分子的化合物的配体或组合文库的方法。 本发明的方法还允许确定组合和其它化合物对于生物分子靶标的相对结合亲和力。 本发明还提供使用质量修饰标签筛选多个生物分子靶标的方法。 在优选的实施方案中，可以鉴定对生物分子，特别是RNA上的分子相互作用位点具有极大特异性和亲和力的配体。 在优选的实施方案中，这种鉴定可以与配体文库同时进行。