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    Method of decreasing liquiritigenin-derived isoflavones relative to total isoflavones in plants and plants producing reduced ratio of liquiritigenin-derived isoflavones relative to total isoflavones
    52.
    发明授权
    Method of decreasing liquiritigenin-derived isoflavones relative to total isoflavones in plants and plants producing reduced ratio of liquiritigenin-derived isoflavones relative to total isoflavones 有权
    降低甘草素衍生异黄酮相对于植物和植物中总异黄酮的方法，产生相对于总异黄酮的甘草素衍生异黄酮的比例降低

    公开(公告)号：US07323621B2

    公开(公告)日：2008-01-29

    申请号：US10734947

    申请日：2003-12-11

    申请人： Brian McGonigle

    发明人： Brian McGonigle

    IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/63 ,  C12N15/82

    CPC分类号： C12N9/0004 ,  C12N15/8243 ,  C12N15/825

    摘要： This invention pertains to methods for decreasing the ratio of liquiritigenin-derived isoflavones relative to total isoflavone levels in isoflavonoid-producing plants and plant parts by transforming plants with a recombinant DNA construct comprising a nucleic acid sequence of at least 200 nucleotides and having at least 75% sequence identity to a polynucleotide encoding a chalcone reductase. More preferably, this invention pertains to methods for decreasing the ratios of daidzein and glycitein and their conjugates relative to total isoflavone levels in soybean plants and soybean plant parts by transforming plants with a recombinant DNA construct comprising a nucleic acid sequence of at least 200 nucleotides and having at least 75% sequence identity to a polynucleotide encoding a chalcone reductase.

    摘要翻译： 本发明涉及通过用包含至少200个核苷酸的核酸序列并且具有至少75个核苷酸的重组DNA构建体转化植物来降低产甘草素衍生物的异黄酮相对于异黄酮生成植物和植物部分中总异黄酮水平的比例的方法 ％编码查耳酮还原酶的多核苷酸序列同一性。 更优选地，本发明涉及通过用包含至少200个核苷酸的核酸序列的重组DNA构建体转化植物来降低大豆植物和大豆植物部分中黄豆苷原和黄豆黄素及其缀合物相对于总异黄酮水平的比例的方法，以及 与编码查耳酮还原酶的多核苷酸具有至少75％的序列同一性。

    Transgenic soybean seeds having reduced activity of lipoxygenases
    57.
    发明申请
    Transgenic soybean seeds having reduced activity of lipoxygenases 审中-公开
    具有降低脂氧合酶活性的转基因大豆种子

    公开(公告)号：US20060005276A1

    公开(公告)日：2006-01-05

    申请号：US11076733

    申请日：2005-03-09

    申请人： Saverio Falco ,  Brian McGonigle ,  Carl Maxwell

    发明人： Saverio Falco ,  Brian McGonigle ,  Carl Maxwell

    IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8243 ,  C12N15/8247

    摘要： The present invention concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases, the transgenic soybean plant having a nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the nucleic acid fragment is capable of suppressing expression of native seed lipoxygenases and has been introduced into the soybean plant by transformation. The present invention also concerns a transgenic soybean plant producing seed having reduced activity of seed lipoxygenases and a second native enzyme, when compared to a soybean plant expressing wild type activity of native seed lipoxygenases and the second native enzyme, the transgenic soybean plant having a first nucleic acid fragment from at least a portion of at least one soybean seed lipoxygenase gene, wherein the first nucleic acid fragment is capable of suppressing expression of said native seed lipoxygenases, and a second nucleic acid fragment from at least a portion of at least one second native enzyme gene, wherein the second nucleic acid fragment is capable of suppressing expression of the native second enzyme, wherein the first nucleic acid fragment and the second nucleic acid fragment have been introduced into the soybean plant by transformation, and wherein the second enzyme is selected from the group consisting of an enzyme of the lipid oxidation pathway, fatty acid desaturation pathway, phenylpropanoid pathway, triterpenoid pathway, and combinations thereof. Methods of suppressing wild type activity of native soybean seed lipoxygenases, alone or in combination with suppression of a second native enzyme are also embodied by the present invention.

    摘要翻译： 本发明涉及与表达天然种子脂氧合酶的野生型活性的大豆植物相比，产生种子脂氧合酶活性降低的转基因大豆植物，转基因大豆植物具有至少一部分至少一种的核酸片段 大豆种子脂氧合酶基因，其中所述核酸片段能够抑制天然种子脂氧合酶的表达，并通过转化被引入大豆植物。 本发明还涉及当与表达天然种子脂氧合酶和第二天然酶的野生型活性的大豆植物相比时，产生种子脂氧合酶和第二天然酶活性降低的转基因大豆植物，转基因大豆植物具有第一 至少一部分大豆种子脂氧合酶基因的核酸片段，其中所述第一核酸片段能够抑制所述天然种子脂氧合酶的表达，以及第二核酸片段至少部分至少一个第二 天然酶基因，其中第二核酸片段能够抑制天然的第二酶的表达，其中通过转化将第一核酸片段和第二核酸片段引入大豆植物中，并且其中选择第二种酶 来自由脂质氧化途径的酶组成的组，脂肪酸des 致敏途径，苯丙素途径，三萜途径及其组合。 本发明也体现了抑制天然大豆种子脂氧合酶单独或与抑制第二天然酶结合的方法。

    Method of improving soybean protein color by reducing levels of flavonol
    58.
    发明申请
    Method of improving soybean protein color by reducing levels of flavonol 审中-公开
    通过降低黄酮醇水平来改善大豆蛋白色的方法

    公开(公告)号：US20050198710A1

    公开(公告)日：2005-09-08

    申请号：US10819773

    申请日：2004-04-07

    申请人： Brian McGonigle ,  Carl Maxwell ,  Aideen Hession

    发明人： Brian McGonigle ,  Carl Maxwell ,  Aideen Hession

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/82

    CPC分类号： C12N9/0071 ,  C12N15/8218 ,  C12N15/8243

    摘要： Recombinant constructs for reducing flavonol levels in a flavonol-producing plants are described. Such constructs comprise a promoter operably linked to a stem-loop structure wherein a nucleic acid sequence of at least 200 nucleotides and having at least 75% sequence identity to SEQ ID NO:4 forms part of loop part of the structure. Methods for reducing flavonol levels are also described.

    摘要翻译： 描述了用于在黄酮醇生产植物中降低黄酮醇水平的重组构建体。 这样的构建体包括可操作地连接到茎 - 环结构的启动子，其中至少200个核苷酸并且与SEQ ID NO：4具有至少75％的序列同一性的核酸序列形成结构的环部分的一部分。 还描述了降低黄酮醇水平的方法。