摘要:
A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a fluid containing cells, a lysis reagent reservoir containing a lysis reagent and having a valve, and, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, the lysis section having a lysis heater for heating the fluid in the lysis section, wherein, the valve is upstream of the lysis section and configured to open as the fluid flows into the lysis section thereby adding the lysis reagent to the fluid.
摘要:
A test module for lysing cells in a fluid, the test module having an outer casing with receptacle for receiving a sample that is a fluid containing cells, a lysis section in fluid communication with the receptacle for holding the fluid during lysis of the cells, and, a lysis heater for heating the fluid in the lysis section to lyse cells, wherein, the test module is configured for drawing the fluid from the receptacle to the lysis section by capillary action.
摘要:
A lab-on-a-chip (LOC) device for amplifying nucleic acid sequences, the LOC device having an inlet for receiving a sample containing genetic material, a supporting substrate, a plurality of reagent reservoirs, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the nucleic acid amplification section is supported on the supporting substrate.
摘要:
A lab-on-a-chip (LOC) device for genetic analysis of a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating smaller constituents from larger constituents, the smaller constituents being smaller than a predetermined size threshold, and the larger constituents being larger than the predetermined size threshold, and, a nucleic acid amplification section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section and the nucleic acid amplification section are both supported on the supporting substrate.
摘要:
A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing dNTPs, primers, recombinase, DNA polymerase and buffer solution for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for maintaining the amplification mixture at a predetermined temperature during recombinase polymerase amplification (RPA) of the target nucleic acid sequences.
摘要:
A lab-on-a-chip (LOC) device for genetic analysis of a sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase derived from a thermophilic bacterium for addition to the sample to form an amplification mixture, and, a nucleic acid amplification section for controlling the temperature of the amplification mixture during amplification of the target nucleic acid sequences.
摘要:
A lab-on-a-chip (LOC) device for genetic analysis of a whole blood sample containing target nucleic acid sequences, the LOC device having a sample inlet for receiving the whole blood sample, a plurality of reagent reservoirs containing buffer, dNTPs, primers, and polymerase for addition to the whole blood sample, and, a polymerase chain reaction (PCR) section for thermal cycling the whole blood sample, buffer, dNTPs, primers, and polymerase to amplify the target nucleic acid sequences.
摘要:
A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section with a PCR microchannel for thermally cycling a PCR mixture of the sample, polymerase, dNTP's and buffer solution to amplify the nucleic acid sequences, the PCR microchannel defining a flow-path for the sample such that flow of the sample along the PCR microchannel is driven by capillary action, wherein, the PCR mixture has a volume less than 400 nanoliters.
摘要:
A lab-on-a-chip (LOC) device for detecting pathogens in a biological sample, the LOC device having an inlet for receiving the sample, a supporting substrate, a dialysis section for separating pathogens from larger constituents in the sample, a plurality of reagent reservoirs, a lysis section downstream of the dialysis section for lysing the pathogens to release genetic material therein, the lysis section being in fluid communication with one of the reagent reservoirs containing a lysis reagent for lysing the cells in the lysis section, and, a nucleic acid amplification section downstream of the lysis section for amplifying nucleic acid sequences in the genetic material, wherein, the dialysis section, the lysis section and the nucleic acid amplification section are all supported on the supporting substrate.
摘要:
A microfluidic device having a sample inlet for receiving a sample of biological material having nucleic acid sequences, a polymerase chain reaction (PCR) section for amplifying the nucleic acid sequences, the PCR section having at least one elongate PCR chamber having a longitudinal extent much greater than its lateral dimensions, and, at least one elongate heater element for heating the nucleic acid sequences within the elongate PCR chamber, wherein, the elongate heater element extends parallel with the longitudinal extent of the PCR chamber.