公开(公告)号：US08143369B2

公开(公告)日：2012-03-27

申请号：US12770857

申请日：2010-04-30

申请人： Masaki Fujiwara ,  James Lupton Hedrick ,  Daniel Paul Sanders ,  Yoshiharu Terui ,  Manabu Yasumoto

发明人： Masaki Fujiwara ,  James Lupton Hedrick ,  Daniel Paul Sanders ,  Yoshiharu Terui ,  Manabu Yasumoto

IPC分类号： C08G64/00 ,  C08G63/00

CPC分类号： C08G64/1633 ,  C07D319/06 ,  C08G63/08 ,  C08G63/64 ,  C08G64/0233 ,  C08G64/0241 ,  C08G64/025 ,  C08G64/38 ,  C08G64/42 ,  C08G2261/126 ,  Y02P20/582

摘要： A one pot method of preparing cyclic carbonyl compounds comprising an active pendant pentafluorophenyl ester group is disclosed. The cyclic carbonyl compounds can be polymerized by ring opening methods to form ROP polymers comprising repeat units comprising a side chain pentafluorophenyl ester group. Using a suitable nucleophile, the pendant pentafluorophenyl ester group can be selectively transformed into a variety of other functional groups before or after the ring opening polymerization.

摘要翻译： 公开了一种制备包含活性五氟苯基酯基团的环状羰基化合物的一锅法。 环状羰基化合物可以通过开环方法聚合以形成包含包含侧链五氟苯酯基的重复单元的ROP聚合物。 使用合适的亲核试剂，可以在开环聚合前后选择性地将五氟苯基酯基团转变成多种其它官能团。

公开(公告)号：US08088271B2

公开(公告)日：2012-01-03

申请号：US10578988

申请日：2004-12-03

申请人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

发明人： Masaki Fujiwara ,  Teppei Shinno ,  Shin Ikeda

IPC分类号： G01N27/26 ,  G01N33/487

CPC分类号： G01N27/3274

摘要： The present invention provides a method of electrochemically measuring a hematocrit (Hct) value using a sensor, capable of achieving excellent measurement accuracy and reliability and also provides a sensor used in the method. The method of electrochemically measuring a hematocrit (Hct) value of blood include: providing an electrode system having a working electrode (11) and a counter electrode (12), in which a redox substance is provided on the counter electrode (12) but not on the working electrode (11); supplying blood to the electrode system; applying a voltage to the electrode system in this state to cause an oxidation current or a reduction current to flow between the working electrode (11) and the counter electrode (12); detecting the oxidation current or the reduction current; and determining a Hct value based on a value of the detected current.

摘要翻译： 本发明提供一种使用传感器电化学测量血细胞比容（Hct）值的方法，其能够实现优异的测量精度和可靠性，并且还提供了该方法中使用的传感器。 电化学测量血液血细胞比容（Hct）值的方法包括：提供具有工作电极（11）和对电极（12）的电极系统，其中在对电极（12）上设置氧化还原物质 在工作电极（11）上; 向电极系统供应血液; 在该状态下向电极系统施加电压，使得氧化电流或还原电流在工作电极（11）和对电极（12）之间流动。 检测氧化电流或还原电流; 以及基于检测到的电流的值确定Hct值。

公开(公告)号：US20110278167A1

公开(公告)日：2011-11-17

申请号：US13135848

申请日：2011-07-15

申请人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

发明人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

IPC分类号： G01N27/327

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  G01N33/48771 ,  Y10T29/49002

摘要： A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1. The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.

摘要翻译： 根据本发明的生物传感器包括支撑体1，由诸如金，钯等的诸如贵金属的导电材料构成的导电层2和碳，平行于载体侧的狭缝3a和3b 如图1所示，与支撑体1侧垂直的狭缝4a，4b，工作电极5，对电极6，检测电极7，覆盖工作电极5的间隔件8，对置电极6和检测电极7 支撑体1，形成试样供给路径的矩形切口部9，试料供给路径的入口9a，通过将包含酶等的试剂涂布在工作电极5上而形成的试剂层12，对电极6和 从间隔件8的切口部9暴露的检测电极7和覆盖间隔件8的盖13，如图1所示。 如此构造的生物传感器可以通过简单的方法形成，并且测量精度优异的生物传感器以及试剂层均匀地放置在电极上而不考虑试剂液体组成的生物传感器，并且其中 具有统一的性能可以实现。

公开(公告)号：US20110272294A1

公开(公告)日：2011-11-10

申请号：US13144790

申请日：2010-01-28

申请人： Masaki Fujiwara

发明人： Masaki Fujiwara

IPC分类号： G01N27/416

CPC分类号： G01N27/3274 ,  B01L3/502 ,  B01L2200/147 ,  B01L2300/0645 ,  B01L2400/0406 ,  C12Q1/004 ,  C12Q1/006 ,  C12Q1/54 ,  G01K7/00 ,  G01N27/26 ,  G01N27/28 ,  G01N27/327 ,  G01N27/3272 ,  G01N27/3273 ,  G01N27/416 ,  Y10T436/144444

摘要： A sensor chip (200) is configured to measure the temperature of a blood sample and includes a capillary section (40) and an electrode unit (11, 12). The capillary section (40) allows the blood sample to be introduced therein. The electrode unit (11, 12) is configured to measure the temperature of the blood sample and includes a working electrode and a counter electrode. The working electrode and the counter electrode respectively include a reaction reagent layer (20) containing an electrolyte. Further, the electrode unit (11, 12) is configured to receive a predetermined voltage to be applied in measuring the temperature of the blood sample for allowing a result of the measurement to be less affected by increase and reduction in a glucose concentration and the like.

摘要翻译： 传感器芯片（200）被配置为测量血液样本的温度，并且包括毛细管部分（40）和电极单元（11,12）。 毛细管部分（40）允许将血液样品引入其中。 电极单元（11,12）被配置为测量血液样本的温度，并且包括工作电极和对电极。 工作电极和对电极分别包括含有电解质的反应试剂层（20）。 此外，电极单元（11,12）被配置为在测量血液样本的温度时接受预定的电压以允许测量结果受到葡萄糖浓度等的增加和降低的影响较小 。

公开(公告)号：US20110269917A1

公开(公告)日：2011-11-03

申请号：US12770877

申请日：2010-04-30

申请人： Masaki Fujiwara ,  James L. Hedrick ,  Daniel P. Sanders ,  Manabu Yasumoto

发明人： Masaki Fujiwara ,  James L. Hedrick ,  Daniel P. Sanders ,  Manabu Yasumoto

IPC分类号： C08G64/00 ,  C07D317/24 ,  C08L69/00 ,  C07D319/06

CPC分类号： C07D319/04 ,  C07D317/36 ,  C08G64/0233 ,  C08G64/30 ,  Y02P20/582

摘要： A one pot method of preparing cyclic carbonyl compounds comprising an active pendant pentafluorophenyl carbonate group is disclosed. The cyclic carbonyl compounds can be polymerized by ring opening methods to form ROP polymers comprising repeat units comprising a side chain pentafluorophenyl carbonate group. Using a suitable nucleophile, the pendant pentafluorophenyl carbonate group can be selectively transformed into a variety of other functional groups before or after the ring opening polymerization.

摘要翻译： 公开了一种制备包含活性五氟苯基碳酸酯基团的环状羰基化合物的一锅法。 环状羰基化合物可以通过开环方法聚合以形成包含包含侧链五氟苯基碳酸酯基团的重复单元的ROP聚合物。 使用合适的亲核试剂，可以在开环聚合之前或之后将五氟苯基五氟苯基碳酸酯基团选择性地转化成多种其它官能团。

公开(公告)号：US07998325B2

公开(公告)日：2011-08-16

申请号：US10809217

申请日：2004-03-25

申请人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

发明人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

IPC分类号： G01N27/327

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  G01N33/48771 ,  Y10T29/49002

摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

摘要翻译： 公开了一种生物传感器，其包括支撑件; 由诸如金或钯等贵金属的导电材料和碳组成的导电层; 平行于和垂直于支撑体侧面的狭缝; 工作，计数器和检测电极; 覆盖支架上的工作电极，反电极和检测电极的间隔件; 在间隔件中形成矩形切口，形成试样供应路径; 样品供应路径的入口; 通过将含有酶的试剂施加到通过所述间隔件中的切口暴露的所述工作，反向和检测电极而形成的试剂层; 和间隔物上的盖子。 可以通过简单的方法形成生物传感器，并且不管试剂组成如何，在电极上提供均匀的试剂层。

公开(公告)号：US20110174613A1

公开(公告)日：2011-07-21

申请号：US12930627

申请日：2011-01-11

申请人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

发明人： Shoji Miyazaki ,  Hiroyuki Tokunaga ,  Masaki Fujiwara ,  Eriko Yamanishi ,  Yoshinobu Tokuno

IPC分类号： G01N27/403

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  G01N33/48771 ,  Y10T29/49002

摘要： A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

公开(公告)号：US20110034721A1

公开(公告)日：2011-02-10

申请号：US12678250

申请日：2008-09-05

申请人： Yuji Hagiwara ,  Masashi Nagamori ,  Masaki Fujiwara ,  Jonathan Joachim Jodry ,  Satoru Narizuka

发明人： Yuji Hagiwara ,  Masashi Nagamori ,  Masaki Fujiwara ,  Jonathan Joachim Jodry ,  Satoru Narizuka

IPC分类号： C07C69/74

CPC分类号： C07C309/17 ,  C07C29/147 ,  C07C67/08 ,  C07C67/14 ,  C07C303/32 ,  C07C309/08 ,  C07C309/12 ,  C07C313/04 ,  C07C381/12 ,  C07C2602/42 ,  C07C2603/74 ,  G03F7/0045 ,  G03F7/0046 ,  C07C31/34 ,  C07C69/653 ,  C07C69/63 ,  C07C69/753

摘要： By using an organic base when a carboxylic acid bromodifluoroethyl ester is sulfinated by using a sulfinating agent, there is obtained 2-(alkylcarbonyloxy)-1,1-difluoroethanesulfinic acid ammonium salt. By oxidizing the 2-(alkylcarbonyloxy)-1,1-difluoroethanesulfinic acid ammonium salt, there is obtained 2-(alkylcarbonyloxy)-1,1-difluoroethanesulfonic acid ammonium salt. By using the 2-(alkylcarbonyloxy)-1,1-difluoroethanesulfonic acid ammonium salt as a raw material and exchanging it into an onium salt directly or through saponification/esterification, there can be obtained a 2-alkylcarbonyloxy-1,1-difluoroethanesulfonic acid onium salt.

摘要翻译： 通过使用硫酸化剂将羧酸溴二氟乙酯磺化时，通过使用有机碱，得到2-（烷基羰氧基）-1,1-二氟乙烷亚磺酸铵盐。 通过氧化2-（烷基羰基氧基）-1,1-二氟乙烷亚磺酸铵盐，得到2-（烷基羰氧基）-1,1-二氟乙磺酸铵盐。 通过使用2-（烷基羰氧基）-1,1-二氟乙磺酸铵盐作为原料，直接或通过皂化/酯化将其交换为鎓盐，可以得到2-烷基羰基氧基-1,1-二氟乙磺酸 盐盐

公开(公告)号：US20110015431A1

公开(公告)日：2011-01-20

申请号：US12678223

申请日：2008-09-05

申请人： Jonathan Joachim Jodry ,  Masashi Nagamori ,  Yuji Hagiwara ,  Masaki Fujiwara ,  Satoru Narizuka

发明人： Jonathan Joachim Jodry ,  Masashi Nagamori ,  Yuji Hagiwara ,  Masaki Fujiwara ,  Satoru Narizuka

IPC分类号： C07C67/16 ,  C07C309/08 ,  C07C309/10 ,  C07C29/143

CPC分类号： C07C309/17 ,  C07C29/147 ,  C07C67/08 ,  C07C67/14 ,  C07C303/32 ,  C07C309/08 ,  C07C309/12 ,  C07C313/04 ,  C07C381/12 ,  C07C2602/42 ,  C07C2603/74 ,  G03F7/0045 ,  G03F7/0046 ,  C07C31/34 ,  C07C69/653 ,  C07C69/63 ,  C07C69/753

摘要： Disclosed is a process for producing 2-bromo-2,2-difluoroethanol, which comprises reducing a bromodifluoroacetic acid derivative represented by the formula [1] by using an ate hydride complex as a reducing agent. 2-Bromo-2,2-difluoroethanol thus produced can be used as the starting material to carry out the esterification step, the sulfination step and the oxidation step in this order, thereby producing a 2-alkylcarbonyloxy-1,1-difluoroethanesulfonic acid salt, wherein A represents a substituted or unsubstituted linear, branched or cyclic alkoxy group having 1 to 20 carbon atoms, a substituted or unsubstituted aryloxy group having 6 to 15 carbon atoms, a heteroaryloxy group having 4 to 15 carbon atoms, or a halogen atom.

摘要翻译： 公开了一种2-溴-2,2-二氟乙醇的制备方法，其包括使用氢化物​​络合物作为还原剂还原式[1]表示的溴二氟乙酸衍生物。 可以使用如此制备的2-溴-2,2-二氟乙醇作为起始原料，依次进行酯化步骤，硫化步骤和氧化步骤，由此制备2-烷基羰基氧基-1,1-二氟乙磺酸盐 其中A表示取代或未取代的具有1至20个碳原子的直链，支链或环状烷氧基，取代或未取代的碳原子数6〜15的芳氧基，碳原子数4〜15的杂芳氧基或卤素原子。

公开(公告)号：US20100243476A1

公开(公告)日：2010-09-30

申请号：US12377514

申请日：2007-10-17

申请人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

发明人： Masaki Fujiwara ,  Shin Ikeda ,  Takahiro Nakaminami

IPC分类号： G01N27/26 ,  G01N33/50

CPC分类号： G01N27/3274 ,  G01N27/3272 ,  G01N27/413

摘要： Voltage is applied across a counter electrode and a working electrode, with which a blood sample is in contact, in such a state that an oxidant in a redox substance is not substantially in contact with a working electrode but is in contact with a counter electrode and a reductant is not substantially in contact with the counter electrode but is in contact with the working electrode, whereby the reductant and the oxidant are respectively oxidized and reduced to measure current produced upon the oxidation and reduction. According to the above constitution, while lowering the voltage applied across the working electrode and the counter electrode, the Hct value of the blood sample can be measured stably with a satisfactory detection sensitivity. This measurement can be carried out with a sensor chip comprising a working electrode (11), a counter electrode (12), and a blood sample holding part (14) having branch parts (18a, 18b). A first reagent (13) consisting essentially of an oxidant in a redox substance is disposed in contact with the surface of a counter electrode which faces the branch part (18b), and a second reagent (19) consisting essentially of a reductant is disposed in contact with the surface of a working electrode that faces the branch part (18a).

摘要翻译： 在氧化还原物质中的氧化剂基本上不与工作电极接触但与对电极接触的状态下，跨越对置电极和与血液样品接触的工作电极施加电压， 还原剂基本上不与对电极接触，而是与工作电极接触，由此还原剂和氧化剂分别被氧化和还原，以测量在氧化和还原时产生的电流。 根据上述结构，在降低施加在工作电极和对电极两端的电压的同时，能够以良好的检测灵敏度稳定地测定血液样品的Hct值。 该测量可以用包括工作电极（11），对电极（12）和具有分支部分（18a，18b）的血液样本保持部分（14）的传感器芯片进行。 基本上由氧化还原物质中的氧化剂组成的第一试剂（13）被设置成与面对分支部分（18b）的对电极的表面接触，并且基本上由还原剂组成的第二试剂（19）设置在 与面向分支部（18a）的工作电极的表面接触。