公开(公告)号：US10233217B2

公开(公告)日：2019-03-19

申请号：US14884469

申请日：2015-10-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Sara A. Salvador ,  Jinling Wang ,  Stanislaw Flasinski ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts

IPC: C07K14/325 ,  A01N47/08 ,  A01N63/02 ,  C12N15/82 ,  C12N15/62

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US20180100000A1

公开(公告)日：2018-04-12

申请号：US15727883

申请日：2017-10-09

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Uma Kesanapalli

IPC: C07K14/325 ,  C12Q1/6895 ,  G01N33/68 ,  C12N15/82 ,  A01N37/18

CPC classification number: C07K14/325 ,  A01N37/18 ,  A01N37/46 ,  C12N15/8286 ,  C12Q1/6895 ,  G01N33/5308 ,  G01N33/68 ,  G01N2333/325 ,  G01N2333/43552 ,  Y02A40/162

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.

公开(公告)号：US20180087068A1

公开(公告)日：2018-03-29

申请号：US15835346

申请日：2017-12-07

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  A01N63/02 ,  C07K14/325 ,  A01N47/08

CPC classification number: C12N15/8286 ,  A01N47/08 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20160108428A1

公开(公告)日：2016-04-21

申请号：US14884469

申请日：2015-10-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C12N15/82 ,  A01N63/02 ,  A01N47/08 ,  C07K14/325

CPC classification number: C07K14/325 ,  A01N47/08 ,  A01N63/02 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/8286 ,  Y02A40/162

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Abstract translation: 公开编码显示鳞翅目抑制活性的新型嵌合杀虫蛋白的核苷酸序列。 具体实施方案提供了组合物和转化的植物，植物部分和含有编码一种或多种嵌合杀虫蛋白的重组核酸分子的种子。

公开(公告)号：US20250002932A1

公开(公告)日：2025-01-02

申请号：US18830256

申请日：2024-09-10

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Jason S. Milligan ,  James K. Roberts ,  Yong Yin

IPC: C12N15/82

Abstract: Pesticidal proteins exhibiting toxic activity against Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran pest species are disclosed, and include, but are not limited to, TIC6280, TIC6281, TIC6282, TIC6283, TIC8808, TIC9480, TIC9257, TIC7106, TIC7017, TIC7107, TIC7108, TIC7109, TIC7110, TIC7111, TIC7589, TIC9258, and TIC9259. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the pesticidal proteins provided. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran, Coleopteran, Hemipteran and Thysanopteran infestation are provided which contain recombinant nucleic acid sequences encoding the disclosed pesticidal proteins. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Coleopteran, Lepidopteran, Hemipteran, and Thysanopteran species pests using the disclosed pesticidal proteins are also provided.

公开(公告)号：US20240352480A1

公开(公告)日：2024-10-24

申请号：US18628578

申请日：2024-04-05

Applicant: Monsanto Technology LLC

Inventor： David J. Bowen ,  Catherine A. Chay ,  Todd A. Ciche ,  Stanislaw Flasinski ,  Arlene R. Howe ,  Krishnakumar Sridharan

IPC: C12N15/82 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32

CPC classification number: C12N15/8286 ,  A01N37/46 ,  A01N63/50 ,  C07K14/195 ,  C07K14/32 ,  C07K2319/00

Abstract: A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

公开(公告)号：US20240287539A1

公开(公告)日：2024-08-29

申请号：US18426976

申请日：2024-01-30

Applicant: Monsanto Technology LLC

Inventor： Gregory J. Bean ,  David J. Bowen ,  Catherine A. Chay ,  Arlene R. Howe ,  Jason S. Milligan ,  Yong Yin

IPC: C12N15/82 ,  A01N63/50 ,  C07K14/195

CPC classification number: C12N15/8286 ,  A01N63/50 ,  C07K14/195

Abstract: Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

公开(公告)号：US20240247280A1

公开(公告)日：2024-07-25

申请号：US18404247

申请日：2024-01-04

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  A01N47/08 ,  A01N63/50 ,  C07K14/325

CPC classification number: C12N15/8286 ,  A01N47/08 ,  A01N63/50 ,  C07K14/325 ,  Y02A40/146

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US20240200092A1

公开(公告)日：2024-06-20

申请号：US18529422

申请日：2023-12-05

Applicant: Monsanto Technology LLC

Inventor： Erin L. Hall ,  Arlene R. Howe ,  Brent A. O'Brien ,  James K. Roberts ,  Francesca L. Stubbins ,  Stephanie C. Waldheuser

IPC: C12N15/82 ,  A01N63/20 ,  A01N63/50 ,  A01P7/04

CPC classification number: C12N15/8286 ,  A01N63/20 ,  A01N63/50 ,  A01P7/04 ,  C12N15/8205

Abstract: Pesticidal protein exhibiting toxic activity against Hemipteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC11207 and TIC11304. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the disclosed pesticidal protein. Transgenic plants, plant cells, seed, and plant parts resistant to Hemipteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the protein of the present invention in a biological sample, and methods of controlling Hemipteran and Lepidopteran species pests using TIC11207 and TIC11304 pesticidal proteins are also provided.

公开(公告)号：US11987603B2

公开(公告)日：2024-05-21

申请号：US17698936

申请日：2022-03-18

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C07K14/325 ,  A01N37/46 ,  C12N15/82 ,  C12N15/62

CPC classification number: C07K14/325 ,  A01N37/46 ,  C12N15/8286 ,  C07K2319/00 ,  C12N15/62 ,  Y02A40/146

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.