公开(公告)号：US10174340B2

公开(公告)日：2019-01-08

申请号：US15194355

申请日：2016-06-27

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/6888

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US10167484B2

公开(公告)日：2019-01-01

申请号：US15130684

申请日：2016-04-15

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Larry A. Gilbertson ,  David K. Kovalic ,  Thomas J. LaRosa ,  Maolong Lu ,  Tichafa R. I. Munyikwa ,  James K. Roberts ,  Wei Wu ,  Bei Zhang

IPC: C12N15/82 ,  A01N63/02

Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US09988642B2

公开(公告)日：2018-06-05

申请号：US14714068

申请日：2015-05-15

Applicant: Monsanto Technology LLC

Inventor： Andrey A. Boukharov ,  Zijin Du ,  Liang Guo ,  Michelle C. Hresko ,  David K. Kovalic ,  Zhaolong Li ,  Maolong Lu ,  James P. McCarter ,  Nancy M. Miller ,  Mark Vaudin ,  Deryck J. Williams ,  Wei Wu

IPC: C12N15/82 ,  C07K14/435 ,  A61K31/713 ,  C12N15/113 ,  A23L33/105 ,  A01N57/16 ,  C12Q1/68

CPC classification number: C12N15/8285 ,  A01N57/16 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/713 ,  C07K14/435 ,  C07K14/4354 ,  C12N15/113 ,  C12N15/1137 ,  C12N15/8261 ,  C12N15/8273 ,  C12N2310/11 ,  C12Q1/6888 ,  Y02A40/164

Abstract: The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

公开(公告)号：US09663793B2

公开(公告)日：2017-05-30

申请号：US13830403

申请日：2013-03-14

Applicant: Monsanto Technology LLC

Inventor： Jeffrey E. Ahrens ,  Shoba Cherian ,  Paul J. Loida ,  Linda L. Lutfiyya ,  Wei Wu ,  Jiali Xie

IPC: C12N15/82 ,  C12N15/113

CPC classification number: C12N15/8234 ,  C12N15/113 ,  C12N15/8216 ,  C12N15/8222 ,  C12N15/8237

Abstract: The present invention provides DNA molecules and constructs, and their nucleotide sequences, useful for modulating gene expression in plants. Transgenic plants, plant cells, plant parts, and seeds comprising the DNA molecules operably linked to heterologous transcribable polynucleotides are also provided, as are methods of their use.

公开(公告)号：US20150307890A1

公开(公告)日：2015-10-29

申请号：US14685063

申请日：2015-04-13

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Wei Wu ,  Jack Tabaska ,  David Kovalic ,  Bo-Xing Qiu ,  Liang Guo

IPC: C12N15/82

CPC classification number: C12N15/8218 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8278

Abstract: This disclosure provides purified nucleic acids and polypeptides. Also provided are transgenic plants, seeds, and plant cells containing DNA for expression of the proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants, methods of making such plants and methods of making agricultural commodity including seeds and hybrid seeds from such plants.

Abstract translation: 本公开提供了纯化的核酸和多肽。 还提供了转基因植物，种子和含有用于表达可用于赋予转基因作物植物增强的农学性状的蛋白质的DNA的植物细胞，以及制造这种植物的方法以及制造包括种子和杂交种子在内的农产品的方法 从这样的植物。

公开(公告)号：US20150096083A1

公开(公告)日：2015-04-02

申请号：US14444966

申请日：2014-07-28

Applicant: Monsanto Technology LLC

Inventor： Wei Wu ,  Qi Wang ,  James Morrell ,  Linda Lutfiyya ,  Maolong Lu ,  Zhaolong Li ,  Ping Li ,  Michelle Lacy ,  David Kovalic ,  Yongwei Cao ,  Andrey Boukharov ,  Stanislaw Flasinski ,  Peter T. Hajdukiewicz

IPC: C12N15/82 ,  A01N57/20

CPC classification number: C12N15/8225 ,  A01N57/20 ,  C12N15/8222 ,  C12N15/8227 ,  C12N15/8229 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8286

Abstract: The present invention provides non-coding regulatory element polynucleotide molecules isolated from the lipid transfer protein (LTP) gene of Oryza sativa and useful for expressing transgenes in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the Oryza sativa regulatory polynucleotide sequences, and methods for preparing and using the same.

Abstract translation: 本发明提供从Oryza sativa的脂质转移蛋白（LTP）基因分离的非编码调节元件多核苷酸分子，并且可用于在植物中表达转基因。 本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有水稻调节多核苷酸序列的种子，以及制备和使用该方法。

公开(公告)号：US20130232646A1

公开(公告)日：2013-09-05

申请号：US13783125

申请日：2013-03-01

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Larry A. Gilbertson ,  David K. Kovalic ,  Thomas J. LaRosa ,  Maolong Lu ,  Tichafa R.I. Munyikwa ,  James K. Roberts ,  Wei Wu ,  Bei Zhang

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

Abstract translation: 本发明涉及通过抑制无脊椎害虫中的一种或多种生物学功能来控制害虫侵袭。 本发明公开了通过将一种或多种不同的重组双链RNA分子加入害虫来控制害虫侵染的方法和组合物，以通过抑制基因表达来实现害虫侵染的减少。 本发明还涉及用于制备表达双链RNA分子的转基因植物以及用于保护植物免受虫害侵袭的转基因杀虫剂的特定组合的方法。