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51.发明授权公开(公告)号：US09109211B2
公开(公告)日：2015-08-18
申请号：US13946229
申请日：2013-07-19
申请人： Novozymes A/S
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译： 本发明涉及具有植酸酶活性的多肽。 这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。 这些植酸酶具有改善的比活性。 还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能 。 本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。
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公开(公告)号：US20150191755A1
公开(公告)日：2015-07-09
申请号：US14412803
申请日：2013-07-18
申请人： Novozymes A/S
发明人： Weijian Lai , Ting Sun , Leonardo De Maria
IPC分类号： C12P7/62
CPC分类号： C12P7/62 , C12N9/24 , C12Y301/01074 , D06M16/003 , D06M2101/32
摘要： The present invention relates to the use of glycosyl hydrolase family 61 polypeptides in the presence of cutinases for polyester textile manufacture as well as a textile composition comprising glycosyl hydrolase family 61 polypeptides and cutinases.
摘要翻译： 本发明涉及在用于聚酯纺织品制造的角质酶存在下糖基水解酶家族61多肽的使用以及包含糖基水解酶家族61多肽和角质酶的纺织组合物。
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公开(公告)号：US20150159149A1
公开(公告)日：2015-06-11
申请号：US14627584
申请日：2015-02-20
申请人： NOVOZYMES A/S
CPC分类号： C12N9/96 , A23K10/16 , A23L33/195 , A61K38/46 , C12N9/16 , C12N15/8243 , C12P7/00 , C12P7/14 , C12P21/00 , C12Y301/03008 , C12Y301/03026 , C12Y301/03072
摘要： The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.
摘要翻译： 本发明涉及一种生产植酸酶变体的方法，所述植酸酶变体与来自柠檬酸杆菌（Citrobacter braakii）的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少两个另外的二硫键。 这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及产生的变体，编码这些植酸酶的DNA，其生产方法，以及其用途，例如在动物饲料和动物饲料添加剂中。
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公开(公告)号：US20140255550A1
公开(公告)日：2014-09-11
申请号：US14286089
申请日：2014-05-23
申请人： Novozymes A/S
发明人： Leonardo De Maria , Carsten Andersen , Lars Lehmann Hyllling Christensen , Soren Flensted Lassen , Peter Rahbek Ostergaard
CPC分类号： C12N9/58 , A01K2217/05 , A23K20/189 , C12N9/48 , C12N9/52 , C12N2310/111
摘要： The invention relates to a novel 3D structure encoding a Nocardiopsis protease, as well as to variants of parent protease homologous to Nocardiopsis proteases, preferably of improved thermostability and/or with an amended temperature activity profile. The invention also relates to DNA sequences encoding such variants, their production in a recombinant host cell, as well as methods of using the variants, in particular within the field of animal feed and detergents. The invention furthermore relates to methods of generating and preparing protease variants of amended properties.
摘要翻译： 本发明涉及编码诺卡氏菌蛋白酶的新型3D结构，以及与诺卡氏菌蛋白酶同源的亲本蛋白酶的变体，优选具有改善的热稳定性和/或具有修改的温度活性分布。 本发明还涉及编码这些变体的DNA序列，其在重组宿主细胞中的产生，以及使用该变体，特别是在动物饲料和洗涤剂领域中的方法。 本发明还涉及生成和制备修饰性质的蛋白酶变体的方法。
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55.发明申请公开(公告)号：US20140047571A1
公开(公告)日：2014-02-13
申请号：US14051945
申请日：2013-10-11
申请人： Novozymes A/S
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译： 本发明涉及具有植酸酶活性的多肽。 这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。 这些植酸酶具有改善的比活性。 还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能 。 本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。
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56.发明申请公开(公告)号：US20130298267A1
公开(公告)日：2013-11-07
申请号：US13946229
申请日：2013-07-19
申请人： Novozymes A/S
CPC分类号： C12N9/16 , A23L33/18 , C12N15/8243 , C12P7/06 , Y02E50/17
摘要： The present invention relates to polypeptides having phytase activity. These polypeptides have an amino acid sequence which has at least 70% identity to either of three phytases derived from the bacterium Buttiauxella, and which comprises at least one of the following amino acids at the position indicated: 119N, 120L, and/or 121E. These phytases have an improved specific activity. Additional specific amino acid substitutions are also disclosed which characterize and distinguish additional phytases of the invention having improved properties such as temperature and/or pH stability, pH activity profile, temperature activity profile, substrate profile, improved performance in animal feed in vitro or in vivo. The invention also relates to isolated polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译： 本发明涉及具有植酸酶活性的多肽。 这些多肽具有与来自细菌Buttiauxella的三种植酸酶中的任一种具有至少70％同一性的氨基酸序列，并且其在所示位置包含至少一个以下氨基酸：119N，120L和/或121E。 这些植酸酶具有改善的比活性。 还公开了另外的特定氨基酸取代，其特征和区分本发明的另外的植酸酶具有改善的性质如温度和/或pH稳定性，pH活性曲线，温度活性分布，底物分布，动物饲料体外或体内改善的性能 。 本发明还涉及编码多肽的分离的多核苷酸，核酸构建体，载体和包含多核苷酸的宿主细胞以及产生和使用多肽的方法。
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