公开(公告)号：US5266179A

公开(公告)日：1993-11-30

申请号：US733130

申请日：1991-07-19

申请人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

发明人： Shiro Nankai ,  Mariko Kawaguri ,  Toshihiko Yoshioka ,  Haruhiro Tsutsumi ,  Kyozo Terao ,  Naoki Tanimoto ,  Masahiro Yoshioka ,  Hiroshi Hyodo ,  Takatoshi Uchigaki

IPC分类号： G01N27/26 ,  G01N27/28 ,  G01N27/416 ,  G01N27/49

CPC分类号： G01N33/48771 ,  G01N2035/009

摘要： A sensor 13 is inserted into a connector 14. A constant voltage required to obtain a response current is applied across the connector 14 by a voltage applying source 15 at timings required. A response current of the sensor 13 inserted into the connector 14 is converted into a voltage by a current-to-voltage converter 16, and the amount thereof is determined by a microcomputer, the analysis results being displayed onto a display unit.

公开(公告)号：US5229282A

公开(公告)日：1993-07-20

申请号：US617886

申请日：1990-11-26

申请人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai

IPC分类号： C12Q1/00

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： The present invention relates to a process of preparation of a biosensor comprising forming an electrode system mainly containing carbon on an insulating base plate, treating the surface of electrode system with an organic solvent, and then arranging a reaction layer on the electrode system to give a unified element. The reaction layer contains an enzyme, electron acceptor and a hydrophilic polymer. Treatment with the organic solvent improves adhesion of the reaction layer to the electrode system. The electrode system contains a working electrode and a counter electrode. The electrode system is formed from a carbon paste containing a resin binder. Treatment is preferably carried out by wiping the surface of the electrodes with a material impregnated by the organic solvent.

公开(公告)号：US06867002B2

公开(公告)日：2005-03-15

申请号：US09420719

申请日：1999-10-20

申请人： Mariko Miyashita ,  Toshihiko Yoshioka ,  Shiro Nankai

发明人： Mariko Miyashita ,  Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N33/53 ,  G01N33/543

CPC分类号： C12Q1/001 ,  Y10S436/825 ,  Y10T436/11 ,  Y10T436/115831 ,  Y10T436/12 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： A sample solution treating instrument is provided for facilitating rapid and simplified adjustment of the condition of a sample solution proper for analysis with a biosensor before supplying the solution to the biosensor. The sample solution treating instrument includes, for example, a catalyst or an adsorbent which can remove any interfering substance in order to adjust the sample solution for measurement with a biosensor.

摘要翻译： 提供了一种样品溶液处理仪器，用于在将溶液供给生物传感器之前，利用生物传感器快速和简化调整适于分析的样品溶液的状况。 样品溶液处理仪器包括例如可以除去任何干扰物质的催化剂或吸附剂，以便通过生物传感器调节用于测量的样品溶液。

公开(公告)号：US5658443A

公开(公告)日：1997-08-19

申请号：US277556

申请日：1994-07-19

申请人： Tomohiro Yamamoto ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Satoko Fujisawa ,  Shiro Nankai

发明人： Tomohiro Yamamoto ,  Mariko Miyahara ,  Toshihiko Yoshioka ,  Satoko Fujisawa ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/002 ,  C12Q1/001 ,  Y10S435/817

摘要： A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.

摘要翻译： 用于以高精度快速定量包含在各种生物样品中的特定成分的生物传感器具有电绝缘基底，包括形成在绝缘基底的一个面上的工作电极和对电极的电极系统，以及形成在绝缘基底上的反应层 绝缘基座与电极系统紧密接触。 反应层至少含有亲水性聚合物，缓冲液和与缓冲液分离的酶。

公开(公告)号：US5512159A

公开(公告)日：1996-04-30

申请号：US293131

申请日：1994-08-19

申请人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

发明人： Toshihiko Yoshioka ,  Mariko Kawaguri ,  Shiro Nankai ,  Haruhiro Tsutsumi ,  Hideyuki Baba ,  Yoshinobu Tokuno ,  Shoji Miyazaki

IPC分类号： G01N27/28 ,  C12M1/34 ,  C12M1/40 ,  C12Q1/00 ,  C12Q1/54 ,  G01N27/327 ,  G01N33/483

CPC分类号： C12Q1/001

摘要： A biosensor of the present invention is provided with an insulating base plate, an electrode system mainly consisting of a working electrode and a counter electrode formed on the insulating base plate, and a reaction layer on the electrode system. The counter electrode is partially a circular arc. A manufacturing method of the biosensor of the present invention is comprised of a step to form a base by arranging leads, an electrode system, and an insulating layer on an insulating base plate, and a step to form a reaction layer mainly composed of an enzyme on the electrode system. Since the counter electrode is partially in the shape of a circular arc, the reaction layer is easily formed and prevented from delaminating, whereby the measuring accuracy, preservative properties and reliability of the biosensor are improved. The present invention enables highly efficient biosensors to be mass produced at low cost.

摘要翻译： 本发明的生物传感器设置有绝缘基板，主要由在绝缘基板上形成的工作电极和对电极组成的电极系统和电极系统上的反应层。 对电极部分是圆弧。 本发明的生物传感器的制造方法包括通过在绝缘基板上排列引线，电极系统和绝缘层形成基底的步骤，以及形成主要由酶组成的反应层的步骤 在电极系统上。 由于对电极部分为圆弧形状，反应层易于形成并防止分层，从而提高了生物传感器的测量精度，防腐性能和可靠性。 本发明能够以低成本批量生产高效生物传感器。

公开(公告)号：US5192415A

公开(公告)日：1993-03-09

申请号：US845073

申请日：1992-03-02

申请人： Toshihiko Yoshioka ,  Shiro Nankai

发明人： Toshihiko Yoshioka ,  Shiro Nankai

IPC分类号： C12Q1/00

CPC分类号： C12Q1/004

摘要： The biosensor of the present invention quantifies a substrate contained in a sample liquid by reducing electron acceptors using electron generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode, a reaction layer including the enzyme provided on the electrode system and a hydrogen ion concentration control layer, and the reaction layer is in contact with the electrode system. According to the present invention, the hydrogen ion concentration in the sample liquid can be made most appropriate in accordance with the type of the enzyme contained in the reaction layer, without the adjustment of the hydrogen ion concentration in the sample liquid beforehand. Thus, the specific substrate contained in the sample liquid can be easily quantified with accuracy and speed.

摘要翻译： 本发明的生物传感器通过使用在基板与酶的反应中产生的电子，然后通过电化学测量电子受体的量减少电子受体来量化包含在样品液体中的基板。 生物传感器具有电绝缘基板，至少包括工作电极和对电极的电极系，包含设置在电极系上的酶的反应层和氢离子浓度控制层，反应层与 电极系统。 根据本发明，不需要事先调整样品液中的氢离子浓度，可以根据反应层中所含的酶的种类，使样品液中的氢离子浓度最适合。 因此，可以容易地以准确度和速度量化包含在样品液体中的特定基质。

公开(公告)号：US07060168B2

公开(公告)日：2006-06-13

申请号：US10420893

申请日：2003-04-23

申请人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

发明人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： G01N27/327

CPC分类号： C12Q1/004

摘要： The present invention provides a biosensor having a favorable response characteristic up to a high concentration range, a low blank response and a high storage stability. This sensor comprises an electrode system including a working electrode and a counter electrode, for forming an electrochemical measurement system by coming in contact with a supplied solution; an electrically insulating supporting member for supporting the electrode system; a first reagent layer formed on the working electrode; and a second reagent layer formed on the counter electrode, wherein the first reagent layer comprises an enzyme as the main component, and the second reagent layer comprises an electron mediator as the main component.

公开(公告)号：US06878262B2

公开(公告)日：2005-04-12

申请号：US10216716

申请日：2002-08-13

申请人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

发明人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： A61B5/00 ,  A61B5/05 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/26 ,  G01N27/327 ,  G01N27/416 ,  G01N33/487

CPC分类号： C12Q1/005 ,  A61B5/1486 ,  A61B5/150022 ,  A61B5/150213 ,  A61B5/150297 ,  A61B5/150358 ,  A61B5/150412 ,  A61B5/150503 ,  A61B5/15142 ,  A61B5/15186 ,  A61B5/157 ,  G01N27/3272

摘要： The present invention provides an analytical element which is free from evaporation of a sample during measurement and therefore capable of quantifying a substrate using a very small amount of sample with high accuracy and which is free from scattering of the sample during and after the measurement and therefore hygienically excellent; and a measuring device and a substrate quantification method using the same. The analytical element comprises a cavity for accommodating a sample, a working electrode and a counter electrode exposed to an inside of the cavity, a reagent layer which comprises at least an oxidoreductase and is formed inside or in the vicinity of the cavity, an opening communicating with the cavity and a member covering the opening.

摘要翻译： 本发明提供一种在测量期间不会使样品蒸发的分析元件，因此能够使用非常少量的样品以高精度定量基底，并且在测量期间和之后不会发生样品散射，因此 卫生卓越 以及使用其的测量装置和基板定量方法。 分析元件包括用于容纳样品的空腔，暴露于空腔内部的工作电极和对电极，至少包含氧化还原酶并形成在空腔内部或附近的试剂层，开口连通 空腔和覆盖开口的构件。

公开(公告)号：US06458258B2

公开(公告)日：2002-10-01

申请号：US09748229

申请日：2000-12-27

申请人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

发明人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： G01N2726

CPC分类号： C12Q1/004 ,  C12Q1/001

摘要： The present invention provides a biosensor having a high current response sensitivity, a low blank response and a high storage stability. This sensor comprises an electrode system including a working electrode and a counter electrode, for forming an electrochemical measurement system by coming in contact with a supplied solution; an insulating supporting member for supporting the electrode system; a first reagent layer formed on the working electrode; and a second reagent layer formed on the counter electrode, wherein the first reagent layer does not contain an enzyme, but it contains at least an electron mediator, and the second reagent layer does not contain an electron mediator, but it contains at least an enzyme.

摘要翻译： 本发明提供具有高电流响应灵敏度，低空白响应和高储存稳定性的生物传感器。 该传感器包括电极系统，其包括工作电极和对电极，用于通过与所提供的溶液接触形成电化学测量系统; 用于支撑电极系统的绝缘支撑构件; 形成在工作电极上的第一试剂层; 和形成在对电极上的第二试剂层，其中第一试剂层不含酶，但至少包含电子介体，第二试剂层不含电子介体，但至少含有酶 。

公开(公告)号：US06885196B2

公开(公告)日：2005-04-26

申请号：US10220153

申请日：2001-07-17

申请人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

发明人： Yuko Taniike ,  Shin Ikeda ,  Shiro Nankai

IPC分类号： C12Q1/00 ,  A61B5/05

CPC分类号： G01N27/3272 ,  C12Q1/005 ,  C12Q1/006

摘要： A highly sensitive biosensor that uses a smaller amount of sample for measurement is described. The biosensor includes a first insulating base plate having a working electrode, a second insulating base plate having a counter electrode opposed to the working electrode, and a reagent layer containing at least an oxidoreductase. The biosensor further includes a sample supply pathway formed between the first and second insulating base plates, where the working electrode, counter electrode and reagent layer are exposed to an inside of the sample supply pathway, and the distance between the working electrode and the counter electrode is 150 μm or less.

摘要翻译： 描述了使用较少量的样品进行测量的高灵敏度生物传感器。 生物传感器包括具有工作电极的第一绝缘基板，具有与工作电极相对的反电极的第二绝缘基板和至少含有氧化还原酶的试剂层。 生物传感器还包括形成在第一和第二绝缘基板之间的样品供给路径，其中工作电极，对电极和试剂层暴露于样品供应路径的内部，并且工作电极和对电极之间的距离 是150mam以下。