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公开(公告)号：US20060121582A1
公开(公告)日：2006-06-08
申请号：US10528885
申请日：2003-09-22
申请人： Tatsuo Hoshino , Taro Miyazaki , Teruhide Sugisawa
发明人： Tatsuo Hoshino , Taro Miyazaki , Teruhide Sugisawa
CPC分类号： C12P17/04
摘要： The present invention relates to a process for the production of vitamin C from L sorbosone using an aldehyde dehydrogenase which is isolated from Gluconobacter oxydans DSM 4025 (FERM BP-3812), said enzyme having the following physicochemical properties: (a) molecular weight of 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da); (b) substrate specificity as active on aldehyde compounds; (c) Cofactors are pyrroloquinoline quinone and heme c; (d) Optimum pH between 6.4 and 8.2 for vitamin C production from L-sorbosone; and (e) as inhibitors Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and ethylendiamine tetraacetic acid. The process is performed in the presence of a suitable electron acceptor and the vitamin C isolated from the reaction mixture.
摘要翻译： 本发明涉及使用从脱氧氧化葡糖杆菌DSM 4025（FERM BP-3812）中分离的醛脱氢酶从L山梨糖酮生产维生素C的方法，所述酶具有以下物理化学性质：（a）分子量为15万 ±6,000Da或230,000±9,000Da（由2或3个同源亚基组成，每个亚基具有75,000±3,000Da的分子量）; （b）对醛化合物具有活性的底物特异性; （c）辅因子是吡咯并喹啉醌和血红素c; （d）从L-山梨糖酮生产维生素C的最佳pH在6.4和8.2之间; 和（e）作为抑制剂Co 2+，Cu 2+，Fe 2+，Ni 2+， Zn2 +，一碘乙酸酯和乙二胺四乙酸。 该方法在合适的电子受体和从反应混合物中分离的维生素C的存在下进行。
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公开(公告)号：US20060121557A1
公开(公告)日：2006-06-08
申请号：US10528848
申请日：2003-09-23
申请人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
CPC分类号： C12P23/00
摘要： Disclosed is a process for producing zeaxanthin and β-cryptoxanthin which comprises cultivating a recombinant microorganism which is expressing a β-carotene hydroxylase gene and belonging to the genus Xanthophyllomyces (Phaffia) in an aqueous nutrient medium under aerobic conditions, and isolating the resulted carotenoids from the cells of said recombinant microorganism or from the cultured broth.
摘要翻译： 公开了一种生产玉米黄质和β-隐黄质的方法，包括在需氧条件下，在含水营养培养基中培养表达β-胡萝卜素羟化酶基因并属于黄单胞菌属（Phanthia）属的重组微生物，并将得到的类胡萝卜素从 所述重组微生物的细胞或来自培养液的细胞。
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公开(公告)号：US20050244940A1
公开(公告)日：2005-11-03
申请号：US10528673
申请日：2003-09-22
申请人： Tatsuo Hoshino , Masako Shinjoh
发明人： Tatsuo Hoshino , Masako Shinjoh
摘要： The present invention provides the use of Enzyme B of G. oxydans DSM 4025, as disclosed in EP 0 832 974 A2, in a process for producing L-ascorbic acid from L-gulose, L-galactose, L-idose or L-talose, or from L-gulono-1,4-lactone (and its acid form, L-gulonic acid) and from L-galactono-1,4-lactone (and its acid form, L-galactonic acid).
摘要翻译： 本发明提供如EP 0 832 974 A2中公开的G.oxydans DSM 4025的酶B在L-古洛糖，L-半乳糖，L-阿糖或L-赖氨酸生产L-抗坏血酸的方法中的用途 ，或L-gulono-1,4-内酯（及其酸形式，L-古洛糖酸）和L-半乳糖-1,4-内酯（及其酸形式，L-半乳糖酸）。
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公开(公告)号：US20050090645A1
公开(公告)日：2005-04-28
申请号：US10802682
申请日：2004-03-17
摘要： The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or more recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore, the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).
摘要翻译： 本发明涉及具有醇和醛脱氢酶活性的重组酶，其包含一种或多种选自SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO： NO 8和作为SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO 8鉴定的至少两个以下氨基酸序列和上述多肽的功能衍生物的嵌合组合的嵌合重组多肽 其含有一个或多个氨基酸残基的添加，插入，缺失和/或取代，其中所述酶多肽具有所述醇和醛脱氢酶活性。 提供了编码重组多肽的DNA分子，包含这种DNA分子的载体，由这些载体转化的宿主细胞和用于产生这种重组酶的方法。 此外，具有醇和醛脱氢酶活性的重组酶用于获得醛，酮或羧酸，特别是2-酮-L-古洛糖酸是用于生产L-抗坏血酸（维生素C）的中间体。
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公开(公告)号：US06846660B2
公开(公告)日：2005-01-25
申请号：US09815695
申请日：2001-03-23
申请人： Tatsuo Hoshino , Taro Miyazaki , Teruhide Sugisawa
发明人： Tatsuo Hoshino , Taro Miyazaki , Teruhide Sugisawa
CPC分类号： C12N9/0008 , C12P7/60
摘要： A new aldehyde dehydrogenase having the physico-chemical properties: molecular weight:150,000±6,000 or 230,000±9,000; substrate specificity active on aldehyde compounds; cofactors:pyrroloquinoline quinone and heme c; optimum pH: 7.0-8.5; and inhibitors: Co2+, Cu2+, Fe2+, Ni2+, Zn2+, monoiodoacetate and EDTA, is derived from a microorganism belonging to the genus Gluconobacter. Said aldehyde dehydrogenase can be produced by cultivating a microorganism of the genus Gluconobacter which is capable of producing an aldehyde dehydrogenase having the above properties, in an aqueous nutrient medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifying the aldehyde dehydrogenase from the cell-free extract of the disrupted cells of the microorganism. 2-Keto-L-gulonic acid (2-KGA) can be produced from L-sorbosone by contacting L-sorbosone with (i) the aldehyde dehydrogenase in the presence of an electron acceptor, (ii) a Gluconobacter microorganism capable of producing the aldehyde dehydrogenase in an aqueous medium under aerobic conditions or (iii) a cell-free extract of said microorganism, and in each case isolating the resulting 2-KGA from the reaction mixture.
摘要翻译： 具有物理化学性质的新型醛脱氢酶：分子量：150,000±6,000或230,000±9,000; 对醛化合物具有活性的底物特异性; 辅因子：吡咯并喹啉醌和血红素c; 最适pH：7.0-8.5; 和抑制剂：Co 2+，Cu 2+，Fe 2+，Ni 2+，Zn 2+，一碘乙酸酯和EDTA，衍生自属于葡糖杆菌属的微生物。 所述醛脱氢酶可以通过在需氧条件下在水性营养培养基中培养能够产生具有上述性质的醛脱氢酶的葡糖杆菌属的微生物，破坏微生物的细胞并从其中分离和纯化醛脱氢酶 微生物破碎的细胞的无细胞提取物。 2-酮-L-古洛糖酸（2-KGA）可以由L-山梨糖酮通过在电子受体存在下将L-山梨醇与（i）醛脱氢酶接触，（ii）能产生 醛脱氢酶在有氧条件下在水性介质中或（iii）所述微生物的无细胞提取物，并且在每种情况下从反应混合物中分离得到的2-KGA。
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公开(公告)号：US06653115B1
公开(公告)日：2003-11-25
申请号：US09635145
申请日：2000-08-08
IPC分类号： C12N902
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
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公开(公告)号：US06586202B2
公开(公告)日：2003-07-01
申请号：US09925388
申请日：2001-08-09
申请人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12N988
CPC分类号： C12N9/0004 , C12N9/1085 , C12N9/1205 , C12N9/88 , C12P23/00
摘要： The present invention relates to an isolated DNA sequence encoding an enzyme in the mevalonate pathway or the pathway from isopentenyl pyrophosphate to farnesyl pyrophosphate. Vectors and plasmids including such DNA are also set forth. The invention also includes host cells transformed by such DNAs, or vectors or plasmids containing such DNAs. A process for the production of isoprenoids and carotenoids using such transformed host cells is also provided.
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公开(公告)号：US06365386B1
公开(公告)日：2002-04-02
申请号：US09518386
申请日：2000-03-03
申请人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
发明人： Tatsuo Hoshino , Kazuyuki Ojima , Yutaka Setoguchi
IPC分类号： C12N900
摘要： The present invention is directed to genetic materials useful for the preparation of astaxanthin from beta-carotene, such as polypeptides having astaxanthin synthase activity, DNA fragments coding for astaxanthin synthase, recombinant organisms and the like. Those novel genetic materials may be derived from Phaffia rhodozyma. The present invention also provides a process for the production of astaxanthin.
摘要翻译： 本发明涉及可用于从β-胡萝卜素制备虾青素的遗传物质，例如具有虾青素合酶活性的多肽，编码虾青素合酶的DNA片段，重组生物体等。 这些新型遗传物质可以衍生自红发夫酵母（Phaffia rhodozyma）。 本发明还提供了生产虾青素的方法。
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公开(公告)号：US06127156A
公开(公告)日：2000-10-03
申请号：US136251
申请日：1998-08-19
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12P19/02 , C12R1/01 , C12R1/02 , C12R1/19 , C07H21/04 , C12N1/20 , C12P21/06
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
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公开(公告)号：US5541108A
公开(公告)日：1996-07-30
申请号：US266998
申请日：1994-06-28
CPC分类号： C12N9/0006 , C12N15/03 , C12P7/60 , Y10S435/822
摘要： A process for producing 2-keto-L-gulonic acid which comprises converting L-sorbose and/or D-sorbitol into 2-keto-L-gulonic acid with the aid of a microorganism or its cell free extract, said microorganism belonging to the species Gluconobacter oxydans capable of producing 2-keto-L-gulonic acid and having L-sorbose dehydrogenase activity. Also disclosed are specific microorganisms useful in such process.
摘要翻译： 一种生产2-酮-L-古洛糖酸的方法，其包括借助于微生物或其无细胞提取物将L-山梨糖和/或D-山梨醇转化为2-酮-L-古洛糖酸，所述微生物属于 能产生2-酮-L-古洛糖酸并具有L-山梨糖脱氢酶活性的氧化葡糖杆菌属物种。 还公开了可用于该方法的特定微生物。
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