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公开(公告)号：US06444449B1
公开(公告)日：2002-09-03
申请号：US09634496
申请日：2000-08-08
IPC分类号： C12P1902
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
摘要翻译： 编码具有D-山梨糖醇脱氢酶活性的蛋白质或具有活化D-山梨糖醇脱氢酶蛋白质的体内，载体和含有此类多核苷酸的重组生物的多核苷酸，以及制备D-山梨醇脱氢酶和L-山梨糖的方法。
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公开(公告)号：US06653115B1
公开(公告)日：2003-11-25
申请号：US09635145
申请日：2000-08-08
IPC分类号： C12N902
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
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公开(公告)号：US06127156A
公开(公告)日：2000-10-03
申请号：US136251
申请日：1998-08-19
IPC分类号： C12N15/09 , C12N1/21 , C12N9/04 , C12P19/02 , C12R1/01 , C12R1/02 , C12R1/19 , C07H21/04 , C12N1/20 , C12P21/06
CPC分类号： C12N9/0006 , C12P19/02
摘要： Polynucleotides encoding proteins with D-sorbitol dehydrogenase activity or which function to activate D-sorbitol dehydrogenase proteins, in vivo, vectors and recombinant organisms which contain such polynucleotides, and methods of making D-sorbitol dehydrogenase and L-sorbose.
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公开(公告)号：US06730503B1
公开(公告)日：2004-05-04
申请号：US09470667
申请日：1999-12-22
IPC分类号： C12P740
摘要： The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or mom recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).
摘要翻译： 本发明涉及具有醇和醛脱氢酶活性的重组酶，其包含一种或多种选自SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO： NO 8和作为SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO 8鉴定的至少两个以下氨基酸序列和上述多肽的功能衍生物的嵌合组合的嵌合重组多肽 其含有一个或多个氨基酸残基的添加，插入，缺失和/或取代，其中所述酶多肽具有所述醇和醛脱氢酶活性。 提供了编码重组多肽的DNA分子，包含这种DNA分子的载体，由这些载体转化的宿主细胞和用于产生这种重组酶的方法。 此外，具有醇和醛脱氢酶活性的重组酶用于获得醛，酮或羧酸，特别是2-酮-L-古洛糖酸，用于生产L-抗坏血酸（维生素C）的中间体。
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公开(公告)号：US20050090645A1
公开(公告)日：2005-04-28
申请号：US10802682
申请日：2004-03-17
摘要： The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or more recombinant polypeptides selected from the group consisting of polypeptides which are identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and chimeric recombinant polypeptides that are a chimeric combination of at least two of the following amino acid sequences identified by SEQ ID NO 5, SEQ ID NO 6, SEQ ID NO 7, SEQ ID NO 8 and functional derivatives of the polypeptides identified above which contain addition, insertion, deletion and/or substitution of one or more amino acid residues, wherein said enzymatic polypeptides have said alcohol and aldehyde dehydrogenase activity. DNA molecules encoding the recombinant polypeptides, vectors comprising such DNA molecules, host cells transformed by such vectors and processes for the production of such recombinant enzymes are provided. Furthermore, the recombinant enzymes having alcohol and aldehyde dehydrogenase activity are used for obtaining aldehydes, ketones or carboxylic acids, and specifically, 2-keto-L-gulonic acid an intermediate for the production of L-ascorbic acid (vitamin C).
摘要翻译： 本发明涉及具有醇和醛脱氢酶活性的重组酶，其包含一种或多种选自SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO： NO 8和作为SEQ ID NO 5，SEQ ID NO 6，SEQ ID NO 7，SEQ ID NO 8鉴定的至少两个以下氨基酸序列和上述多肽的功能衍生物的嵌合组合的嵌合重组多肽 其含有一个或多个氨基酸残基的添加，插入，缺失和/或取代，其中所述酶多肽具有所述醇和醛脱氢酶活性。 提供了编码重组多肽的DNA分子，包含这种DNA分子的载体，由这些载体转化的宿主细胞和用于产生这种重组酶的方法。 此外，具有醇和醛脱氢酶活性的重组酶用于获得醛，酮或羧酸，特别是2-酮-L-古洛糖酸是用于生产L-抗坏血酸（维生素C）的中间体。
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公开(公告)号：US06407203B1
公开(公告)日：2002-06-18
申请号：US09474507
申请日：1999-12-29
IPC分类号： A61K3800
CPC分类号： C12N9/0006 , C07K14/80 , C12P17/04
摘要： This invention is directed to cytochrome c551 polypeptides and polynucleotides encoding the polypeptides.
摘要翻译： 本发明涉及细胞色素c551多肽和编码多肽的多核苷酸。
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公开(公告)号：US09279138B2
公开(公告)日：2016-03-08
申请号：US11883830
申请日：2006-02-10
CPC分类号： C12P17/04 , C12N9/0008 , C12P1/04
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of the polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of the polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in the microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中多核苷酸和/或编码多肽的修饰对产量，产量和/或 微生物发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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公开(公告)号：US20090142815A1
公开(公告)日：2009-06-04
申请号：US11883830
申请日：2006-02-10
CPC分类号： C12P17/04 , C12N9/0008 , C12P1/04
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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公开(公告)号：US20080113417A1
公开(公告)日：2008-05-15
申请号：US11883549
申请日：2006-02-10
CPC分类号： C12P17/04 , C12N9/0006
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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公开(公告)号：US6037147A
公开(公告)日：2000-03-14
申请号：US52830
申请日：1998-03-31
IPC分类号： C12N15/09 , C07D307/62 , C07K14/80 , C12N1/21 , C12N9/04 , C12N15/00 , C12P17/04 , C12P21/02 , C12R1/01 , C12R1/19 , C07H21/04 , C12N15/63 , C12N15/85 , C12P21/00
CPC分类号： C12N9/0006 , C07K14/80 , C12P17/04
摘要： This invention is directed to cytochrome c.sub.551 polypeptides and polynucleotides encoding the polypeptides.
摘要翻译： 本发明涉及细胞色素c551多肽和编码多肽的多核苷酸。
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