公开(公告)号：US20210265150A1

公开(公告)日：2021-08-26

申请号：US17255861

申请日：2019-06-28

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Don W Arnold ,  Thomas R Covey ,  Chang Liu

IPC: H01J49/04

Abstract: Methods and systems for delivering liquid sample to an ion source and subsequent analysis by mass spectrometry. In accordance with various aspects of the present teachings, MS-based systems and methods are provided in which desorption solvent is used in sampling interface to desorb analyte species from an SPME device that is coupled to an ion source to ionize analyte species desorbed into the desorption solvent for MS analysis (e.g., without a liquid chromatography (LC) column between the sampling interface and the ion source). In various aspects of the methods and systems described herein, configuring the sampling interface can be optimized so as to reduce the fluid volume dead space about the fluid inlet so as to concentrate the one or more analyte species desorbed at optimized conditions from the SPME substrate in a decreased volume of the desorption solvent when the SPME device is inserted into sampling interface.

公开(公告)号：US11067538B2

公开(公告)日：2021-07-20

申请号：US16088103

申请日：2017-03-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Bradley B. Schneider ,  Erkinjon Nazarov ,  Thomas R. Covey

IPC: G01N27/624 ,  H01J49/00 ,  H01J49/16

Abstract: An apparatus includes a first electrode and a second electrode. The second electrode is placed in parallel with the first electrode to provide constant gap distance. The gap between the first electrode and the second electrode is at atmospheric pressure. Ions are introduced into the center of the gap and travel through the apparatus in a direction parallel to the first electrode and the second electrode. The apparatus is configured as a high-field symmetric-waveform apparatus for filtering high mobility ions or for fragmenting ions. The apparatus is also configured for three modes of operation: as a conventional DMS; as a filter high mobility ions; and as fragmentation device. A symmetric electric field is produced in the gap with a maximum density normalized field strength greater than 10 Td to filter high mobility ions and with a maximum density normalized field strength greater than 100 Td to fragment ions.

公开(公告)号：US11024494B2

公开(公告)日：2021-06-01

申请号：US16646674

申请日：2018-09-25

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00 ,  H01J49/42

Abstract: An interference in a first MRM transition measurement for a compound of interest is determined by using a second MRM transition that includes an isotope of the precursor ion in the first MRM transition. Both transitions include the same product ion. A first intensity is measured for the first MRM transition and a second intensity is measured for the second MRM transition. A ratio of the first intensity to the second intensity is calculated. A theoretical ratio of the quantity of first precursor ion to the second precursor ion is calculated according to their isotopic relationship. A difference between the ratio and the theoretical ratio is calculated and compared to a threshold value. If the difference is less than the threshold value, the first intensity of the first MRM transition is identified as including an interference for the compound of interest.

公开(公告)号：US20210008575A1

公开(公告)日：2021-01-14

申请号：US16977210

申请日：2019-03-01

Applicant: DH Technologies Development Pte. Ltd. ,  Trajan Scientific Australia Pty Ltd

Inventor： John J. Corr ,  Thomas Covey ,  Peter Kovarik ,  Michael Bailey ,  Brett Barnett

IPC: B05B5/03 ,  H01J49/16 ,  G01N30/72

Abstract: An electrospray ionization emitter according to various aspects described herein can include an emitter body formed using fused silica. The emitter body can comprise a fluid conduit segment that includes a liquid connection end that has been coated with polyetheretherketone (PEEK) on at least one portion thereof. The liquid connection end can have a first outer diameter that is configured to be connected to a sample source to receive a sample liquid for ionization therefrom. The emitter body can further comprise an ionization discharge segment that is fluidly connected to the fluid conduit segment. The ionization discharge segment can have an ionization discharge end that is coated with a conductive material on at least one portion thereof and configured to have a second outer diameter that allows ionization of the liquid sample.

公开(公告)号：US20200279727A1

公开(公告)日：2020-09-03

申请号：US16063104

申请日：2016-12-15

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Takashi BABA ,  Paul BAKER ,  John Lawrence CAMPBELL ,  Yves LeBLANC

IPC: H01J49/00 ,  G01N33/92

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds and/or the orientation of those double bonds within the sample molecules. The fragments may include radical fragments and non-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules as confirmation of the presence of those radical fragments.

公开(公告)号：US10734212B2

公开(公告)日：2020-08-04

申请号：US15108203

申请日：2014-12-20

Applicant: DH Technologies Development PTE Ltd.

Inventor： Robert Haufler ,  William M. Loyd ,  Alexander Tsipirovich

IPC: H01J49/00 ,  H01J49/06 ,  H05H5/06 ,  H01J49/40 ,  H01J49/02 ,  H01J49/26

Abstract: A ring stacked accelerator for use in a mass spectrometer includes a plurality of ring shaped plates arranged in a stack and is electrically coupled to a voltage divider that allows a substantially homogeneous electric field to be produced when the stack is energized. The voltage divider can include resistors and capacitors, where the capacitors are chosen to compensate for parasitic capacitances experienced by the plates. The ring stacked accelerator can be energized using an RF pulse. The ring stack accelerator can include one or more balancing capacitors for correcting effects that cause nonlinearity.

公开(公告)号：US20200234936A1

公开(公告)日：2020-07-23

申请号：US16639161

申请日：2018-08-31

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Douglas Arnold Simmons ,  Nic G. Bloomfield

IPC: H01J49/00 ,  H01J49/06 ,  H01J49/42

Abstract: Dynamic skimmer pulsing and dynamic equilibration times are used for MS and MS/MS scans. A target percentage transmission of the ion beam is calculated based on a previous percentage transmission and a previous TIC or a previous highest intensity of a previous cycle time. An equilibration time is calculated based on the current percentage transmission and the target percentage transmission. A skimmer of a tandem mass spectrometer is controlled to attenuate the ion beam to the target percentage transmission to prevent saturation of a detector of the tandem mass spectrometer and to increase the dynamic range of the tandem mass spectrometer. The tandem mass spectrometer is controlled to perform an MS scan or an MS/MS scan after the calculated equilibration time to reduce the cycle time.

公开(公告)号：US20200185207A1

公开(公告)日：2020-06-11

申请号：US16790803

申请日：2020-02-14

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD. ,  Université Claude Bernard Lyon 1 ,  Centre National de la Recherche Scientifique ,  École Normale Supérieure de Lyon

Inventor： Jerome Lemoine

IPC: H01J49/00

Abstract: A plurality of MRM transitions to be used to monitor a sample are received and divided into two or more contiguous groups. At least one sentinel transition is selected in each group that identifies a next group of the two or more contiguous groups that is to be monitored. A first group of the two or more contiguous groups is placed on a duty cycle list of the tandem mass spectrometer. One or more compounds are separated from the sample and ionized, producing an ion beam. A series of MRM transitions read from the duty cycle list are executed on the ion beam by the tandem mass spectrometer. When at least one sentinel transition of the first group is detected, a next group identified by the sentinel transition is placed on the list.

公开(公告)号：US20190311892A1

公开(公告)日：2019-10-10

申请号：US16450594

申请日：2019-06-24

Applicant: DH Technologies Development Pte. Ltd. ,  ETH Zürich

Inventor： Ronald F. Bonner ,  Stephen A. Tate ,  Rudolf Aebersold ,  Pedro Jose Navarro Alvarez ,  Oliver Rinner ,  Lukas Reiter ,  Ludovic Gillet

IPC: H01J49/00 ,  G16C20/90 ,  G01N30/72 ,  H01J49/42

Abstract: Systems and methods are disclosed for analyzing a sample using overlapping precursor isolation windows. A mass analyzer of a tandem mass spectrometer is instructed to select and fragment at least two overlapping precursor isolation windows across a precursor ion mass range of a sample using a processor. The tandem mass spectrometer includes a mass analyzer that allows overlapping precursor isolation windows across the mass range of the sample.

公开(公告)号：US20190212337A1

公开(公告)日：2019-07-11

申请号：US16358533

申请日：2019-03-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Subhasish Purkayastha ,  Subhakar Dey

IPC: G01N33/569 ,  G01N33/68 ,  G01N30/88 ,  G01N30/72 ,  C12Q1/686 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/68

CPC classification number: G01N33/569 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/68 ,  C12Q1/686 ,  G01N30/72 ,  G01N30/7233 ,  G01N30/88 ,  G01N33/6848 ,  G01N2030/8831

Abstract: Systems and methods are provided for microbial identification using cleavable tags. Control information is sent to a mass spectrometer to fragment one or more nucleic acid primers labeled with a first tag and monitor for an intensity of the first tag in a mass spectrometry (MS) method. An ion source provides a beam of ions from a polymerase chain reaction amplified sample that includes one or more nucleic acid primers labeled with the first tag. The first tag binds to one or more nucleic acid primers of a known microbe and is cleaved from the nucleic acid primers during the MS method. The mass spectrometer receives the beam of ions and is adapted to perform the MS method on the beam of ions. If the intensity of the first tag received from the mass spectrometer exceeds a threshold value, the known microbe is identified in the sample.